Assessment of cyto-nephrotoxicity and growth performance in Labeo rohita induced by fluorescein dye Y and B

The cyto-nephrotoxicity in fishes induced by dyes is a global issue. Fluorescein dye has a wide range of applications in industry and biological laboratories. In vivo experiment was planned to assess the sublethal effects of Fluorescein dye, Eosin Y, and Eosin B in Labeo rohita. This freshwater fish...

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Autores principales:	Bilal Hussain, Hassan Sardar, Muhammad Sajad, Hira Yaseen, Sulman Shafeeq, K.A. Al-Ghanim, Ahmed Mustafa, Shahid Mahboob
Formato:	article
Lenguaje:	EN
Publicado:	Elsevier 2021
Materias:	Cytotoxicity Growth Labeo rohita Lethal Fluorescein Science (General) Q1-390
Acceso en línea:	https://doaj.org/article/055a73cc44fc4813a876c3251a63e4d6
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spelling	oai:doaj.org-article:055a73cc44fc4813a876c3251a63e4d62021-11-18T04:44:35ZAssessment of cyto-nephrotoxicity and growth performance in Labeo rohita induced by fluorescein dye Y and B1018-364710.1016/j.jksus.2021.101672https://doaj.org/article/055a73cc44fc4813a876c3251a63e4d62021-12-01T00:00:00Zhttp://www.sciencedirect.com/science/article/pii/S1018364721003347https://doaj.org/toc/1018-3647The cyto-nephrotoxicity in fishes induced by dyes is a global issue. Fluorescein dye has a wide range of applications in industry and biological laboratories. In vivo experiment was planned to assess the sublethal effects of Fluorescein dye, Eosin Y, and Eosin B in Labeo rohita. This freshwater fish was exposed to increasing concentrations (0.0011, 0.0023, and 0.0047 mg/L) of both dyes in the aquarium for definite time intervals. A dye-free trial was set as a control. Fish was anesthetized and dissected by cutting on the ventral side to remove a kidney. Cyto-nephrotoxicity was detected through histopathology. The fish's significant mortality was observed when exposed to 0.0023 mg/L (LC50) and 0.0047 mg/L concentration of Eosin Y dye. These trial groups showed many kidney tissues alterations such as glomerular, tubular epithelial cell's degeneration, a reduced lumen of tubules, vacuolation, absence of bowmen's space, necrosis, glomerulonephritis with increased per tubular space, shrinking of the glomerulus, congestion, clogging, and degeneration of tubules. Eosin B showed less mortality and tissue damage, including glomerular contraction and increased space between glomerulus and capsule. ANOVA showed a highly significant reduction in weight gain of fish at different treatments levels of Eosin Y, but less or none in the case of Eosin B. Increase in fish weight was found to be 66.3 ± 0.38 g when exposed to Eosin Y at 0.0047 mg/L, but it was 69.05 ± 0.63 g when exposed to Eosin B while it was almost identical 69.2 ± 0.23 g for both dye-free trial groups. The pair-wise comparison showed that exposure of the Eosin Y at treatment level 0.0047 mg/L was found to be highly toxic for the kidneys of Labeo rohita. Although both classes of dye are cytotoxic at small concentrations, Eosin Y is acute cyto-nephrotoxic and was found to be involved in growth retardation. To prevent society from fatal consequences, usage of dyes having acute toxicity must be banned or switched to alternates having no effects.Bilal HussainHassan SardarMuhammad SajadHira YaseenSulman ShafeeqK.A. Al-GhanimAhmed MustafaShahid MahboobElsevierarticleCytotoxicityGrowthLabeo rohitaLethalFluoresceinScience (General)Q1-390ENJournal of King Saud University: Science, Vol 33, Iss 8, Pp 101672- (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Cytotoxicity Growth Labeo rohita Lethal Fluorescein Science (General) Q1-390
spellingShingle	Cytotoxicity Growth Labeo rohita Lethal Fluorescein Science (General) Q1-390 Bilal Hussain Hassan Sardar Muhammad Sajad Hira Yaseen Sulman Shafeeq K.A. Al-Ghanim Ahmed Mustafa Shahid Mahboob Assessment of cyto-nephrotoxicity and growth performance in Labeo rohita induced by fluorescein dye Y and B
description	The cyto-nephrotoxicity in fishes induced by dyes is a global issue. Fluorescein dye has a wide range of applications in industry and biological laboratories. In vivo experiment was planned to assess the sublethal effects of Fluorescein dye, Eosin Y, and Eosin B in Labeo rohita. This freshwater fish was exposed to increasing concentrations (0.0011, 0.0023, and 0.0047 mg/L) of both dyes in the aquarium for definite time intervals. A dye-free trial was set as a control. Fish was anesthetized and dissected by cutting on the ventral side to remove a kidney. Cyto-nephrotoxicity was detected through histopathology. The fish's significant mortality was observed when exposed to 0.0023 mg/L (LC50) and 0.0047 mg/L concentration of Eosin Y dye. These trial groups showed many kidney tissues alterations such as glomerular, tubular epithelial cell's degeneration, a reduced lumen of tubules, vacuolation, absence of bowmen's space, necrosis, glomerulonephritis with increased per tubular space, shrinking of the glomerulus, congestion, clogging, and degeneration of tubules. Eosin B showed less mortality and tissue damage, including glomerular contraction and increased space between glomerulus and capsule. ANOVA showed a highly significant reduction in weight gain of fish at different treatments levels of Eosin Y, but less or none in the case of Eosin B. Increase in fish weight was found to be 66.3 ± 0.38 g when exposed to Eosin Y at 0.0047 mg/L, but it was 69.05 ± 0.63 g when exposed to Eosin B while it was almost identical 69.2 ± 0.23 g for both dye-free trial groups. The pair-wise comparison showed that exposure of the Eosin Y at treatment level 0.0047 mg/L was found to be highly toxic for the kidneys of Labeo rohita. Although both classes of dye are cytotoxic at small concentrations, Eosin Y is acute cyto-nephrotoxic and was found to be involved in growth retardation. To prevent society from fatal consequences, usage of dyes having acute toxicity must be banned or switched to alternates having no effects.
format	article
author	Bilal Hussain Hassan Sardar Muhammad Sajad Hira Yaseen Sulman Shafeeq K.A. Al-Ghanim Ahmed Mustafa Shahid Mahboob
author_facet	Bilal Hussain Hassan Sardar Muhammad Sajad Hira Yaseen Sulman Shafeeq K.A. Al-Ghanim Ahmed Mustafa Shahid Mahboob
author_sort	Bilal Hussain
title	Assessment of cyto-nephrotoxicity and growth performance in Labeo rohita induced by fluorescein dye Y and B
title_short	Assessment of cyto-nephrotoxicity and growth performance in Labeo rohita induced by fluorescein dye Y and B
title_full	Assessment of cyto-nephrotoxicity and growth performance in Labeo rohita induced by fluorescein dye Y and B
title_fullStr	Assessment of cyto-nephrotoxicity and growth performance in Labeo rohita induced by fluorescein dye Y and B
title_full_unstemmed	Assessment of cyto-nephrotoxicity and growth performance in Labeo rohita induced by fluorescein dye Y and B
title_sort	assessment of cyto-nephrotoxicity and growth performance in labeo rohita induced by fluorescein dye y and b
publisher	Elsevier
publishDate	2021
url	https://doaj.org/article/055a73cc44fc4813a876c3251a63e4d6
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Assessment of cyto-nephrotoxicity and growth performance in Labeo rohita induced by fluorescein dye Y and B

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