Influence of luminescent graphene quantum dots on trypsin activity

Tanveer A Tabish,1,2 Md Zahidul I Pranjol,2 Ilayda Karadag,1 David W Horsell,1 Jacqueline L Whatmore,2 Shaowei Zhang1 1College of Engineering, Mathematics and Physical Sciences, University of Exeter, Exeter, UK; 2Institute of Biomedical and Clinical Science, University of Exeter Medical School, Exe...

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Autores principales:	Tabish TA, Pranjol MZI, Karadag I, Horsell DW, Whatmore JL, Zhang S
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2018
Materias:	graphene enzyme luminescence bioavailability surface energy Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/05791b8a1362406c9a7fd10dad983a88
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spelling	oai:doaj.org-article:05791b8a1362406c9a7fd10dad983a882021-12-02T02:20:03ZInfluence of luminescent graphene quantum dots on trypsin activity1178-2013https://doaj.org/article/05791b8a1362406c9a7fd10dad983a882018-03-01T00:00:00Zhttps://www.dovepress.com/influence-of-luminescent-graphene-quantum-dots-on-trypsin-activity-peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013Tanveer A Tabish,1,2 Md Zahidul I Pranjol,2 Ilayda Karadag,1 David W Horsell,1 Jacqueline L Whatmore,2 Shaowei Zhang1 1College of Engineering, Mathematics and Physical Sciences, University of Exeter, Exeter, UK; 2Institute of Biomedical and Clinical Science, University of Exeter Medical School, Exeter, UK Background: Protein–graphene interactions have the potential to play a pivotal role in the future directions of nanomedicine. These interactions lead to diverse processes such as generation of protein coronas, nano–bio interfaces, particle wrapping, and biocatalytic processes that could determine the ultimate fate of graphene nanocomposites in biologic systems. However, such interactions and their effects on the bioavailability of graphene have not yet been widely appreciated, despite the fact that this is the primary surface in contact with cells.Methods: This paper reports on the integrative physiochemical interaction between trypsin and graphene quantum dots (GQDs) to determine their potential biologic identity in enzyme engineering. This interaction was measured by a wide range of analytical methods.Results: Definitive binding and modulation of trypsin–GQDs was demonstrated for the first time by use of vibrational spectroscopy and wetting transparency, which revealed that trypsin was absorbed on GQDs’ surface through its cationic and hydrophilic residues. Our findings suggested that trypsin’s active sites were stabilized and protected by the GQDs, which were likely to be responsible for the high bioavailability of GQDs in enzymes.Conclusion: Our work demonstrates the efficacy of GQDs as an enzyme modulator with high specificity, and their great application potential in enzyme engineering as well as enzyme-based therapies. Keywords: graphene, enzyme, luminescence, bioavailability, surface energy Tabish TAPranjol MZIKaradag IHorsell DWWhatmore JLZhang SDove Medical Pressarticlegrapheneenzymeluminescencebioavailabilitysurface energyMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol Volume 13, Pp 1525-1538 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	graphene enzyme luminescence bioavailability surface energy Medicine (General) R5-920
spellingShingle	graphene enzyme luminescence bioavailability surface energy Medicine (General) R5-920 Tabish TA Pranjol MZI Karadag I Horsell DW Whatmore JL Zhang S Influence of luminescent graphene quantum dots on trypsin activity
description	Tanveer A Tabish,1,2 Md Zahidul I Pranjol,2 Ilayda Karadag,1 David W Horsell,1 Jacqueline L Whatmore,2 Shaowei Zhang1 1College of Engineering, Mathematics and Physical Sciences, University of Exeter, Exeter, UK; 2Institute of Biomedical and Clinical Science, University of Exeter Medical School, Exeter, UK Background: Protein–graphene interactions have the potential to play a pivotal role in the future directions of nanomedicine. These interactions lead to diverse processes such as generation of protein coronas, nano–bio interfaces, particle wrapping, and biocatalytic processes that could determine the ultimate fate of graphene nanocomposites in biologic systems. However, such interactions and their effects on the bioavailability of graphene have not yet been widely appreciated, despite the fact that this is the primary surface in contact with cells.Methods: This paper reports on the integrative physiochemical interaction between trypsin and graphene quantum dots (GQDs) to determine their potential biologic identity in enzyme engineering. This interaction was measured by a wide range of analytical methods.Results: Definitive binding and modulation of trypsin–GQDs was demonstrated for the first time by use of vibrational spectroscopy and wetting transparency, which revealed that trypsin was absorbed on GQDs’ surface through its cationic and hydrophilic residues. Our findings suggested that trypsin’s active sites were stabilized and protected by the GQDs, which were likely to be responsible for the high bioavailability of GQDs in enzymes.Conclusion: Our work demonstrates the efficacy of GQDs as an enzyme modulator with high specificity, and their great application potential in enzyme engineering as well as enzyme-based therapies. Keywords: graphene, enzyme, luminescence, bioavailability, surface energy
format	article
author	Tabish TA Pranjol MZI Karadag I Horsell DW Whatmore JL Zhang S
author_facet	Tabish TA Pranjol MZI Karadag I Horsell DW Whatmore JL Zhang S
author_sort	Tabish TA
title	Influence of luminescent graphene quantum dots on trypsin activity
title_short	Influence of luminescent graphene quantum dots on trypsin activity
title_full	Influence of luminescent graphene quantum dots on trypsin activity
title_fullStr	Influence of luminescent graphene quantum dots on trypsin activity
title_full_unstemmed	Influence of luminescent graphene quantum dots on trypsin activity
title_sort	influence of luminescent graphene quantum dots on trypsin activity
publisher	Dove Medical Press
publishDate	2018
url	https://doaj.org/article/05791b8a1362406c9a7fd10dad983a88
work_keys_str_mv	AT tabishta influenceofluminescentgraphenequantumdotsontrypsinactivity AT pranjolmzi influenceofluminescentgraphenequantumdotsontrypsinactivity AT karadagi influenceofluminescentgraphenequantumdotsontrypsinactivity AT horselldw influenceofluminescentgraphenequantumdotsontrypsinactivity AT whatmorejl influenceofluminescentgraphenequantumdotsontrypsinactivity AT zhangs influenceofluminescentgraphenequantumdotsontrypsinactivity
_version_	1718402509339361280

Influence of luminescent graphene quantum dots on trypsin activity

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