Real-time, selective, and low-cost detection of trace level SARS-CoV-2 spike-protein for cold-chain food quarantine

Abstract Due to the friendly temperature for virus survival, SARS-CoV-2 is frequently found in cold-chain foods, posing a serious threat to public health. Utilizing an interdigitated microelectrode chip modified with an antibody probe and integrating dielectrophoresis enrichment with interfacial cap...

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Autores principales:	Jian Zhang, Xin Fang, Yu Mao, Haochen Qi, Jayne Wu, Xiaoru Liu, Fangshuo You, Wenci Zhao, Ying Chen, Lei Zheng
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Nutrition. Foods and food supply TX341-641 Food processing and manufacture TP368-456
Acceso en línea:	https://doaj.org/article/05846be512b44d359ef373f4a0c38e27
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spelling	oai:doaj.org-article:05846be512b44d359ef373f4a0c38e272021-12-02T18:24:51ZReal-time, selective, and low-cost detection of trace level SARS-CoV-2 spike-protein for cold-chain food quarantine10.1038/s41538-021-00094-32396-8370https://doaj.org/article/05846be512b44d359ef373f4a0c38e272021-06-01T00:00:00Zhttps://doi.org/10.1038/s41538-021-00094-3https://doaj.org/toc/2396-8370Abstract Due to the friendly temperature for virus survival, SARS-CoV-2 is frequently found in cold-chain foods, posing a serious threat to public health. Utilizing an interdigitated microelectrode chip modified with an antibody probe and integrating dielectrophoresis enrichment with interfacial capacitance sensing, a strategy is presented for the detection of trace level spike-protein from SARS-CoV-2. It achieves a limit of detection as low as 2.29 × 10−6 ng/mL in 20 s, with a wide linear range of 10−5–10−1 ng/mL and a selectivity of 234:1. The cost for a single test can be controlled to ~1 dollar. This strategy provides a competitive solution for real-time, sensitive, selective, and large-scale application in cold-chain food quarantine.Jian ZhangXin FangYu MaoHaochen QiJayne WuXiaoru LiuFangshuo YouWenci ZhaoYing ChenLei ZhengNature PortfolioarticleNutrition. Foods and food supplyTX341-641Food processing and manufactureTP368-456ENnpj Science of Food, Vol 5, Iss 1, Pp 1-6 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Nutrition. Foods and food supply TX341-641 Food processing and manufacture TP368-456
spellingShingle	Nutrition. Foods and food supply TX341-641 Food processing and manufacture TP368-456 Jian Zhang Xin Fang Yu Mao Haochen Qi Jayne Wu Xiaoru Liu Fangshuo You Wenci Zhao Ying Chen Lei Zheng Real-time, selective, and low-cost detection of trace level SARS-CoV-2 spike-protein for cold-chain food quarantine
description	Abstract Due to the friendly temperature for virus survival, SARS-CoV-2 is frequently found in cold-chain foods, posing a serious threat to public health. Utilizing an interdigitated microelectrode chip modified with an antibody probe and integrating dielectrophoresis enrichment with interfacial capacitance sensing, a strategy is presented for the detection of trace level spike-protein from SARS-CoV-2. It achieves a limit of detection as low as 2.29 × 10−6 ng/mL in 20 s, with a wide linear range of 10−5–10−1 ng/mL and a selectivity of 234:1. The cost for a single test can be controlled to ~1 dollar. This strategy provides a competitive solution for real-time, sensitive, selective, and large-scale application in cold-chain food quarantine.
format	article
author	Jian Zhang Xin Fang Yu Mao Haochen Qi Jayne Wu Xiaoru Liu Fangshuo You Wenci Zhao Ying Chen Lei Zheng
author_facet	Jian Zhang Xin Fang Yu Mao Haochen Qi Jayne Wu Xiaoru Liu Fangshuo You Wenci Zhao Ying Chen Lei Zheng
author_sort	Jian Zhang
title	Real-time, selective, and low-cost detection of trace level SARS-CoV-2 spike-protein for cold-chain food quarantine
title_short	Real-time, selective, and low-cost detection of trace level SARS-CoV-2 spike-protein for cold-chain food quarantine
title_full	Real-time, selective, and low-cost detection of trace level SARS-CoV-2 spike-protein for cold-chain food quarantine
title_fullStr	Real-time, selective, and low-cost detection of trace level SARS-CoV-2 spike-protein for cold-chain food quarantine
title_full_unstemmed	Real-time, selective, and low-cost detection of trace level SARS-CoV-2 spike-protein for cold-chain food quarantine
title_sort	real-time, selective, and low-cost detection of trace level sars-cov-2 spike-protein for cold-chain food quarantine
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/05846be512b44d359ef373f4a0c38e27
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Real-time, selective, and low-cost detection of trace level SARS-CoV-2 spike-protein for cold-chain food quarantine

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