Multi-pass transmission electron microscopy

Feynman once asked physicists to build better electron microscopes to be able to watch biology at work. While electron microscopes can now provide atomic resolution, electron beam induced specimen damage precludes high resolution imaging of sensitive materials, such as single proteins or polymers. H...

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Autores principales:	Thomas Juffmann, Stewart A. Koppell, Brannon B. Klopfer, Colin Ophus, Robert M. Glaeser, Mark A. Kasevich
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/05664e9adc6440389dfa58b953d0c968
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spelling	oai:doaj.org-article:05664e9adc6440389dfa58b953d0c9682021-12-02T16:06:02ZMulti-pass transmission electron microscopy10.1038/s41598-017-01841-x2045-2322https://doaj.org/article/05664e9adc6440389dfa58b953d0c9682017-05-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-01841-xhttps://doaj.org/toc/2045-2322Feynman once asked physicists to build better electron microscopes to be able to watch biology at work. While electron microscopes can now provide atomic resolution, electron beam induced specimen damage precludes high resolution imaging of sensitive materials, such as single proteins or polymers. Here, we use simulations to show that an electron microscope based on a multi-pass measurement protocol enables imaging of single proteins, without averaging structures over multiple images. While we demonstrate the method for particular imaging targets, the approach is broadly applicable and is expected to improve resolution and sensitivity for a range of electron microscopy imaging modalities, including, for example, scanning and spectroscopic techniques. The approach implements a quantum mechanically optimal strategy which under idealized conditions can be considered interaction-free.Thomas JuffmannStewart A. KoppellBrannon B. KlopferColin OphusRobert M. GlaeserMark A. KasevichNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-7 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Thomas Juffmann Stewart A. Koppell Brannon B. Klopfer Colin Ophus Robert M. Glaeser Mark A. Kasevich Multi-pass transmission electron microscopy
description	Feynman once asked physicists to build better electron microscopes to be able to watch biology at work. While electron microscopes can now provide atomic resolution, electron beam induced specimen damage precludes high resolution imaging of sensitive materials, such as single proteins or polymers. Here, we use simulations to show that an electron microscope based on a multi-pass measurement protocol enables imaging of single proteins, without averaging structures over multiple images. While we demonstrate the method for particular imaging targets, the approach is broadly applicable and is expected to improve resolution and sensitivity for a range of electron microscopy imaging modalities, including, for example, scanning and spectroscopic techniques. The approach implements a quantum mechanically optimal strategy which under idealized conditions can be considered interaction-free.
format	article
author	Thomas Juffmann Stewart A. Koppell Brannon B. Klopfer Colin Ophus Robert M. Glaeser Mark A. Kasevich
author_facet	Thomas Juffmann Stewart A. Koppell Brannon B. Klopfer Colin Ophus Robert M. Glaeser Mark A. Kasevich
author_sort	Thomas Juffmann
title	Multi-pass transmission electron microscopy
title_short	Multi-pass transmission electron microscopy
title_full	Multi-pass transmission electron microscopy
title_fullStr	Multi-pass transmission electron microscopy
title_full_unstemmed	Multi-pass transmission electron microscopy
title_sort	multi-pass transmission electron microscopy
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/05664e9adc6440389dfa58b953d0c968
work_keys_str_mv	AT thomasjuffmann multipasstransmissionelectronmicroscopy AT stewartakoppell multipasstransmissionelectronmicroscopy AT brannonbklopfer multipasstransmissionelectronmicroscopy AT colinophus multipasstransmissionelectronmicroscopy AT robertmglaeser multipasstransmissionelectronmicroscopy AT markakasevich multipasstransmissionelectronmicroscopy
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Multi-pass transmission electron microscopy

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