Activity-Dependent Regulation of Alternative Cleavage and Polyadenylation During Hippocampal Long-Term Potentiation

Abstract Long-lasting forms of synaptic plasticity that underlie learning and memory require new transcription and translation for their persistence. The remarkable polarity and compartmentalization of neurons raises questions about the spatial and temporal regulation of gene expression within neuro...

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Autores principales: Mariana M. Fontes, Aysegul Guvenek, Riki Kawaguchi, Dinghai Zheng, Alden Huang, Victoria M. Ho, Patrick B. Chen, Xiaochuan Liu, Thomas J. O’Dell, Giovanni Coppola, Bin Tian, Kelsey C. Martin
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Publicado: Nature Portfolio 2017
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Acceso en línea:https://doaj.org/article/056a72711b3b4b43aa3da7c4e92037c8
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spelling oai:doaj.org-article:056a72711b3b4b43aa3da7c4e92037c82021-12-02T15:05:12ZActivity-Dependent Regulation of Alternative Cleavage and Polyadenylation During Hippocampal Long-Term Potentiation10.1038/s41598-017-17407-w2045-2322https://doaj.org/article/056a72711b3b4b43aa3da7c4e92037c82017-12-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-17407-whttps://doaj.org/toc/2045-2322Abstract Long-lasting forms of synaptic plasticity that underlie learning and memory require new transcription and translation for their persistence. The remarkable polarity and compartmentalization of neurons raises questions about the spatial and temporal regulation of gene expression within neurons. Alternative cleavage and polyadenylation (APA) generates mRNA isoforms with different 3′ untranslated regions (3′UTRs) and/or coding sequences. Changes in the 3′UTR composition of mRNAs can alter gene expression by regulating transcript localization, stability and/or translation, while changes in the coding sequences lead to mRNAs encoding distinct proteins. Using specialized 3′ end deep sequencing methods, we undertook a comprehensive analysis of APA following induction of long-term potentiation (LTP) of mouse hippocampal CA3-CA1 synapses. We identified extensive LTP-induced APA changes, including a general trend of 3′UTR shortening and activation of intronic APA isoforms. Comparison with transcriptome profiling indicated that most APA regulatory events were uncoupled from changes in transcript abundance. We further show that specific APA regulatory events can impact expression of two molecules with known functions during LTP, including 3′UTR APA of Notch1 and intronic APA of Creb1. Together, our results reveal that activity-dependent APA provides an important layer of gene regulation during learning and memory.Mariana M. FontesAysegul GuvenekRiki KawaguchiDinghai ZhengAlden HuangVictoria M. HoPatrick B. ChenXiaochuan LiuThomas J. O’DellGiovanni CoppolaBin TianKelsey C. MartinNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-13 (2017)
institution DOAJ
collection DOAJ
language EN
topic Medicine
R
Science
Q
spellingShingle Medicine
R
Science
Q
Mariana M. Fontes
Aysegul Guvenek
Riki Kawaguchi
Dinghai Zheng
Alden Huang
Victoria M. Ho
Patrick B. Chen
Xiaochuan Liu
Thomas J. O’Dell
Giovanni Coppola
Bin Tian
Kelsey C. Martin
Activity-Dependent Regulation of Alternative Cleavage and Polyadenylation During Hippocampal Long-Term Potentiation
description Abstract Long-lasting forms of synaptic plasticity that underlie learning and memory require new transcription and translation for their persistence. The remarkable polarity and compartmentalization of neurons raises questions about the spatial and temporal regulation of gene expression within neurons. Alternative cleavage and polyadenylation (APA) generates mRNA isoforms with different 3′ untranslated regions (3′UTRs) and/or coding sequences. Changes in the 3′UTR composition of mRNAs can alter gene expression by regulating transcript localization, stability and/or translation, while changes in the coding sequences lead to mRNAs encoding distinct proteins. Using specialized 3′ end deep sequencing methods, we undertook a comprehensive analysis of APA following induction of long-term potentiation (LTP) of mouse hippocampal CA3-CA1 synapses. We identified extensive LTP-induced APA changes, including a general trend of 3′UTR shortening and activation of intronic APA isoforms. Comparison with transcriptome profiling indicated that most APA regulatory events were uncoupled from changes in transcript abundance. We further show that specific APA regulatory events can impact expression of two molecules with known functions during LTP, including 3′UTR APA of Notch1 and intronic APA of Creb1. Together, our results reveal that activity-dependent APA provides an important layer of gene regulation during learning and memory.
format article
author Mariana M. Fontes
Aysegul Guvenek
Riki Kawaguchi
Dinghai Zheng
Alden Huang
Victoria M. Ho
Patrick B. Chen
Xiaochuan Liu
Thomas J. O’Dell
Giovanni Coppola
Bin Tian
Kelsey C. Martin
author_facet Mariana M. Fontes
Aysegul Guvenek
Riki Kawaguchi
Dinghai Zheng
Alden Huang
Victoria M. Ho
Patrick B. Chen
Xiaochuan Liu
Thomas J. O’Dell
Giovanni Coppola
Bin Tian
Kelsey C. Martin
author_sort Mariana M. Fontes
title Activity-Dependent Regulation of Alternative Cleavage and Polyadenylation During Hippocampal Long-Term Potentiation
title_short Activity-Dependent Regulation of Alternative Cleavage and Polyadenylation During Hippocampal Long-Term Potentiation
title_full Activity-Dependent Regulation of Alternative Cleavage and Polyadenylation During Hippocampal Long-Term Potentiation
title_fullStr Activity-Dependent Regulation of Alternative Cleavage and Polyadenylation During Hippocampal Long-Term Potentiation
title_full_unstemmed Activity-Dependent Regulation of Alternative Cleavage and Polyadenylation During Hippocampal Long-Term Potentiation
title_sort activity-dependent regulation of alternative cleavage and polyadenylation during hippocampal long-term potentiation
publisher Nature Portfolio
publishDate 2017
url https://doaj.org/article/056a72711b3b4b43aa3da7c4e92037c8
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