Sodium alginate microencapsulation improves the short-term oral bioavailability of cannabidiol when administered with deoxycholic acid.
<h4>Background</h4>Cannabidiol (CBD) confers therapeutic effects in some neurological disorders via modulation of inflammatory, oxidative and cell-signalling pathways. However, CBD is lipophilic and highly photooxidative with low oral bioavailability in plasma and brain. In this study, w...
Guardado en:
Autores principales: | , , , , , , , , , |
---|---|
Formato: | article |
Lenguaje: | EN |
Publicado: |
Public Library of Science (PLoS)
2021
|
Materias: | |
Acceso en línea: | https://doaj.org/article/058676ea48c849268a27fc0338581c06 |
Etiquetas: |
Agregar Etiqueta
Sin Etiquetas, Sea el primero en etiquetar este registro!
|
id |
oai:doaj.org-article:058676ea48c849268a27fc0338581c06 |
---|---|
record_format |
dspace |
spelling |
oai:doaj.org-article:058676ea48c849268a27fc0338581c062021-11-25T06:23:31ZSodium alginate microencapsulation improves the short-term oral bioavailability of cannabidiol when administered with deoxycholic acid.1932-620310.1371/journal.pone.0243858https://doaj.org/article/058676ea48c849268a27fc0338581c062021-01-01T00:00:00Zhttps://doi.org/10.1371/journal.pone.0243858https://doaj.org/toc/1932-6203<h4>Background</h4>Cannabidiol (CBD) confers therapeutic effects in some neurological disorders via modulation of inflammatory, oxidative and cell-signalling pathways. However, CBD is lipophilic and highly photooxidative with low oral bioavailability in plasma and brain. In this study, we aimed to design and test a CBD microencapsulation method as a drug delivery strategy to improve the absorption of CBD. Additionally, we evaluated the brain uptake of CBD capsules when administered alongside capsules containing a permeation-modifying bile acid, deoxycholic acid (DCA).<h4>Methods</h4>Microcapsules containing either CBD or DCA were formed using the ionic gelation method with 1.5% sodium alginate formulations and 100 mM calcium chloride. C57BL/6J wild type mice randomly assigned to three treatment groups (3-4 mice per group) were administered CBD in the following preparations: 1) CBD capsules, 2) CBD capsules + DCA capsules and 3) naked CBD oil (control). To assess the short-term bioavailability of CBD, plasma and brain samples were collected at 0.3, 1 and 3 hours post administration and CBD levels were analysed with liquid chromatography mass spectrometer.<h4>Results</h4>We produced spherical capsules at 400 ± 50 μm in size. The CBD capsules were calculated to have a drug loading of 2% and an encapsulation efficiency of 23%. Mice that received CBD capsules + DCA capsules showed a 40% and 47% increase in CBD plasma concentration compared to mice on CBD capsules and naked CBD oil, respectively. Furthermore, the CBD capsules + DCA capsules group showed a 48% and 25% increase in CBD brain concentration compared to mice on CBD capsules and naked CBD oil, respectively. In mice treated with CBD capsules + DCA capsules, the brain CBD concentration peaked at 0.3 hours with a 300% increased availability compared to CBD capsules and naked CBD oil groups, which peaked at 1 hour after administration.<h4>Conclusions</h4>The microencapsulation method combined with a permeation enhancer, DCA increased the short-term bioavailability of CBD in plasma and brain.Maimuna MajimbiEmily BrookPeter GalettisEdward EdenHani Al-SalamiArmin MooranianHesham Al-SallamiVirginie LamJohn C L MamoRyusuke TakechiPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 16, Iss 6, p e0243858 (2021) |
institution |
DOAJ |
collection |
DOAJ |
language |
EN |
topic |
Medicine R Science Q |
spellingShingle |
Medicine R Science Q Maimuna Majimbi Emily Brook Peter Galettis Edward Eden Hani Al-Salami Armin Mooranian Hesham Al-Sallami Virginie Lam John C L Mamo Ryusuke Takechi Sodium alginate microencapsulation improves the short-term oral bioavailability of cannabidiol when administered with deoxycholic acid. |
description |
