GM3 Ganglioside Linked to Neurofibrillary Pathology in a Transgenic Rat Model for Tauopathy

Glycosphingolipids (GSLs) are amphipathic lipids composed of a sphingoid base and a fatty acyl attached to a saccharide moiety. GSLs play an important role in signal transduction, directing proteins within the membrane, cell recognition, and modulation of cell adhesion. Gangliosides and sulfatides b...

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Autores principales:	Dominika Olešová, Petra Majerová, Roman Hájek, Juraj Piešťanský, Radana Brumarová, Alena Michalicová, Bernadeta Jurkanin, David Friedecký, Andrej Kováč
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	glycosphingolipids gangliosides sulfatides tauopathy neurodegeneration aging Biology (General) QH301-705.5 Chemistry QD1-999
Acceso en línea:	https://doaj.org/article/056d16036b764d01ae4fad39bd93653a
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spelling	oai:doaj.org-article:056d16036b764d01ae4fad39bd93653a2021-11-25T17:58:03ZGM3 Ganglioside Linked to Neurofibrillary Pathology in a Transgenic Rat Model for Tauopathy10.3390/ijms2222125811422-00671661-6596https://doaj.org/article/056d16036b764d01ae4fad39bd93653a2021-11-01T00:00:00Zhttps://www.mdpi.com/1422-0067/22/22/12581https://doaj.org/toc/1661-6596https://doaj.org/toc/1422-0067Glycosphingolipids (GSLs) are amphipathic lipids composed of a sphingoid base and a fatty acyl attached to a saccharide moiety. GSLs play an important role in signal transduction, directing proteins within the membrane, cell recognition, and modulation of cell adhesion. Gangliosides and sulfatides belong to a group of acidic GSLs, and numerous studies report their involvement in neurodevelopment, aging, and neurodegeneration. In this study, we used an approach based on hydrophilic interaction liquid chromatography (HILIC) coupled to high-resolution tandem mass spectrometry (HRMS/MS) to characterize the glycosphingolipid profile in rat brain tissue. Then, we screened characterized lipids aiming to identify changes in glycosphingolipid profiles in the normal aging process and tau pathology. Thorough screening of acidic glycosphingolipids in rat brain tissue revealed 117 ganglioside and 36 sulfatide species. Moreover, we found two ganglioside subclasses that were not previously characterized—GT1b-Ac2 and GQ1b-Ac2. The semi-targeted screening revealed significant changes in the levels of sulfatides and GM1a gangliosides during the aging process. In the transgenic SHR24 rat model for tauopathies, we found elevated levels of GM3 gangliosides which may indicate a higher rate of apoptotic processes.Dominika OlešováPetra MajerováRoman HájekJuraj PiešťanskýRadana BrumarováAlena MichalicováBernadeta JurkaninDavid FriedeckýAndrej KováčMDPI AGarticleglycosphingolipidsgangliosidessulfatidestauopathyneurodegenerationagingBiology (General)QH301-705.5ChemistryQD1-999ENInternational Journal of Molecular Sciences, Vol 22, Iss 12581, p 12581 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	glycosphingolipids gangliosides sulfatides tauopathy neurodegeneration aging Biology (General) QH301-705.5 Chemistry QD1-999
spellingShingle	glycosphingolipids gangliosides sulfatides tauopathy neurodegeneration aging Biology (General) QH301-705.5 Chemistry QD1-999 Dominika Olešová Petra Majerová Roman Hájek Juraj Piešťanský Radana Brumarová Alena Michalicová Bernadeta Jurkanin David Friedecký Andrej Kováč GM3 Ganglioside Linked to Neurofibrillary Pathology in a Transgenic Rat Model for Tauopathy
description	Glycosphingolipids (GSLs) are amphipathic lipids composed of a sphingoid base and a fatty acyl attached to a saccharide moiety. GSLs play an important role in signal transduction, directing proteins within the membrane, cell recognition, and modulation of cell adhesion. Gangliosides and sulfatides belong to a group of acidic GSLs, and numerous studies report their involvement in neurodevelopment, aging, and neurodegeneration. In this study, we used an approach based on hydrophilic interaction liquid chromatography (HILIC) coupled to high-resolution tandem mass spectrometry (HRMS/MS) to characterize the glycosphingolipid profile in rat brain tissue. Then, we screened characterized lipids aiming to identify changes in glycosphingolipid profiles in the normal aging process and tau pathology. Thorough screening of acidic glycosphingolipids in rat brain tissue revealed 117 ganglioside and 36 sulfatide species. Moreover, we found two ganglioside subclasses that were not previously characterized—GT1b-Ac2 and GQ1b-Ac2. The semi-targeted screening revealed significant changes in the levels of sulfatides and GM1a gangliosides during the aging process. In the transgenic SHR24 rat model for tauopathies, we found elevated levels of GM3 gangliosides which may indicate a higher rate of apoptotic processes.
format	article
author	Dominika Olešová Petra Majerová Roman Hájek Juraj Piešťanský Radana Brumarová Alena Michalicová Bernadeta Jurkanin David Friedecký Andrej Kováč
author_facet	Dominika Olešová Petra Majerová Roman Hájek Juraj Piešťanský Radana Brumarová Alena Michalicová Bernadeta Jurkanin David Friedecký Andrej Kováč
author_sort	Dominika Olešová
title	GM3 Ganglioside Linked to Neurofibrillary Pathology in a Transgenic Rat Model for Tauopathy
title_short	GM3 Ganglioside Linked to Neurofibrillary Pathology in a Transgenic Rat Model for Tauopathy
title_full	GM3 Ganglioside Linked to Neurofibrillary Pathology in a Transgenic Rat Model for Tauopathy
title_fullStr	GM3 Ganglioside Linked to Neurofibrillary Pathology in a Transgenic Rat Model for Tauopathy
title_full_unstemmed	GM3 Ganglioside Linked to Neurofibrillary Pathology in a Transgenic Rat Model for Tauopathy
title_sort	gm3 ganglioside linked to neurofibrillary pathology in a transgenic rat model for tauopathy
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/056d16036b764d01ae4fad39bd93653a
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GM3 Ganglioside Linked to Neurofibrillary Pathology in a Transgenic Rat Model for Tauopathy

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