Characterization of the erythrocyte GTPase Rac1 in relation to Plasmodium falciparum invasion

Abstract Malaria is still a devastating disease with 228 million cases globally and 405,000 lethal outcomes in 2018, mainly in children under five years of age. The threat of emerging malaria strains resistant to currently available drugs has made the search for novel drug targets compelling. The pr...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Silvio Paone, Sarah D’Alessandro, Silvia Parapini, Francesco Celani, Valentina Tirelli, Manoochehr Pourshaban, Anna Olivieri
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2020
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/054f741b11594b4ba3f56720cd9c53cb
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:054f741b11594b4ba3f56720cd9c53cb
record_format	dspace
spelling	oai:doaj.org-article:054f741b11594b4ba3f56720cd9c53cb2021-12-02T13:34:01ZCharacterization of the erythrocyte GTPase Rac1 in relation to Plasmodium falciparum invasion10.1038/s41598-020-79052-02045-2322https://doaj.org/article/054f741b11594b4ba3f56720cd9c53cb2020-12-01T00:00:00Zhttps://doi.org/10.1038/s41598-020-79052-0https://doaj.org/toc/2045-2322Abstract Malaria is still a devastating disease with 228 million cases globally and 405,000 lethal outcomes in 2018, mainly in children under five years of age. The threat of emerging malaria strains resistant to currently available drugs has made the search for novel drug targets compelling. The process by which Plasmodium falciparum parasites invade the host cell has been widely studied, but only a few erythrocyte proteins involved in this process have been identified so far. The erythrocyte protein Rac1 is a GTPase that plays an important role in host cell invasion by many intracellular pathogens. Here we show that Rac1 is recruited in proximity to the site of parasite entry during P. falciparum invasion process and that subsequently localizes to the parasitophorous vacuole membrane. We also suggest that this GTPase may be involved in erythrocyte invasion by P. falciparum, by testing the effect of specific Rac1 inhibitory compounds. Finally, we suggest a secondary role of the erythrocyte GTPase also in parasite intracellular development. We here characterize a new erythrocyte protein potentially involved in P. falciparum invasion of the host cell and propose the human GTPase Rac1 as a novel and promising antimalarial drug target.Silvio PaoneSarah D’AlessandroSilvia ParapiniFrancesco CelaniValentina TirelliManoochehr PourshabanAnna OlivieriNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 10, Iss 1, Pp 1-12 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Silvio Paone Sarah D’Alessandro Silvia Parapini Francesco Celani Valentina Tirelli Manoochehr Pourshaban Anna Olivieri Characterization of the erythrocyte GTPase Rac1 in relation to Plasmodium falciparum invasion
description	Abstract Malaria is still a devastating disease with 228 million cases globally and 405,000 lethal outcomes in 2018, mainly in children under five years of age. The threat of emerging malaria strains resistant to currently available drugs has made the search for novel drug targets compelling. The process by which Plasmodium falciparum parasites invade the host cell has been widely studied, but only a few erythrocyte proteins involved in this process have been identified so far. The erythrocyte protein Rac1 is a GTPase that plays an important role in host cell invasion by many intracellular pathogens. Here we show that Rac1 is recruited in proximity to the site of parasite entry during P. falciparum invasion process and that subsequently localizes to the parasitophorous vacuole membrane. We also suggest that this GTPase may be involved in erythrocyte invasion by P. falciparum, by testing the effect of specific Rac1 inhibitory compounds. Finally, we suggest a secondary role of the erythrocyte GTPase also in parasite intracellular development. We here characterize a new erythrocyte protein potentially involved in P. falciparum invasion of the host cell and propose the human GTPase Rac1 as a novel and promising antimalarial drug target.
format	article
author	Silvio Paone Sarah D’Alessandro Silvia Parapini Francesco Celani Valentina Tirelli Manoochehr Pourshaban Anna Olivieri
author_facet	Silvio Paone Sarah D’Alessandro Silvia Parapini Francesco Celani Valentina Tirelli Manoochehr Pourshaban Anna Olivieri
author_sort	Silvio Paone
title	Characterization of the erythrocyte GTPase Rac1 in relation to Plasmodium falciparum invasion
title_short	Characterization of the erythrocyte GTPase Rac1 in relation to Plasmodium falciparum invasion
title_full	Characterization of the erythrocyte GTPase Rac1 in relation to Plasmodium falciparum invasion
title_fullStr	Characterization of the erythrocyte GTPase Rac1 in relation to Plasmodium falciparum invasion
title_full_unstemmed	Characterization of the erythrocyte GTPase Rac1 in relation to Plasmodium falciparum invasion
title_sort	characterization of the erythrocyte gtpase rac1 in relation to plasmodium falciparum invasion
publisher	Nature Portfolio
publishDate	2020
url	https://doaj.org/article/054f741b11594b4ba3f56720cd9c53cb
work_keys_str_mv	AT silviopaone characterizationoftheerythrocytegtpaserac1inrelationtoplasmodiumfalciparuminvasion AT sarahdalessandro characterizationoftheerythrocytegtpaserac1inrelationtoplasmodiumfalciparuminvasion AT silviaparapini characterizationoftheerythrocytegtpaserac1inrelationtoplasmodiumfalciparuminvasion AT francescocelani characterizationoftheerythrocytegtpaserac1inrelationtoplasmodiumfalciparuminvasion AT valentinatirelli characterizationoftheerythrocytegtpaserac1inrelationtoplasmodiumfalciparuminvasion AT manoochehrpourshaban characterizationoftheerythrocytegtpaserac1inrelationtoplasmodiumfalciparuminvasion AT annaolivieri characterizationoftheerythrocytegtpaserac1inrelationtoplasmodiumfalciparuminvasion
_version_	1718392775780597760

Characterization of the erythrocyte GTPase Rac1 in relation to Plasmodium falciparum invasion

Ejemplares similares