<h4>Background</h4>Cannabidiol (CBD) confers therapeutic effects in some neurological disorders via modulation of inflammatory, oxidative and cell-signalling pathways. However, CBD is lipophilic and highly photooxidative with low oral bioavailability in plasma and brain. In this study, we aimed to design and test a CBD microencapsulation method as a drug delivery strategy to improve the absorption of CBD. Additionally, we evaluated the brain uptake of CBD capsules when administered alongside capsules containing a permeation-modifying bile acid, deoxycholic acid (DCA).<h4>Methods</h4>Microcapsules containing either CBD or DCA were formed using the ionic gelation method with 1.5% sodium alginate formulations and 100 mM calcium chloride. C57BL/6J wild type mice randomly assigned to three treatment groups (3-4 mice per group) were administered CBD in the following preparations: 1) CBD capsules, 2) CBD capsules + DCA capsules and 3) naked CBD oil (control). To assess the short-term bioavailability of CBD, plasma and brain samples were collected at 0.3, 1 and 3 hours post administration and CBD levels were analysed with liquid chromatography mass spectrometer.<h4>Results</h4>We produced spherical capsules at 400 ± 50 μm in size. The CBD capsules were calculated to have a drug loading of 2% and an encapsulation efficiency of 23%. Mice that received CBD capsules + DCA capsules showed a 40% and 47% increase in CBD plasma concentration compared to mice on CBD capsules and naked CBD oil, respectively. Furthermore, the CBD capsules + DCA capsules group showed a 48% and 25% increase in CBD brain concentration compared to mice on CBD capsules and naked CBD oil, respectively. In mice treated with CBD capsules + DCA capsules, the brain CBD concentration peaked at 0.3 hours with a 300% increased availability compared to CBD capsules and naked CBD oil groups, which peaked at 1 hour after administration.<h4>Conclusions</h4>The microencapsulation method combined with a permeation enhancer, DCA increased the short-term bioavailability of CBD in plasma and brain. |
format |
article |
author |
Maimuna Majimbi Emily Brook Peter Galettis Edward Eden Hani Al-Salami Armin Mooranian Hesham Al-Sallami Virginie Lam John C L Mamo Ryusuke Takechi |
author_facet |
Maimuna Majimbi Emily Brook Peter Galettis Edward Eden Hani Al-Salami Armin Mooranian Hesham Al-Sallami Virginie Lam John C L Mamo Ryusuke Takechi |
author_sort |
Maimuna Majimbi |
title |
Sodium alginate microencapsulation improves the short-term oral bioavailability of cannabidiol when administered with deoxycholic acid. |
title_short |
Sodium alginate microencapsulation improves the short-term oral bioavailability of cannabidiol when administered with deoxycholic acid. |
title_full |
Sodium alginate microencapsulation improves the short-term oral bioavailability of cannabidiol when administered with deoxycholic acid. |
title_fullStr |
Sodium alginate microencapsulation improves the short-term oral bioavailability of cannabidiol when administered with deoxycholic acid. |
title_full_unstemmed |
Sodium alginate microencapsulation improves the short-term oral bioavailability of cannabidiol when administered with deoxycholic acid. |
title_sort |
sodium alginate microencapsulation improves the short-term oral bioavailability of cannabidiol when administered with deoxycholic acid. |
publisher |
Public Library of Science (PLoS) |
publishDate |
2021 |
url |
https://doaj.org/article/058676ea48c849268a27fc0338581c06 |
work_keys_str_mv |
AT maimunamajimbi sodiumalginatemicroencapsulationimprovestheshorttermoralbioavailabilityofcannabidiolwhenadministeredwithdeoxycholicacid AT emilybrook sodiumalginatemicroencapsulationimprovestheshorttermoralbioavailabilityofcannabidiolwhenadministeredwithdeoxycholicacid AT petergalettis sodiumalginatemicroencapsulationimprovestheshorttermoralbioavailabilityofcannabidiolwhenadministeredwithdeoxycholicacid AT edwardeden sodiumalginatemicroencapsulationimprovestheshorttermoralbioavailabilityofcannabidiolwhenadministeredwithdeoxycholicacid AT hanialsalami sodiumalginatemicroencapsulationimprovestheshorttermoralbioavailabilityofcannabidiolwhenadministeredwithdeoxycholicacid AT arminmooranian sodiumalginatemicroencapsulationimprovestheshorttermoralbioavailabilityofcannabidiolwhenadministeredwithdeoxycholicacid AT heshamalsallami sodiumalginatemicroencapsulationimprovestheshorttermoralbioavailabilityofcannabidiolwhenadministeredwithdeoxycholicacid AT virginielam sodiumalginatemicroencapsulationimprovestheshorttermoralbioavailabilityofcannabidiolwhenadministeredwithdeoxycholicacid AT johnclmamo sodiumalginatemicroencapsulationimprovestheshorttermoralbioavailabilityofcannabidiolwhenadministeredwithdeoxycholicacid AT ryusuketakechi sodiumalginatemicroencapsulationimprovestheshorttermoralbioavailabilityofcannabidiolwhenadministeredwithdeoxycholicacid |
_version_ |
1718413783322329088 |