Frequent detection of human adenovirus from the lower gastrointestinal tract in men who have sex with men.

<h4>Background</h4>The association between baseline seropositivity to human adenovirus (HAdV) type 5 and increased HIV acquisition in the Step HIV Vaccine Study has raised questions concerning frequency of acquired and/or persistent Adenovirus infections among adults at high risk of HIV-...

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Autores principales:	Marcel E Curlin, Meei-Li Huang, Xiaoyan Lu, Connie L Celum, Jorge Sanchez, Stacy Selke, Jared M Baeten, Richard A Zuckerman, Dean D Erdman, Lawrence Corey
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2010
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/056e5b960bd44d4288f6ffb5976d4f09
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spelling	oai:doaj.org-article:056e5b960bd44d4288f6ffb5976d4f092021-12-02T20:20:30ZFrequent detection of human adenovirus from the lower gastrointestinal tract in men who have sex with men.1932-620310.1371/journal.pone.0011321https://doaj.org/article/056e5b960bd44d4288f6ffb5976d4f092010-06-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/20593015/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203<h4>Background</h4>The association between baseline seropositivity to human adenovirus (HAdV) type 5 and increased HIV acquisition in the Step HIV Vaccine Study has raised questions concerning frequency of acquired and/or persistent Adenovirus infections among adults at high risk of HIV-1 infection.<h4>Methodology</h4>To evaluate the frequency and pattern of HAdV shedding from the lower GI tract, we retrospectively tested rectal swabs for HAdVs in a cohort of 20 HSV-2 positive HIV-positive Peruvian men who have sex with men (MSM) undergoing rectal swabbing three times/week for 18 consecutive weeks, in a prospective study of HSV-2 suppression in HIV infection. Viral DNA was extracted and amplified using a sensitive multiplex PCR assay that detects all currently recognized HAdV types. Molecular typing of viruses was performed on selected samples by hexon gene sequencing. Baseline neutralizing antibody titers to HAdVs -5, -26, -35 and -48 were also assessed.<h4>Principal findings</h4>15/20 individuals had HAdV detected during follow up. The median frequency of HAdV detection was 30% of samples (range 2.0% to 64.7%). HAdV shedding typically occurred on consecutive days in clustered episodes lasting a median of 4 days (range 1 to 9 days) separated by periods without shedding, suggesting frequent new infections or reactivation of latent infections over time. 8 of the 15 shedders had more than one type detected in follow-up. 20 HAdV types from species B, C, and D were identified, including HAdV-5, -26 and -48, HAdV types under development as potential vaccine candidates. 14/20 subjects were seropositive for HAdV-5; 15/20 for HAdV-26; 3/20 for HAdV-35; and 2/20 for HAdV-48. HAdV shedding did not correlate with CD4 count, plasma HIV-1 viral load, or titers to HAdV-5 or HAdV-35. The sole individual with HAdV-5 shedding was HAdV-5 seropositive.<h4>Conclusions</h4>HAdV shedding was highly prevalent and diverse, including types presently under consideration as HIV vaccine vectors. Subclinical HAdV infection of the GI tract is common among MSM in Peru; the prevalence of HAdV in the enteric tract should be evaluated in other populations. The association between ongoing recent enteric HAdV and the immune response to recombinant HAdV vaccines should be evaluated.Marcel E CurlinMeei-Li HuangXiaoyan LuConnie L CelumJorge SanchezStacy SelkeJared M BaetenRichard A ZuckermanDean D ErdmanLawrence CoreyPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 5, Iss 6, p e11321 (2010)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Marcel E Curlin Meei-Li Huang Xiaoyan Lu Connie L Celum Jorge Sanchez Stacy Selke Jared M Baeten Richard A Zuckerman Dean D Erdman Lawrence Corey Frequent detection of human adenovirus from the lower gastrointestinal tract in men who have sex with men.
description	<h4>Background</h4>The association between baseline seropositivity to human adenovirus (HAdV) type 5 and increased HIV acquisition in the Step HIV Vaccine Study has raised questions concerning frequency of acquired and/or persistent Adenovirus infections among adults at high risk of HIV-1 infection.<h4>Methodology</h4>To evaluate the frequency and pattern of HAdV shedding from the lower GI tract, we retrospectively tested rectal swabs for HAdVs in a cohort of 20 HSV-2 positive HIV-positive Peruvian men who have sex with men (MSM) undergoing rectal swabbing three times/week for 18 consecutive weeks, in a prospective study of HSV-2 suppression in HIV infection. Viral DNA was extracted and amplified using a sensitive multiplex PCR assay that detects all currently recognized HAdV types. Molecular typing of viruses was performed on selected samples by hexon gene sequencing. Baseline neutralizing antibody titers to HAdVs -5, -26, -35 and -48 were also assessed.<h4>Principal findings</h4>15/20 individuals had HAdV detected during follow up. The median frequency of HAdV detection was 30% of samples (range 2.0% to 64.7%). HAdV shedding typically occurred on consecutive days in clustered episodes lasting a median of 4 days (range 1 to 9 days) separated by periods without shedding, suggesting frequent new infections or reactivation of latent infections over time. 8 of the 15 shedders had more than one type detected in follow-up. 20 HAdV types from species B, C, and D were identified, including HAdV-5, -26 and -48, HAdV types under development as potential vaccine candidates. 14/20 subjects were seropositive for HAdV-5; 15/20 for HAdV-26; 3/20 for HAdV-35; and 2/20 for HAdV-48. HAdV shedding did not correlate with CD4 count, plasma HIV-1 viral load, or titers to HAdV-5 or HAdV-35. The sole individual with HAdV-5 shedding was HAdV-5 seropositive.<h4>Conclusions</h4>HAdV shedding was highly prevalent and diverse, including types presently under consideration as HIV vaccine vectors. Subclinical HAdV infection of the GI tract is common among MSM in Peru; the prevalence of HAdV in the enteric tract should be evaluated in other populations. The association between ongoing recent enteric HAdV and the immune response to recombinant HAdV vaccines should be evaluated.
format	article
author	Marcel E Curlin Meei-Li Huang Xiaoyan Lu Connie L Celum Jorge Sanchez Stacy Selke Jared M Baeten Richard A Zuckerman Dean D Erdman Lawrence Corey
author_facet	Marcel E Curlin Meei-Li Huang Xiaoyan Lu Connie L Celum Jorge Sanchez Stacy Selke Jared M Baeten Richard A Zuckerman Dean D Erdman Lawrence Corey
author_sort	Marcel E Curlin
title	Frequent detection of human adenovirus from the lower gastrointestinal tract in men who have sex with men.
title_short	Frequent detection of human adenovirus from the lower gastrointestinal tract in men who have sex with men.
title_full	Frequent detection of human adenovirus from the lower gastrointestinal tract in men who have sex with men.
title_fullStr	Frequent detection of human adenovirus from the lower gastrointestinal tract in men who have sex with men.
title_full_unstemmed	Frequent detection of human adenovirus from the lower gastrointestinal tract in men who have sex with men.
title_sort	frequent detection of human adenovirus from the lower gastrointestinal tract in men who have sex with men.
publisher	Public Library of Science (PLoS)
publishDate	2010
url	https://doaj.org/article/056e5b960bd44d4288f6ffb5976d4f09
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Frequent detection of human adenovirus from the lower gastrointestinal tract in men who have sex with men.

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