Plasmodium falciparum Allelic Diversity: A Comparison of DNA Extraction from Isolates Collected on Rapid Diagnostic Tests (Rdts) and Filter Paper

Background: To perform molecular epidemiologic studies based on large cohorts, material such as RDTs or filter papers are useful for biological sample collection and extraction of RNA or DNA of good quality. Thus, we aimed to assess the quality of DNA extracted from malaria rapid diagnostic tests (R...

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Autores principales:	Denise Patricia Mawili-Mboumba, Marie Louise Tshibola Mbuyi, Noé Patrick M’Bondoukwe, Marielle Karine Bouyou Akotet
Formato:	article
Lenguaje:	EN
Publicado:	Tehran University of Medical Sciences 2021
Materias:	dna genetic diversity rapid diagnostic tests malaria Infectious and parasitic diseases RC109-216
Acceso en línea:	https://doaj.org/article/05452eb487e2434abe625c130d3dd673
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spelling	oai:doaj.org-article:05452eb487e2434abe625c130d3dd6732021-11-29T07:18:54ZPlasmodium falciparum Allelic Diversity: A Comparison of DNA Extraction from Isolates Collected on Rapid Diagnostic Tests (Rdts) and Filter Paper1735-70202008-238Xhttps://doaj.org/article/05452eb487e2434abe625c130d3dd6732021-11-01T00:00:00Zhttps://ijpa.tums.ac.ir/index.php/ijpa/article/view/3148https://doaj.org/toc/1735-7020https://doaj.org/toc/2008-238XBackground: To perform molecular epidemiologic studies based on large cohorts, material such as RDTs or filter papers are useful for biological sample collection and extraction of RNA or DNA of good quality. Thus, we aimed to assess the quality of DNA extracted from malaria rapid diagnostic tests (RDTs) stored at various temperatures for the analysis of Plasmodium falciparum genetic diversity. Methods: Febrile patients benefitted from free malaria diagnosis using microscopy in a malaria sentinel site, at the Regional Hospital Estuaire-Melen, in Gabon, in 2015. P. falciparum isolates were collected onto one filter paper and 2 similar RDTs devices (Acon®) per patient. Nucleic acids were extracted with QiAmp Qiagen kit from paper and RDTs and the quality of the DNA was analyzed by msp1 gene amplification. Results: Msp1gene amplification was achieved in nucleic acids extracted from all filter papers and RDTs devices (n = 45, 100%). K1 alleles were detected in 93.3% (n = 42/45) of the samples and Mad20 alleles in 73.3% (n = 33/45). The number and the intensity of K1 and/or Mad20 fragments were comparable according to the sample collection material and the storage conditions (room temperature vs -20°C) of the samples. The size of the fragments indicating allelic diversity was comparable in 80% (n=36) of the samples. Conclusion: These data show that RDTs are a valuable source of DNA for malaria parasite genetic polymorphism analysis. Storage conditions of the devices did not influence the quality of DNA extracted from RDTs device, although some alleles may be missed.Denise Patricia Mawili-MboumbaMarie Louise Tshibola MbuyiNoé Patrick M’BondoukweMarielle Karine Bouyou AkotetTehran University of Medical Sciencesarticlednagenetic diversityrapid diagnostic testsmalariaInfectious and parasitic diseasesRC109-216ENIranian Journal of Parasitology, Vol 16, Iss 4, Pp 555-559 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	dna genetic diversity rapid diagnostic tests malaria Infectious and parasitic diseases RC109-216
spellingShingle	dna genetic diversity rapid diagnostic tests malaria Infectious and parasitic diseases RC109-216 Denise Patricia Mawili-Mboumba Marie Louise Tshibola Mbuyi Noé Patrick M’Bondoukwe Marielle Karine Bouyou Akotet Plasmodium falciparum Allelic Diversity: A Comparison of DNA Extraction from Isolates Collected on Rapid Diagnostic Tests (Rdts) and Filter Paper
description	Background: To perform molecular epidemiologic studies based on large cohorts, material such as RDTs or filter papers are useful for biological sample collection and extraction of RNA or DNA of good quality. Thus, we aimed to assess the quality of DNA extracted from malaria rapid diagnostic tests (RDTs) stored at various temperatures for the analysis of Plasmodium falciparum genetic diversity. Methods: Febrile patients benefitted from free malaria diagnosis using microscopy in a malaria sentinel site, at the Regional Hospital Estuaire-Melen, in Gabon, in 2015. P. falciparum isolates were collected onto one filter paper and 2 similar RDTs devices (Acon®) per patient. Nucleic acids were extracted with QiAmp Qiagen kit from paper and RDTs and the quality of the DNA was analyzed by msp1 gene amplification. Results: Msp1gene amplification was achieved in nucleic acids extracted from all filter papers and RDTs devices (n = 45, 100%). K1 alleles were detected in 93.3% (n = 42/45) of the samples and Mad20 alleles in 73.3% (n = 33/45). The number and the intensity of K1 and/or Mad20 fragments were comparable according to the sample collection material and the storage conditions (room temperature vs -20°C) of the samples. The size of the fragments indicating allelic diversity was comparable in 80% (n=36) of the samples. Conclusion: These data show that RDTs are a valuable source of DNA for malaria parasite genetic polymorphism analysis. Storage conditions of the devices did not influence the quality of DNA extracted from RDTs device, although some alleles may be missed.
format	article
author	Denise Patricia Mawili-Mboumba Marie Louise Tshibola Mbuyi Noé Patrick M’Bondoukwe Marielle Karine Bouyou Akotet
author_facet	Denise Patricia Mawili-Mboumba Marie Louise Tshibola Mbuyi Noé Patrick M’Bondoukwe Marielle Karine Bouyou Akotet
author_sort	Denise Patricia Mawili-Mboumba
title	Plasmodium falciparum Allelic Diversity: A Comparison of DNA Extraction from Isolates Collected on Rapid Diagnostic Tests (Rdts) and Filter Paper
title_short	Plasmodium falciparum Allelic Diversity: A Comparison of DNA Extraction from Isolates Collected on Rapid Diagnostic Tests (Rdts) and Filter Paper
title_full	Plasmodium falciparum Allelic Diversity: A Comparison of DNA Extraction from Isolates Collected on Rapid Diagnostic Tests (Rdts) and Filter Paper
title_fullStr	Plasmodium falciparum Allelic Diversity: A Comparison of DNA Extraction from Isolates Collected on Rapid Diagnostic Tests (Rdts) and Filter Paper
title_full_unstemmed	Plasmodium falciparum Allelic Diversity: A Comparison of DNA Extraction from Isolates Collected on Rapid Diagnostic Tests (Rdts) and Filter Paper
title_sort	plasmodium falciparum allelic diversity: a comparison of dna extraction from isolates collected on rapid diagnostic tests (rdts) and filter paper
publisher	Tehran University of Medical Sciences
publishDate	2021
url	https://doaj.org/article/05452eb487e2434abe625c130d3dd673
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Plasmodium falciparum Allelic Diversity: A Comparison of DNA Extraction from Isolates Collected on Rapid Diagnostic Tests (Rdts) and Filter Paper

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