In Vitro Genotoxicity Assessment from the <i>Glycyrrhiza</i> New Variety Extract

The various species that comprise the genus <i>Glycyrrhiza</i> (Licorice) have long been used as oriental herbal medicines in Asian countries. Wongam (WG), which is a new variety of <i>Glycyrrhiza</i>, was developed in Korea to overcome the limitations of low productivity, en...

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Autores principales: Young-Jae Song, Dong-Gu Kim, Jeonghoon Lee, Wonnam Kim, Hyo-Jin An, Jong-Hyun Lee, Yong-Deok Jeon, Jaeki Chang, Sa-Haeng Kang, Ju-Ryoun Soh, Jong-Sik Jin
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Publicado: MDPI AG 2021
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Acceso en línea:https://doaj.org/article/057f9d0920d04e4d9059816d1e32cd77
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spelling oai:doaj.org-article:057f9d0920d04e4d9059816d1e32cd772021-11-11T15:17:42ZIn Vitro Genotoxicity Assessment from the <i>Glycyrrhiza</i> New Variety Extract10.3390/app1121102572076-3417https://doaj.org/article/057f9d0920d04e4d9059816d1e32cd772021-11-01T00:00:00Zhttps://www.mdpi.com/2076-3417/11/21/10257https://doaj.org/toc/2076-3417The various species that comprise the genus <i>Glycyrrhiza</i> (Licorice) have long been used as oriental herbal medicines in Asian countries. Wongam (WG), which is a new variety of <i>Glycyrrhiza</i>, was developed in Korea to overcome the limitations of low productivity, environmental restrictions, and an insufficient presence of glycyrrhizic acid and liquiritigenin. In this study, we evaluated WG extract’s genotoxicity through an in vitro bacterial reverse mutation (AMES) test, an in vitro chromosome aberration test, and an in vivo mouse bone marrow micronucleus test. In the AMES test, WG extract at concentrations of up to 5000 µg/plate showed no genotoxicity regardless of S9 mix. No chromosome aberrations appeared after 6 h in 1400 µg/mL WG extract regardless of S9 mix or in 1100 µg/mL WG extract after 24 h without S9 mix. Nor was there a significant increase in the number of micronucleated polychromatic erythrocytes to total erythrocytes up to 5000 mg/kg/day for 2 days detected in the micronucleus test. These results confirm that WG extract is safe for use as an herbal medicine, as it precipitates no detectable genotoxic effects.Young-Jae SongDong-Gu KimJeonghoon LeeWonnam KimHyo-Jin AnJong-Hyun LeeYong-Deok JeonJaeki ChangSa-Haeng KangJu-Ryoun SohJong-Sik JinMDPI AGarticle<i>Glycyrrhiza</i>wongamgenotoxicitybacterial reverse mutation testchromosome aberration testmicronucleus testTechnologyTEngineering (General). Civil engineering (General)TA1-2040Biology (General)QH301-705.5PhysicsQC1-999ChemistryQD1-999ENApplied Sciences, Vol 11, Iss 10257, p 10257 (2021)
institution DOAJ
collection DOAJ
language EN
topic <i>Glycyrrhiza</i>
wongam
genotoxicity
bacterial reverse mutation test
chromosome aberration test
micronucleus test
Technology
T
Engineering (General). Civil engineering (General)
TA1-2040
Biology (General)
QH301-705.5
Physics
QC1-999
Chemistry
QD1-999
spellingShingle <i>Glycyrrhiza</i>
wongam
genotoxicity
bacterial reverse mutation test
chromosome aberration test
micronucleus test
Technology
T
Engineering (General). Civil engineering (General)
TA1-2040
Biology (General)
QH301-705.5
Physics
QC1-999
Chemistry
QD1-999
Young-Jae Song
Dong-Gu Kim
Jeonghoon Lee
Wonnam Kim
Hyo-Jin An
Jong-Hyun Lee
Yong-Deok Jeon
Jaeki Chang
Sa-Haeng Kang
Ju-Ryoun Soh
Jong-Sik Jin
In Vitro Genotoxicity Assessment from the <i>Glycyrrhiza</i> New Variety Extract
description The various species that comprise the genus <i>Glycyrrhiza</i> (Licorice) have long been used as oriental herbal medicines in Asian countries. Wongam (WG), which is a new variety of <i>Glycyrrhiza</i>, was developed in Korea to overcome the limitations of low productivity, environmental restrictions, and an insufficient presence of glycyrrhizic acid and liquiritigenin. In this study, we evaluated WG extract’s genotoxicity through an in vitro bacterial reverse mutation (AMES) test, an in vitro chromosome aberration test, and an in vivo mouse bone marrow micronucleus test. In the AMES test, WG extract at concentrations of up to 5000 µg/plate showed no genotoxicity regardless of S9 mix. No chromosome aberrations appeared after 6 h in 1400 µg/mL WG extract regardless of S9 mix or in 1100 µg/mL WG extract after 24 h without S9 mix. Nor was there a significant increase in the number of micronucleated polychromatic erythrocytes to total erythrocytes up to 5000 mg/kg/day for 2 days detected in the micronucleus test. These results confirm that WG extract is safe for use as an herbal medicine, as it precipitates no detectable genotoxic effects.
format article
author Young-Jae Song
Dong-Gu Kim
Jeonghoon Lee
Wonnam Kim
Hyo-Jin An
Jong-Hyun Lee
Yong-Deok Jeon
Jaeki Chang
Sa-Haeng Kang
Ju-Ryoun Soh
Jong-Sik Jin
author_facet Young-Jae Song
Dong-Gu Kim
Jeonghoon Lee
Wonnam Kim
Hyo-Jin An
Jong-Hyun Lee
Yong-Deok Jeon
Jaeki Chang
Sa-Haeng Kang
Ju-Ryoun Soh
Jong-Sik Jin
author_sort Young-Jae Song
title In Vitro Genotoxicity Assessment from the <i>Glycyrrhiza</i> New Variety Extract
title_short In Vitro Genotoxicity Assessment from the <i>Glycyrrhiza</i> New Variety Extract
title_full In Vitro Genotoxicity Assessment from the <i>Glycyrrhiza</i> New Variety Extract
title_fullStr In Vitro Genotoxicity Assessment from the <i>Glycyrrhiza</i> New Variety Extract
title_full_unstemmed In Vitro Genotoxicity Assessment from the <i>Glycyrrhiza</i> New Variety Extract
title_sort in vitro genotoxicity assessment from the <i>glycyrrhiza</i> new variety extract
publisher MDPI AG
publishDate 2021
url https://doaj.org/article/057f9d0920d04e4d9059816d1e32cd77
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