EFFECT OF MICRORNA-138 ON EPITHELIAL-MESENCHYMAL TRANSITION AND INVASION OF BREAST CANCER CELLS BY TARGETING SEMAPHORIN 4C

In view of the role of miR-138 in cancer cells, we predicted the target of miR-138 and its targeting to SEMA4C by bioinformatics software and luciferase experiment. The expression levels of miR-138 in human normal breast epithelial cells and two kinds of BC cells were compared, and the transfection...

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Autores principales:	HuiJuan Liu, Hui Ye, Xinzheng Li
Formato:	article
Lenguaje:	EN
Publicado:	Taylor & Francis Group 2021
Materias:	breast cancer mir-138 semaphorin 4c epithelial mesenchymal transition invasion Biotechnology TP248.13-248.65
Acceso en línea:	https://doaj.org/article/057c58bd534b4b82bc58b2d0ed8d0a3a
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id	oai:doaj.org-article:057c58bd534b4b82bc58b2d0ed8d0a3a
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spelling	oai:doaj.org-article:057c58bd534b4b82bc58b2d0ed8d0a3a2021-11-11T14:23:43ZEFFECT OF MICRORNA-138 ON EPITHELIAL-MESENCHYMAL TRANSITION AND INVASION OF BREAST CANCER CELLS BY TARGETING SEMAPHORIN 4C2165-59792165-598710.1080/21655979.2021.2000733https://doaj.org/article/057c58bd534b4b82bc58b2d0ed8d0a3a2021-11-01T00:00:00Zhttp://dx.doi.org/10.1080/21655979.2021.2000733https://doaj.org/toc/2165-5979https://doaj.org/toc/2165-5987In view of the role of miR-138 in cancer cells, we predicted the target of miR-138 and its targeting to SEMA4C by bioinformatics software and luciferase experiment. The expression levels of miR-138 in human normal breast epithelial cells and two kinds of BC cells were compared, and the transfection cells were selected. MiR-138 mimetic negative control (miR-NC), miR-138 mimic and miR-138 inhibitor were designed for cell transfection. The results showed that the expression level of miR-138 in MCF-7 cells was the lowest. The up regulation of miR-138 would lead to the high expression of E-cad and the low expression of N-cad, vim and SEMA4C, and the vitality and invasion of BC cells would decrease. The down regulation of miR-138 would lead to the low expression of E-cad and the high expression of N-cad, vim and SEMA4C, and the vitality and invasion of BC cells would increase. miR-138 targeted regulation of SEMA4C can promote the expression of N-cad, inhibit the expression of E-cad, vim and SEMA4C, reverse the EMT of BC cells, and inhibit the activity and invasion of BC cells. MiR-138 has clinical potential as a tumor marker of BC.HuiJuan LiuHui YeXinzheng LiTaylor & Francis Grouparticlebreast cancermir-138semaphorin 4cepithelial mesenchymal transitioninvasionBiotechnologyTP248.13-248.65ENBioengineered, Vol 0, Iss 0 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	breast cancer mir-138 semaphorin 4c epithelial mesenchymal transition invasion Biotechnology TP248.13-248.65
spellingShingle	breast cancer mir-138 semaphorin 4c epithelial mesenchymal transition invasion Biotechnology TP248.13-248.65 HuiJuan Liu Hui Ye Xinzheng Li EFFECT OF MICRORNA-138 ON EPITHELIAL-MESENCHYMAL TRANSITION AND INVASION OF BREAST CANCER CELLS BY TARGETING SEMAPHORIN 4C
description	In view of the role of miR-138 in cancer cells, we predicted the target of miR-138 and its targeting to SEMA4C by bioinformatics software and luciferase experiment. The expression levels of miR-138 in human normal breast epithelial cells and two kinds of BC cells were compared, and the transfection cells were selected. MiR-138 mimetic negative control (miR-NC), miR-138 mimic and miR-138 inhibitor were designed for cell transfection. The results showed that the expression level of miR-138 in MCF-7 cells was the lowest. The up regulation of miR-138 would lead to the high expression of E-cad and the low expression of N-cad, vim and SEMA4C, and the vitality and invasion of BC cells would decrease. The down regulation of miR-138 would lead to the low expression of E-cad and the high expression of N-cad, vim and SEMA4C, and the vitality and invasion of BC cells would increase. miR-138 targeted regulation of SEMA4C can promote the expression of N-cad, inhibit the expression of E-cad, vim and SEMA4C, reverse the EMT of BC cells, and inhibit the activity and invasion of BC cells. MiR-138 has clinical potential as a tumor marker of BC.
format	article
author	HuiJuan Liu Hui Ye Xinzheng Li
author_facet	HuiJuan Liu Hui Ye Xinzheng Li
author_sort	HuiJuan Liu
title	EFFECT OF MICRORNA-138 ON EPITHELIAL-MESENCHYMAL TRANSITION AND INVASION OF BREAST CANCER CELLS BY TARGETING SEMAPHORIN 4C
title_short	EFFECT OF MICRORNA-138 ON EPITHELIAL-MESENCHYMAL TRANSITION AND INVASION OF BREAST CANCER CELLS BY TARGETING SEMAPHORIN 4C
title_full	EFFECT OF MICRORNA-138 ON EPITHELIAL-MESENCHYMAL TRANSITION AND INVASION OF BREAST CANCER CELLS BY TARGETING SEMAPHORIN 4C
title_fullStr	EFFECT OF MICRORNA-138 ON EPITHELIAL-MESENCHYMAL TRANSITION AND INVASION OF BREAST CANCER CELLS BY TARGETING SEMAPHORIN 4C
title_full_unstemmed	EFFECT OF MICRORNA-138 ON EPITHELIAL-MESENCHYMAL TRANSITION AND INVASION OF BREAST CANCER CELLS BY TARGETING SEMAPHORIN 4C
title_sort	effect of microrna-138 on epithelial-mesenchymal transition and invasion of breast cancer cells by targeting semaphorin 4c
publisher	Taylor & Francis Group
publishDate	2021
url	https://doaj.org/article/057c58bd534b4b82bc58b2d0ed8d0a3a
work_keys_str_mv	AT huijuanliu effectofmicrorna138onepithelialmesenchymaltransitionandinvasionofbreastcancercellsbytargetingsemaphorin4c AT huiye effectofmicrorna138onepithelialmesenchymaltransitionandinvasionofbreastcancercellsbytargetingsemaphorin4c AT xinzhengli effectofmicrorna138onepithelialmesenchymaltransitionandinvasionofbreastcancercellsbytargetingsemaphorin4c
_version_	1718438910244159488

EFFECT OF MICRORNA-138 ON EPITHELIAL-MESENCHYMAL TRANSITION AND INVASION OF BREAST CANCER CELLS BY TARGETING SEMAPHORIN 4C

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