Comparison of polymerase chain reaction and microscopy for the detection of Fasciola spp. in the fecal matter of domestic bovines in Kalasin Province, Thailand

Background and Aim: Fasciola spp. are important foodborne trematodes and waterborne zoonotic parasites that cause health problems and economic losses worldwide, including in Thailand. Fasciola spp. are usually detected by sedimentation or the formalin-ethyl acetate concentration technique (FECT) und...

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Autores principales:	Sirikanda Thanasuwan, Anupong Tankrathok
Formato:	article
Lenguaje:	EN
Publicado:	Veterinary World 2021
Materias:	28s rrna bovine fasciola gigantica kalasin province lampao dam polymerase chain reaction Animal culture SF1-1100 Veterinary medicine SF600-1100
Acceso en línea:	https://doaj.org/article/059125de3d81428c85b80650e2219557
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spelling	oai:doaj.org-article:059125de3d81428c85b80650e22195572021-11-10T11:12:19ZComparison of polymerase chain reaction and microscopy for the detection of Fasciola spp. in the fecal matter of domestic bovines in Kalasin Province, Thailand10.14202/vetworld.2021.2878-28820972-89882231-0916https://doaj.org/article/059125de3d81428c85b80650e22195572021-11-01T00:00:00Zhttp://www.veterinaryworld.org/Vol.14/November-2021/6.pdfhttps://doaj.org/toc/0972-8988https://doaj.org/toc/2231-0916Background and Aim: Fasciola spp. are important foodborne trematodes and waterborne zoonotic parasites that cause health problems and economic losses worldwide, including in Thailand. Fasciola spp. are usually detected by sedimentation or the formalin-ethyl acetate concentration technique (FECT) under microscopy, which is less specific and sensitive. Accurate detection is important to detect real incidence for protection against and elimination of fasciolosis in the area. This study aimed to determine the distribution of Fasciola spp. and compare the specificity and sensitivity of FECT under microscopy to that of polymerase chain reaction (PCR) in cattle feces. Materials and Methods: The study was conducted in Kalasin Province, Thailand. Feces of 46 cattle were investigated for infection with Fasciola spp. To detect infection, FECT under microscopy and PCR amplification of the 28S rRNA gene of Fasciola spp. were used to identify egg parasites. Results: Feces of 16 of 46 (34.78%) cattle were positive for Fasciola spp. using FECT under microscopy, whereas PCR showed that 67.39% (31 of 46) were positive for Fasciola spp. False-negative results were as high as 32.61% when diagnosed under microscopy. Conclusion: This study confirmed the infection of cattle with Fasciola spp. in Kalasin Province, indicating that PCR demonstrated higher sensitivity and specificity when diagnosing infection. FECT under microscopy can still be used as a primary and traditional method for diagnosis. However, relapse cases of Fasciola spp. and Paramphistomum spp. should be diagnosed by microscopy combined with PCR. This is the first report on the molecular distribution of fecal samples in cattle in Kalasin Province.Sirikanda ThanasuwanAnupong TankrathokVeterinary Worldarticle28s rrnabovinefasciola giganticakalasin provincelampao dampolymerase chain reactionAnimal cultureSF1-1100Veterinary medicineSF600-1100ENVeterinary World, Vol 14, Iss 11, Pp 2878-2882 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	28s rrna bovine fasciola gigantica kalasin province lampao dam polymerase chain reaction Animal culture SF1-1100 Veterinary medicine SF600-1100
spellingShingle	28s rrna bovine fasciola gigantica kalasin province lampao dam polymerase chain reaction Animal culture SF1-1100 Veterinary medicine SF600-1100 Sirikanda Thanasuwan Anupong Tankrathok Comparison of polymerase chain reaction and microscopy for the detection of Fasciola spp. in the fecal matter of domestic bovines in Kalasin Province, Thailand
description	Background and Aim: Fasciola spp. are important foodborne trematodes and waterborne zoonotic parasites that cause health problems and economic losses worldwide, including in Thailand. Fasciola spp. are usually detected by sedimentation or the formalin-ethyl acetate concentration technique (FECT) under microscopy, which is less specific and sensitive. Accurate detection is important to detect real incidence for protection against and elimination of fasciolosis in the area. This study aimed to determine the distribution of Fasciola spp. and compare the specificity and sensitivity of FECT under microscopy to that of polymerase chain reaction (PCR) in cattle feces. Materials and Methods: The study was conducted in Kalasin Province, Thailand. Feces of 46 cattle were investigated for infection with Fasciola spp. To detect infection, FECT under microscopy and PCR amplification of the 28S rRNA gene of Fasciola spp. were used to identify egg parasites. Results: Feces of 16 of 46 (34.78%) cattle were positive for Fasciola spp. using FECT under microscopy, whereas PCR showed that 67.39% (31 of 46) were positive for Fasciola spp. False-negative results were as high as 32.61% when diagnosed under microscopy. Conclusion: This study confirmed the infection of cattle with Fasciola spp. in Kalasin Province, indicating that PCR demonstrated higher sensitivity and specificity when diagnosing infection. FECT under microscopy can still be used as a primary and traditional method for diagnosis. However, relapse cases of Fasciola spp. and Paramphistomum spp. should be diagnosed by microscopy combined with PCR. This is the first report on the molecular distribution of fecal samples in cattle in Kalasin Province.
format	article
author	Sirikanda Thanasuwan Anupong Tankrathok
author_facet	Sirikanda Thanasuwan Anupong Tankrathok
author_sort	Sirikanda Thanasuwan
title	Comparison of polymerase chain reaction and microscopy for the detection of Fasciola spp. in the fecal matter of domestic bovines in Kalasin Province, Thailand
title_short	Comparison of polymerase chain reaction and microscopy for the detection of Fasciola spp. in the fecal matter of domestic bovines in Kalasin Province, Thailand
title_full	Comparison of polymerase chain reaction and microscopy for the detection of Fasciola spp. in the fecal matter of domestic bovines in Kalasin Province, Thailand
title_fullStr	Comparison of polymerase chain reaction and microscopy for the detection of Fasciola spp. in the fecal matter of domestic bovines in Kalasin Province, Thailand
title_full_unstemmed	Comparison of polymerase chain reaction and microscopy for the detection of Fasciola spp. in the fecal matter of domestic bovines in Kalasin Province, Thailand
title_sort	comparison of polymerase chain reaction and microscopy for the detection of fasciola spp. in the fecal matter of domestic bovines in kalasin province, thailand
publisher	Veterinary World
publishDate	2021
url	https://doaj.org/article/059125de3d81428c85b80650e2219557
work_keys_str_mv	AT sirikandathanasuwan comparisonofpolymerasechainreactionandmicroscopyforthedetectionoffasciolasppinthefecalmatterofdomesticbovinesinkalasinprovincethailand AT anupongtankrathok comparisonofpolymerasechainreactionandmicroscopyforthedetectionoffasciolasppinthefecalmatterofdomesticbovinesinkalasinprovincethailand
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Comparison of polymerase chain reaction and microscopy for the detection of Fasciola spp. in the fecal matter of domestic bovines in Kalasin Province, Thailand

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