Enhanced methanol production in plants provides broad spectrum insect resistance.

Plants naturally emit methanol as volatile organic compound. Methanol is toxic to insect pests; but the quantity produced by most of the plants is not enough to protect them against invading insect pests. In the present study, we demonstrated that the over-expression of pectin methylesterase, derive...

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Autores principales:	Sameer Dixit, Santosh Kumar Upadhyay, Harpal Singh, Om Prakash Sidhu, Praveen Chandra Verma, Chandrashekar K
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2013
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/0579230e23384ea6850c874c0fbccc62
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spelling	oai:doaj.org-article:0579230e23384ea6850c874c0fbccc622021-11-18T08:48:16ZEnhanced methanol production in plants provides broad spectrum insect resistance.1932-620310.1371/journal.pone.0079664https://doaj.org/article/0579230e23384ea6850c874c0fbccc622013-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/24223989/?tool=EBIhttps://doaj.org/toc/1932-6203Plants naturally emit methanol as volatile organic compound. Methanol is toxic to insect pests; but the quantity produced by most of the plants is not enough to protect them against invading insect pests. In the present study, we demonstrated that the over-expression of pectin methylesterase, derived from Arabidopsis thaliana and Aspergillus niger, in transgenic tobacco plants enhances methanol production and resistance to polyphagous insect pests. Methanol content in the leaves of transgenic plants was measured using proton nuclear spectroscopy (1H NMR) and spectra showed up to 16 fold higher methanol as compared to control wild type (WT) plants. A maximum of 100 and 85% mortality in chewing insects Helicoverpa armigera and Spodoptera litura larvae was observed, respectively when fed on transgenic plants leaves. The surviving larvae showed less feeding, severe growth retardation and could not develop into pupae. In-planta bioassay on transgenic lines showed up to 99 and 75% reduction in the population multiplication of plant sap sucking pests Myzus persicae (aphid) and Bemisia tabaci (whitefly), respectively. Most of the phenotypic characters of transgenic plants were similar to WT plants. Confocal microscopy showed no deformities in cellular integrity, structure and density of stomata and trichomes of transgenic plants compared to WT. Pollen germination and tube formation was also not affected in transgenic plants. Cell wall enzyme transcript levels were comparable with WT. This study demonstrated for the first time that methanol emission can be utilized for imparting broad range insect resistance in plants.Sameer DixitSantosh Kumar UpadhyayHarpal SinghOm Prakash SidhuPraveen Chandra VermaChandrashekar KPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 8, Iss 11, p e79664 (2013)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Sameer Dixit Santosh Kumar Upadhyay Harpal Singh Om Prakash Sidhu Praveen Chandra Verma Chandrashekar K Enhanced methanol production in plants provides broad spectrum insect resistance.
description	Plants naturally emit methanol as volatile organic compound. Methanol is toxic to insect pests; but the quantity produced by most of the plants is not enough to protect them against invading insect pests. In the present study, we demonstrated that the over-expression of pectin methylesterase, derived from Arabidopsis thaliana and Aspergillus niger, in transgenic tobacco plants enhances methanol production and resistance to polyphagous insect pests. Methanol content in the leaves of transgenic plants was measured using proton nuclear spectroscopy (1H NMR) and spectra showed up to 16 fold higher methanol as compared to control wild type (WT) plants. A maximum of 100 and 85% mortality in chewing insects Helicoverpa armigera and Spodoptera litura larvae was observed, respectively when fed on transgenic plants leaves. The surviving larvae showed less feeding, severe growth retardation and could not develop into pupae. In-planta bioassay on transgenic lines showed up to 99 and 75% reduction in the population multiplication of plant sap sucking pests Myzus persicae (aphid) and Bemisia tabaci (whitefly), respectively. Most of the phenotypic characters of transgenic plants were similar to WT plants. Confocal microscopy showed no deformities in cellular integrity, structure and density of stomata and trichomes of transgenic plants compared to WT. Pollen germination and tube formation was also not affected in transgenic plants. Cell wall enzyme transcript levels were comparable with WT. This study demonstrated for the first time that methanol emission can be utilized for imparting broad range insect resistance in plants.
format	article
author	Sameer Dixit Santosh Kumar Upadhyay Harpal Singh Om Prakash Sidhu Praveen Chandra Verma Chandrashekar K
author_facet	Sameer Dixit Santosh Kumar Upadhyay Harpal Singh Om Prakash Sidhu Praveen Chandra Verma Chandrashekar K
author_sort	Sameer Dixit
title	Enhanced methanol production in plants provides broad spectrum insect resistance.
title_short	Enhanced methanol production in plants provides broad spectrum insect resistance.
title_full	Enhanced methanol production in plants provides broad spectrum insect resistance.
title_fullStr	Enhanced methanol production in plants provides broad spectrum insect resistance.
title_full_unstemmed	Enhanced methanol production in plants provides broad spectrum insect resistance.
title_sort	enhanced methanol production in plants provides broad spectrum insect resistance.
publisher	Public Library of Science (PLoS)
publishDate	2013
url	https://doaj.org/article/0579230e23384ea6850c874c0fbccc62
work_keys_str_mv	AT sameerdixit enhancedmethanolproductioninplantsprovidesbroadspectruminsectresistance AT santoshkumarupadhyay enhancedmethanolproductioninplantsprovidesbroadspectruminsectresistance AT harpalsingh enhancedmethanolproductioninplantsprovidesbroadspectruminsectresistance AT omprakashsidhu enhancedmethanolproductioninplantsprovidesbroadspectruminsectresistance AT praveenchandraverma enhancedmethanolproductioninplantsprovidesbroadspectruminsectresistance AT chandrashekark enhancedmethanolproductioninplantsprovidesbroadspectruminsectresistance
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Enhanced methanol production in plants provides broad spectrum insect resistance.

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