Conserved Role for Biofilm Matrix Polysaccharides in <italic toggle="yes">Candida auris</italic> Drug Resistance

ABSTRACT Candida auris has emerged as an outbreak pathogen associated with high mortality. Biofilm formation and linked drug resistance are common among Candida species. Drug sequestration by the biofilm matrix accounts for much of the antifungal tolerance. In this study, we examine the biofilm matr...

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Autores principales:	E. G. Dominguez, R. Zarnowski, H. L. Choy, M. Zhao, H. Sanchez, Jeniel E. Nett, D. R. Andes
Formato:	article
Lenguaje:	EN
Publicado:	American Society for Microbiology 2019
Materias:	Candida auris biofilm matrix resistance Microbiology QR1-502
Acceso en línea:	https://doaj.org/article/058433b5f58a4203b22303e037204994
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spelling	oai:doaj.org-article:058433b5f58a4203b22303e0372049942021-11-15T15:22:04ZConserved Role for Biofilm Matrix Polysaccharides in <italic toggle="yes">Candida auris</italic> Drug Resistance10.1128/mSphereDirect.00680-182379-5042https://doaj.org/article/058433b5f58a4203b22303e0372049942019-02-01T00:00:00Zhttps://journals.asm.org/doi/10.1128/mSphereDirect.00680-18https://doaj.org/toc/2379-5042ABSTRACT Candida auris has emerged as an outbreak pathogen associated with high mortality. Biofilm formation and linked drug resistance are common among Candida species. Drug sequestration by the biofilm matrix accounts for much of the antifungal tolerance. In this study, we examine the biofilm matrix composition and function for a diverse set of C. auris isolates. We show that matrix sequesters nearly 70% of the available triazole antifungal. Like the biofilms formed by other Candida spp., we find that the matrix of C. auris is rich in mannan-glucan polysaccharides and demonstrate that their hydrolysis reduces drug tolerance. This biofilm matrix resistance mechanism appears conserved among Candida species, including C. auris. IMPORTANCE Candida auris is an emerging fungal threat linked to poor patient outcomes. The factors responsible for this apparent increase in pathogenicity remain largely unknown. Biofilm formation has been suggested as an important factor for persistence of this organism in patients and the environment. Our findings reveal one mechanism utilized by C. auris to evade the effect of triazole antifungal therapy during biofilm growth. The conservation of the protective biofilm matrix among Candida spp. suggests that is a promising pan-fungal Candida biofilm drug target.E. G. DominguezR. ZarnowskiH. L. ChoyM. ZhaoH. SanchezJeniel E. NettD. R. AndesAmerican Society for MicrobiologyarticleCandida aurisbiofilmmatrixresistanceMicrobiologyQR1-502ENmSphere, Vol 4, Iss 1 (2019)
institution	DOAJ
collection	DOAJ
language	EN
topic	Candida auris biofilm matrix resistance Microbiology QR1-502
spellingShingle	Candida auris biofilm matrix resistance Microbiology QR1-502 E. G. Dominguez R. Zarnowski H. L. Choy M. Zhao H. Sanchez Jeniel E. Nett D. R. Andes Conserved Role for Biofilm Matrix Polysaccharides in <italic toggle="yes">Candida auris</italic> Drug Resistance
description	ABSTRACT Candida auris has emerged as an outbreak pathogen associated with high mortality. Biofilm formation and linked drug resistance are common among Candida species. Drug sequestration by the biofilm matrix accounts for much of the antifungal tolerance. In this study, we examine the biofilm matrix composition and function for a diverse set of C. auris isolates. We show that matrix sequesters nearly 70% of the available triazole antifungal. Like the biofilms formed by other Candida spp., we find that the matrix of C. auris is rich in mannan-glucan polysaccharides and demonstrate that their hydrolysis reduces drug tolerance. This biofilm matrix resistance mechanism appears conserved among Candida species, including C. auris. IMPORTANCE Candida auris is an emerging fungal threat linked to poor patient outcomes. The factors responsible for this apparent increase in pathogenicity remain largely unknown. Biofilm formation has been suggested as an important factor for persistence of this organism in patients and the environment. Our findings reveal one mechanism utilized by C. auris to evade the effect of triazole antifungal therapy during biofilm growth. The conservation of the protective biofilm matrix among Candida spp. suggests that is a promising pan-fungal Candida biofilm drug target.
format	article
author	E. G. Dominguez R. Zarnowski H. L. Choy M. Zhao H. Sanchez Jeniel E. Nett D. R. Andes
author_facet	E. G. Dominguez R. Zarnowski H. L. Choy M. Zhao H. Sanchez Jeniel E. Nett D. R. Andes
author_sort	E. G. Dominguez
title	Conserved Role for Biofilm Matrix Polysaccharides in <italic toggle="yes">Candida auris</italic> Drug Resistance
title_short	Conserved Role for Biofilm Matrix Polysaccharides in <italic toggle="yes">Candida auris</italic> Drug Resistance
title_full	Conserved Role for Biofilm Matrix Polysaccharides in <italic toggle="yes">Candida auris</italic> Drug Resistance
title_fullStr	Conserved Role for Biofilm Matrix Polysaccharides in <italic toggle="yes">Candida auris</italic> Drug Resistance
title_full_unstemmed	Conserved Role for Biofilm Matrix Polysaccharides in <italic toggle="yes">Candida auris</italic> Drug Resistance
title_sort	conserved role for biofilm matrix polysaccharides in <italic toggle="yes">candida auris</italic> drug resistance
publisher	American Society for Microbiology
publishDate	2019
url	https://doaj.org/article/058433b5f58a4203b22303e037204994
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Conserved Role for Biofilm Matrix Polysaccharides in <italic toggle="yes">Candida auris</italic> Drug Resistance

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