Precision modification of the human gut microbiota targeting surface-associated proteins

Abstract This work describes a new procedure that allows the targeted modification of the human gut microbiota by using antibodies raised against bacterial surface-associated proteins specific to the microorganism of interest. To this end, a polyclonal antibody recognising the surface-associated pro...

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Autores principales:	Raquel Marcos-Fernández, Lorena Ruiz, Aitor Blanco-Míguez, Abelardo Margolles, Borja Sánchez
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/056fe660968848479ec3b4ad956f7310
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spelling	oai:doaj.org-article:056fe660968848479ec3b4ad956f73102021-12-02T14:12:47ZPrecision modification of the human gut microbiota targeting surface-associated proteins10.1038/s41598-020-80187-32045-2322https://doaj.org/article/056fe660968848479ec3b4ad956f73102021-01-01T00:00:00Zhttps://doi.org/10.1038/s41598-020-80187-3https://doaj.org/toc/2045-2322Abstract This work describes a new procedure that allows the targeted modification of the human gut microbiota by using antibodies raised against bacterial surface-associated proteins specific to the microorganism of interest. To this end, a polyclonal antibody recognising the surface-associated protein Surface Layer Protein A of Lactobacillus acidophilus DSM20079T was developed. By conjugating this antibody with fluorescent probes and magnetic particles, we were able to specifically identify this bacterium both in a synthetic, and in real gut microbiotas by means of a flow cytometry approach. Further, we demonstrated the applicability of this antibody to deplete complex human gut microbiotas from L. acidophilus in a single step. L. acidophilus was found to interact with other bacteria both in synthetic and in real microbiotas, as reflected by its concomitant depletion together with other species. Further optimization of the procedure including a trypsin step enabled to achieve the selective and complete isolation of this species. Depleting a single species from a gut microbiota, using antibodies recognizing specific cell surface elements of the target organism, will open up novel ways to tackle research on the specific immunomodulatory and metabolic contributions of a bacterium of interest in the context of a complex human gut microbiota, including the investigation into therapeutic applications by adding/depleting a key bacterium. This represents the first work in which an antibody/flow-cytometry based application enabled the targeted edition of human gut microbiotas, and represents the basis for the design of precision microbiome-based therapies.Raquel Marcos-FernándezLorena RuizAitor Blanco-MíguezAbelardo MargollesBorja SánchezNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-13 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Raquel Marcos-Fernández Lorena Ruiz Aitor Blanco-Míguez Abelardo Margolles Borja Sánchez Precision modification of the human gut microbiota targeting surface-associated proteins
description	Abstract This work describes a new procedure that allows the targeted modification of the human gut microbiota by using antibodies raised against bacterial surface-associated proteins specific to the microorganism of interest. To this end, a polyclonal antibody recognising the surface-associated protein Surface Layer Protein A of Lactobacillus acidophilus DSM20079T was developed. By conjugating this antibody with fluorescent probes and magnetic particles, we were able to specifically identify this bacterium both in a synthetic, and in real gut microbiotas by means of a flow cytometry approach. Further, we demonstrated the applicability of this antibody to deplete complex human gut microbiotas from L. acidophilus in a single step. L. acidophilus was found to interact with other bacteria both in synthetic and in real microbiotas, as reflected by its concomitant depletion together with other species. Further optimization of the procedure including a trypsin step enabled to achieve the selective and complete isolation of this species. Depleting a single species from a gut microbiota, using antibodies recognizing specific cell surface elements of the target organism, will open up novel ways to tackle research on the specific immunomodulatory and metabolic contributions of a bacterium of interest in the context of a complex human gut microbiota, including the investigation into therapeutic applications by adding/depleting a key bacterium. This represents the first work in which an antibody/flow-cytometry based application enabled the targeted edition of human gut microbiotas, and represents the basis for the design of precision microbiome-based therapies.
format	article
author	Raquel Marcos-Fernández Lorena Ruiz Aitor Blanco-Míguez Abelardo Margolles Borja Sánchez
author_facet	Raquel Marcos-Fernández Lorena Ruiz Aitor Blanco-Míguez Abelardo Margolles Borja Sánchez
author_sort	Raquel Marcos-Fernández
title	Precision modification of the human gut microbiota targeting surface-associated proteins
title_short	Precision modification of the human gut microbiota targeting surface-associated proteins
title_full	Precision modification of the human gut microbiota targeting surface-associated proteins
title_fullStr	Precision modification of the human gut microbiota targeting surface-associated proteins
title_full_unstemmed	Precision modification of the human gut microbiota targeting surface-associated proteins
title_sort	precision modification of the human gut microbiota targeting surface-associated proteins
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/056fe660968848479ec3b4ad956f7310
work_keys_str_mv	AT raquelmarcosfernandez precisionmodificationofthehumangutmicrobiotatargetingsurfaceassociatedproteins AT lorenaruiz precisionmodificationofthehumangutmicrobiotatargetingsurfaceassociatedproteins AT aitorblancomiguez precisionmodificationofthehumangutmicrobiotatargetingsurfaceassociatedproteins AT abelardomargolles precisionmodificationofthehumangutmicrobiotatargetingsurfaceassociatedproteins AT borjasanchez precisionmodificationofthehumangutmicrobiotatargetingsurfaceassociatedproteins
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Precision modification of the human gut microbiota targeting surface-associated proteins

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