Gene expression changes in the injured spinal cord following transplantation of mesenchymal stem cells or olfactory ensheathing cells.

Transplantation of bone marrow derived mesenchymal stromal cells (MSC) or olfactory ensheathing cells (OEC) have demonstrated beneficial effects after spinal cord injury (SCI), providing tissue protection and improving the functional recovery. However, the changes induced by these cells after their...

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Autores principales: Abel Torres-Espín, Joaquim Hernández, Xavier Navarro
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Publicado: Public Library of Science (PLoS) 2013
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Acceso en línea:https://doaj.org/article/05708a9e0a8a4aac842d4073923c0575
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spelling oai:doaj.org-article:05708a9e0a8a4aac842d4073923c05752021-11-18T08:51:29ZGene expression changes in the injured spinal cord following transplantation of mesenchymal stem cells or olfactory ensheathing cells.1932-620310.1371/journal.pone.0076141https://doaj.org/article/05708a9e0a8a4aac842d4073923c05752013-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/24146830/?tool=EBIhttps://doaj.org/toc/1932-6203Transplantation of bone marrow derived mesenchymal stromal cells (MSC) or olfactory ensheathing cells (OEC) have demonstrated beneficial effects after spinal cord injury (SCI), providing tissue protection and improving the functional recovery. However, the changes induced by these cells after their transplantation into the injured spinal cord remain largely unknown. We analyzed the changes in the spinal cord transcriptome after a contusion injury and MSC or OEC transplantation. The cells were injected immediately or 7 days after the injury. The mRNA of the spinal cord injured segment was extracted and analyzed by microarray at 2 and 7 days after cell grafting. The gene profiles were analyzed by clustering and functional enrichment analysis based on the Gene Ontology database. We found that both MSC and OEC transplanted acutely after injury induce an early up-regulation of genes related to tissue protection and regeneration. In contrast, cells transplanted at 7 days after injury down-regulate genes related to tissue regeneration. The most important change after MSC or OEC transplant was a marked increase in expression of genes associated with foreign body response and adaptive immune response. These data suggest a regulatory effect of MSC and OEC transplantation after SCI regarding tissue repair processes, but a fast rejection response to the grafted cells. Our results provide an initial step to determine the mechanisms of action and to optimize cell therapy for SCI.Abel Torres-EspínJoaquim HernándezXavier NavarroPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 8, Iss 10, p e76141 (2013)
institution DOAJ
collection DOAJ
language EN
topic Medicine
R
Science
Q
spellingShingle Medicine
R
Science
Q
Abel Torres-Espín
Joaquim Hernández
Xavier Navarro
Gene expression changes in the injured spinal cord following transplantation of mesenchymal stem cells or olfactory ensheathing cells.
description Transplantation of bone marrow derived mesenchymal stromal cells (MSC) or olfactory ensheathing cells (OEC) have demonstrated beneficial effects after spinal cord injury (SCI), providing tissue protection and improving the functional recovery. However, the changes induced by these cells after their transplantation into the injured spinal cord remain largely unknown. We analyzed the changes in the spinal cord transcriptome after a contusion injury and MSC or OEC transplantation. The cells were injected immediately or 7 days after the injury. The mRNA of the spinal cord injured segment was extracted and analyzed by microarray at 2 and 7 days after cell grafting. The gene profiles were analyzed by clustering and functional enrichment analysis based on the Gene Ontology database. We found that both MSC and OEC transplanted acutely after injury induce an early up-regulation of genes related to tissue protection and regeneration. In contrast, cells transplanted at 7 days after injury down-regulate genes related to tissue regeneration. The most important change after MSC or OEC transplant was a marked increase in expression of genes associated with foreign body response and adaptive immune response. These data suggest a regulatory effect of MSC and OEC transplantation after SCI regarding tissue repair processes, but a fast rejection response to the grafted cells. Our results provide an initial step to determine the mechanisms of action and to optimize cell therapy for SCI.
format article
author Abel Torres-Espín
Joaquim Hernández
Xavier Navarro
author_facet Abel Torres-Espín
Joaquim Hernández
Xavier Navarro
author_sort Abel Torres-Espín
title Gene expression changes in the injured spinal cord following transplantation of mesenchymal stem cells or olfactory ensheathing cells.
title_short Gene expression changes in the injured spinal cord following transplantation of mesenchymal stem cells or olfactory ensheathing cells.
title_full Gene expression changes in the injured spinal cord following transplantation of mesenchymal stem cells or olfactory ensheathing cells.
title_fullStr Gene expression changes in the injured spinal cord following transplantation of mesenchymal stem cells or olfactory ensheathing cells.
title_full_unstemmed Gene expression changes in the injured spinal cord following transplantation of mesenchymal stem cells or olfactory ensheathing cells.
title_sort gene expression changes in the injured spinal cord following transplantation of mesenchymal stem cells or olfactory ensheathing cells.
publisher Public Library of Science (PLoS)
publishDate 2013
url https://doaj.org/article/05708a9e0a8a4aac842d4073923c0575
work_keys_str_mv AT abeltorresespin geneexpressionchangesintheinjuredspinalcordfollowingtransplantationofmesenchymalstemcellsorolfactoryensheathingcells
AT joaquimhernandez geneexpressionchangesintheinjuredspinalcordfollowingtransplantationofmesenchymalstemcellsorolfactoryensheathingcells
AT xaviernavarro geneexpressionchangesintheinjuredspinalcordfollowingtransplantationofmesenchymalstemcellsorolfactoryensheathingcells
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