Microhomology-assisted scarless genome editing in human iPSCs

Positive selection for gene targeting is a common and reliable method to generate isogenic disease models in human pluripotent stem cells. Here, the authors present engineered selection markers which achieve scarless excision by CRISPR-Cas9 and microhomology mediated end-joining.

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Autores principales:	Shin-Il Kim, Tomoko Matsumoto, Harunobu Kagawa, Michiko Nakamura, Ryoko Hirohata, Ayano Ueno, Maki Ohishi, Tetsushi Sakuma, Tomoyoshi Soga, Takashi Yamamoto, Knut Woltjen
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2018
Materias:	Science Q
Acceso en línea:	https://doaj.org/article/0556a0b179e4474d9102ad13632d76a6
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spelling	oai:doaj.org-article:0556a0b179e4474d9102ad13632d76a62021-12-02T15:34:37ZMicrohomology-assisted scarless genome editing in human iPSCs10.1038/s41467-018-03044-y2041-1723https://doaj.org/article/0556a0b179e4474d9102ad13632d76a62018-03-01T00:00:00Zhttps://doi.org/10.1038/s41467-018-03044-yhttps://doaj.org/toc/2041-1723Positive selection for gene targeting is a common and reliable method to generate isogenic disease models in human pluripotent stem cells. Here, the authors present engineered selection markers which achieve scarless excision by CRISPR-Cas9 and microhomology mediated end-joining.Shin-Il KimTomoko MatsumotoHarunobu KagawaMichiko NakamuraRyoko HirohataAyano UenoMaki OhishiTetsushi SakumaTomoyoshi SogaTakashi YamamotoKnut WoltjenNature PortfolioarticleScienceQENNature Communications, Vol 9, Iss 1, Pp 1-14 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	Science Q
spellingShingle	Science Q Shin-Il Kim Tomoko Matsumoto Harunobu Kagawa Michiko Nakamura Ryoko Hirohata Ayano Ueno Maki Ohishi Tetsushi Sakuma Tomoyoshi Soga Takashi Yamamoto Knut Woltjen Microhomology-assisted scarless genome editing in human iPSCs
description	Positive selection for gene targeting is a common and reliable method to generate isogenic disease models in human pluripotent stem cells. Here, the authors present engineered selection markers which achieve scarless excision by CRISPR-Cas9 and microhomology mediated end-joining.
format	article
author	Shin-Il Kim Tomoko Matsumoto Harunobu Kagawa Michiko Nakamura Ryoko Hirohata Ayano Ueno Maki Ohishi Tetsushi Sakuma Tomoyoshi Soga Takashi Yamamoto Knut Woltjen
author_facet	Shin-Il Kim Tomoko Matsumoto Harunobu Kagawa Michiko Nakamura Ryoko Hirohata Ayano Ueno Maki Ohishi Tetsushi Sakuma Tomoyoshi Soga Takashi Yamamoto Knut Woltjen
author_sort	Shin-Il Kim
title	Microhomology-assisted scarless genome editing in human iPSCs
title_short	Microhomology-assisted scarless genome editing in human iPSCs
title_full	Microhomology-assisted scarless genome editing in human iPSCs
title_fullStr	Microhomology-assisted scarless genome editing in human iPSCs
title_full_unstemmed	Microhomology-assisted scarless genome editing in human iPSCs
title_sort	microhomology-assisted scarless genome editing in human ipscs
publisher	Nature Portfolio
publishDate	2018
url	https://doaj.org/article/0556a0b179e4474d9102ad13632d76a6
work_keys_str_mv	AT shinilkim microhomologyassistedscarlessgenomeeditinginhumanipscs AT tomokomatsumoto microhomologyassistedscarlessgenomeeditinginhumanipscs AT harunobukagawa microhomologyassistedscarlessgenomeeditinginhumanipscs AT michikonakamura microhomologyassistedscarlessgenomeeditinginhumanipscs AT ryokohirohata microhomologyassistedscarlessgenomeeditinginhumanipscs AT ayanoueno microhomologyassistedscarlessgenomeeditinginhumanipscs AT makiohishi microhomologyassistedscarlessgenomeeditinginhumanipscs AT tetsushisakuma microhomologyassistedscarlessgenomeeditinginhumanipscs AT tomoyoshisoga microhomologyassistedscarlessgenomeeditinginhumanipscs AT takashiyamamoto microhomologyassistedscarlessgenomeeditinginhumanipscs AT knutwoltjen microhomologyassistedscarlessgenomeeditinginhumanipscs
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Microhomology-assisted scarless genome editing in human iPSCs

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