Cytokine biomarkers in tear film for primary open-angle glaucoma

Divakar Gupta,1,* Joanne C Wen,2,* Janet L Huebner,3 Sandra Stinnett,1 Virginia B Kraus,3,4 Henry C Tseng,1 Molly Walsh1 1Department of Ophthalmology, Duke University Medical Center, Durham, NC, 2Department of Ophthalmology, University of Washington, Seattle, WA, 3Duke Molecular Physiology Institut...

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Autores principales:	Gupta D, Wen JC, Huebner JL, Stinnett S, Kraus VB, Tseng HC, Walsh M
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2017
Materias:	cytokines tear film glaucoma diagnosis Ophthalmology RE1-994
Acceso en línea:	https://doaj.org/article/0564ff65c7654cc7a89e29fcf421ec9b
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spelling	oai:doaj.org-article:0564ff65c7654cc7a89e29fcf421ec9b2021-12-02T02:37:26ZCytokine biomarkers in tear film for primary open-angle glaucoma1177-5483https://doaj.org/article/0564ff65c7654cc7a89e29fcf421ec9b2017-02-01T00:00:00Zhttps://www.dovepress.com/cytokine-biomarkers-in-tear-film-for-primary-open-angle-glaucoma-peer-reviewed-article-OPTHhttps://doaj.org/toc/1177-5483Divakar Gupta,1,* Joanne C Wen,2,* Janet L Huebner,3 Sandra Stinnett,1 Virginia B Kraus,3,4 Henry C Tseng,1 Molly Walsh1 1Department of Ophthalmology, Duke University Medical Center, Durham, NC, 2Department of Ophthalmology, University of Washington, Seattle, WA, 3Duke Molecular Physiology Institute, 4Division of Rheumatology, Department of Medicine, Duke University School of Medicine, Durham, NC, USA *These authors contributed equally to this work Purpose: To determine the utility of tear film cytokines as biomarkers for early primary open-angle glaucoma (POAG). Methods: Patients without POAG and eye drop-naïve patients with newly diagnosed POAG were recruited from an academic hospital-based glaucoma practice. Tear films of recruited patients were obtained and analyzed using a multiplex, high-sensitivity electrochemiluminescent enzyme-linked immunosorbent assay for proinflammatory cytokines (IFNγ, IL-10, IL-12p70, IL-13, IL-1β, IL-2, IL-4, IL-6, IL-8, and TNFα). Results: Mean concentrations of tear film cytokines were lower in the glaucoma group for 8 of 10 cytokines tested. IL-12p70 (3.94±2.19 pg/mL in control vs 2.31±1.156 pg/mL in POAG; P=0.035) was significantly lower in the tear film of patients with newly diagnosed POAG. Conclusion: Proinflammatory cytokines were lower in eye drop-naïve newly diagnosed glaucoma patients. Tear film cytokine profiles may be used as biomarkers of early POAG. Keywords: glaucoma, biomarkers, tear film, cytokines, glaucoma diagnosis, lower limit of detectionGupta DWen JCHuebner JLStinnett SKraus VBTseng HCWalsh MDove Medical Pressarticlecytokinestear filmglaucoma diagnosisOphthalmologyRE1-994ENClinical Ophthalmology, Vol Volume 11, Pp 411-416 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	cytokines tear film glaucoma diagnosis Ophthalmology RE1-994
spellingShingle	cytokines tear film glaucoma diagnosis Ophthalmology RE1-994 Gupta D Wen JC Huebner JL Stinnett S Kraus VB Tseng HC Walsh M Cytokine biomarkers in tear film for primary open-angle glaucoma
description	Divakar Gupta,1,* Joanne C Wen,2,* Janet L Huebner,3 Sandra Stinnett,1 Virginia B Kraus,3,4 Henry C Tseng,1 Molly Walsh1 1Department of Ophthalmology, Duke University Medical Center, Durham, NC, 2Department of Ophthalmology, University of Washington, Seattle, WA, 3Duke Molecular Physiology Institute, 4Division of Rheumatology, Department of Medicine, Duke University School of Medicine, Durham, NC, USA *These authors contributed equally to this work Purpose: To determine the utility of tear film cytokines as biomarkers for early primary open-angle glaucoma (POAG). Methods: Patients without POAG and eye drop-naïve patients with newly diagnosed POAG were recruited from an academic hospital-based glaucoma practice. Tear films of recruited patients were obtained and analyzed using a multiplex, high-sensitivity electrochemiluminescent enzyme-linked immunosorbent assay for proinflammatory cytokines (IFNγ, IL-10, IL-12p70, IL-13, IL-1β, IL-2, IL-4, IL-6, IL-8, and TNFα). Results: Mean concentrations of tear film cytokines were lower in the glaucoma group for 8 of 10 cytokines tested. IL-12p70 (3.94±2.19 pg/mL in control vs 2.31±1.156 pg/mL in POAG; P=0.035) was significantly lower in the tear film of patients with newly diagnosed POAG. Conclusion: Proinflammatory cytokines were lower in eye drop-naïve newly diagnosed glaucoma patients. Tear film cytokine profiles may be used as biomarkers of early POAG. Keywords: glaucoma, biomarkers, tear film, cytokines, glaucoma diagnosis, lower limit of detection
format	article
author	Gupta D Wen JC Huebner JL Stinnett S Kraus VB Tseng HC Walsh M
author_facet	Gupta D Wen JC Huebner JL Stinnett S Kraus VB Tseng HC Walsh M
author_sort	Gupta D
title	Cytokine biomarkers in tear film for primary open-angle glaucoma
title_short	Cytokine biomarkers in tear film for primary open-angle glaucoma
title_full	Cytokine biomarkers in tear film for primary open-angle glaucoma
title_fullStr	Cytokine biomarkers in tear film for primary open-angle glaucoma
title_full_unstemmed	Cytokine biomarkers in tear film for primary open-angle glaucoma
title_sort	cytokine biomarkers in tear film for primary open-angle glaucoma
publisher	Dove Medical Press
publishDate	2017
url	https://doaj.org/article/0564ff65c7654cc7a89e29fcf421ec9b
work_keys_str_mv	AT guptad cytokinebiomarkersintearfilmforprimaryopenangleglaucoma AT wenjc cytokinebiomarkersintearfilmforprimaryopenangleglaucoma AT huebnerjl cytokinebiomarkersintearfilmforprimaryopenangleglaucoma AT stinnetts cytokinebiomarkersintearfilmforprimaryopenangleglaucoma AT krausvb cytokinebiomarkersintearfilmforprimaryopenangleglaucoma AT tsenghc cytokinebiomarkersintearfilmforprimaryopenangleglaucoma AT walshm cytokinebiomarkersintearfilmforprimaryopenangleglaucoma
_version_	1718402265734184960

Cytokine biomarkers in tear film for primary open-angle glaucoma

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