In vivo tomographic imaging with fluorescence and MRI using tumor-targeted dual-labeled nanoparticles

Yue Zhang,1 Bin Zhang,1 Fei Liu,1,2 Jianwen Luo,1,3 Jing Bai1 1Department of Biomedical Engineering, School of Medicine, 2Tsinghua-Peking Center for Life Sciences, 3Center for Biomedical Imaging Research, Tsinghua University, Beijing, People's Republic of China Abstract: Dual-modality imag...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Zhang Y, Zhang B, Liu F, Luo J, Bai J
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2013
Materias:	Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/0543b38cb79248ddabb3b58acce51824
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:0543b38cb79248ddabb3b58acce51824
record_format	dspace
spelling	oai:doaj.org-article:0543b38cb79248ddabb3b58acce518242021-12-02T00:43:23ZIn vivo tomographic imaging with fluorescence and MRI using tumor-targeted dual-labeled nanoparticles1178-2013https://doaj.org/article/0543b38cb79248ddabb3b58acce518242013-12-01T00:00:00Zhttp://www.dovepress.com/in-vivo-tomographic-imaging-with-fluorescence-and-mri-using-tumor-targ-a15291https://doaj.org/toc/1178-2013 Yue Zhang,1 Bin Zhang,1 Fei Liu,1,2 Jianwen Luo,1,3 Jing Bai1 1Department of Biomedical Engineering, School of Medicine, 2Tsinghua-Peking Center for Life Sciences, 3Center for Biomedical Imaging Research, Tsinghua University, Beijing, People's Republic of China Abstract: Dual-modality imaging combines the complementary advantages of different modalities, and offers the prospect of improved preclinical research. The combination of fluorescence imaging and magnetic resonance imaging (MRI) provides cross-validated information and direct comparison between these modalities. Here, we report on the application of a novel tumor-targeted, dual-labeled nanoparticle (NP), utilizing iron oxide as the MRI contrast agent and near infrared (NIR) dye Cy5.5 as the fluorescent agent. Results of in vitro experiments verified the specificity of the NP to tumor cells. In vivo tumor targeting and uptake of the NPs in a mouse model were visualized by fluorescence and MR imaging collected at different time points. Quantitative analysis was carried out to evaluate the efficacy of MRI contrast enhancement. Furthermore, tomographic images were also acquired using both imaging modalities and cross-validated information of tumor location and size between these two modalities was revealed. The results demonstrate that the use of dual-labeled NPs can facilitate the dual-modal detection of tumors, information cross-validation, and direct comparison by combing fluorescence molecular tomography (FMT) and MRI. Keywords: dual-modality, fluorescence molecular tomography (FMT), magnetic resonance imaging (MRI), nanoparticleZhang YZhang BLiu FLuo JBai JDove Medical PressarticleMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol 2014, Iss Issue 1, Pp 33-41 (2013)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine (General) R5-920
spellingShingle	Medicine (General) R5-920 Zhang Y Zhang B Liu F Luo J Bai J In vivo tomographic imaging with fluorescence and MRI using tumor-targeted dual-labeled nanoparticles
description	Yue Zhang,1 Bin Zhang,1 Fei Liu,1,2 Jianwen Luo,1,3 Jing Bai1 1Department of Biomedical Engineering, School of Medicine, 2Tsinghua-Peking Center for Life Sciences, 3Center for Biomedical Imaging Research, Tsinghua University, Beijing, People's Republic of China Abstract: Dual-modality imaging combines the complementary advantages of different modalities, and offers the prospect of improved preclinical research. The combination of fluorescence imaging and magnetic resonance imaging (MRI) provides cross-validated information and direct comparison between these modalities. Here, we report on the application of a novel tumor-targeted, dual-labeled nanoparticle (NP), utilizing iron oxide as the MRI contrast agent and near infrared (NIR) dye Cy5.5 as the fluorescent agent. Results of in vitro experiments verified the specificity of the NP to tumor cells. In vivo tumor targeting and uptake of the NPs in a mouse model were visualized by fluorescence and MR imaging collected at different time points. Quantitative analysis was carried out to evaluate the efficacy of MRI contrast enhancement. Furthermore, tomographic images were also acquired using both imaging modalities and cross-validated information of tumor location and size between these two modalities was revealed. The results demonstrate that the use of dual-labeled NPs can facilitate the dual-modal detection of tumors, information cross-validation, and direct comparison by combing fluorescence molecular tomography (FMT) and MRI. Keywords: dual-modality, fluorescence molecular tomography (FMT), magnetic resonance imaging (MRI), nanoparticle
format	article
author	Zhang Y Zhang B Liu F Luo J Bai J
author_facet	Zhang Y Zhang B Liu F Luo J Bai J
author_sort	Zhang Y
title	In vivo tomographic imaging with fluorescence and MRI using tumor-targeted dual-labeled nanoparticles
title_short	In vivo tomographic imaging with fluorescence and MRI using tumor-targeted dual-labeled nanoparticles
title_full	In vivo tomographic imaging with fluorescence and MRI using tumor-targeted dual-labeled nanoparticles
title_fullStr	In vivo tomographic imaging with fluorescence and MRI using tumor-targeted dual-labeled nanoparticles
title_full_unstemmed	In vivo tomographic imaging with fluorescence and MRI using tumor-targeted dual-labeled nanoparticles
title_sort	in vivo tomographic imaging with fluorescence and mri using tumor-targeted dual-labeled nanoparticles
publisher	Dove Medical Press
publishDate	2013
url	https://doaj.org/article/0543b38cb79248ddabb3b58acce51824
work_keys_str_mv	AT zhangy invivotomographicimagingwithfluorescenceandmriusingtumortargetedduallabelednanoparticles AT zhangb invivotomographicimagingwithfluorescenceandmriusingtumortargetedduallabelednanoparticles AT liuf invivotomographicimagingwithfluorescenceandmriusingtumortargetedduallabelednanoparticles AT luoj invivotomographicimagingwithfluorescenceandmriusingtumortargetedduallabelednanoparticles AT baij invivotomographicimagingwithfluorescenceandmriusingtumortargetedduallabelednanoparticles
_version_	1718403475903086592

In vivo tomographic imaging with fluorescence and MRI using tumor-targeted dual-labeled nanoparticles

Ejemplares similares