Etiology of granulomatous lobular mastitis based on metagenomic next-generation sequencing

Objectives: We aimed to comprehensively explore the etiology of granulomatous lobular mastitis (GLM) to optimize treatment programs.Methods: We collected 30 fresh mastitis samples for metagenomic next-generation sequencing, morphological observation, and analysis of the clinical information.Results:...

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Autores principales:	Jiaxin Bi, Zhixin Li, Xiao Lin, Fang Li, Hua Xu, Xuewen Yu, Lingyun Liu, Yingying Liang, Zhouwen Xu, Jiachuan Wang, Mumin Shao
Formato:	article
Lenguaje:	EN
Publicado:	Elsevier 2021
Materias:	granulomatous lobular mastitis pathogens pathology metagenomic next-generation sequencing Infectious and parasitic diseases RC109-216
Acceso en línea:	https://doaj.org/article/05964037860e49c0a383664f87f196f9
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spelling	oai:doaj.org-article:05964037860e49c0a383664f87f196f92021-11-14T04:31:15ZEtiology of granulomatous lobular mastitis based on metagenomic next-generation sequencing1201-971210.1016/j.ijid.2021.10.019https://doaj.org/article/05964037860e49c0a383664f87f196f92021-12-01T00:00:00Zhttp://www.sciencedirect.com/science/article/pii/S1201971221008109https://doaj.org/toc/1201-9712Objectives: We aimed to comprehensively explore the etiology of granulomatous lobular mastitis (GLM) to optimize treatment programs.Methods: We collected 30 fresh mastitis samples for metagenomic next-generation sequencing, morphological observation, and analysis of the clinical information.Results: Of the 30 samples, 25 were GLM; pathogens were detected in 17, these were: Corynebacterium kroppenstedtii (10 of 25, 40%); C. kroppenstedtii and Pseudomonas oleovorans (3 of 25, 12%); C. kroppenstedtii and human gammaherpesvirus 4 (1 of 25, 4%); Acinetobacter baumannii and C. kroppenstedtii (1 of 25, 4%); P. oleovorans (1 of 25, 4%); and Tepidiphilus thermophilus (1 of 25, 4%). Abnormal sex hormone levels (mainly prolactin) and/or autoimmune function were found in 12 of the 25 samples. Lipophilic antibiotics (rifampicin) were found to work effectively in patients with slow-healing wounds after surgery.Conclusions: The main pathogenic factor of GLM is C. kroppenstedtii infection, but other unusual pathogens (P. oleovorans, human gammaherpesvirus 4, A. baumannii, T. thermophilus) are likely to be closely related to GLM, particularly human gammaherpesvirus 4 (Epstein-Barr virus)-associated mastitis, which may be a new entity of mastitis. Abnormal levels of sex hormones and autoimmune function are also common causes. Therefore, lipophilic antibiotics (rifampicin) and prolactin inhibitors may be an effective treatment.Jiaxin BiZhixin LiXiao LinFang LiHua XuXuewen YuLingyun LiuYingying LiangZhouwen XuJiachuan WangMumin ShaoElsevierarticlegranulomatous lobular mastitispathogenspathologymetagenomic next-generation sequencingInfectious and parasitic diseasesRC109-216ENInternational Journal of Infectious Diseases, Vol 113, Iss , Pp 243-250 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	granulomatous lobular mastitis pathogens pathology metagenomic next-generation sequencing Infectious and parasitic diseases RC109-216
spellingShingle	granulomatous lobular mastitis pathogens pathology metagenomic next-generation sequencing Infectious and parasitic diseases RC109-216 Jiaxin Bi Zhixin Li Xiao Lin Fang Li Hua Xu Xuewen Yu Lingyun Liu Yingying Liang Zhouwen Xu Jiachuan Wang Mumin Shao Etiology of granulomatous lobular mastitis based on metagenomic next-generation sequencing
description	Objectives: We aimed to comprehensively explore the etiology of granulomatous lobular mastitis (GLM) to optimize treatment programs.Methods: We collected 30 fresh mastitis samples for metagenomic next-generation sequencing, morphological observation, and analysis of the clinical information.Results: Of the 30 samples, 25 were GLM; pathogens were detected in 17, these were: Corynebacterium kroppenstedtii (10 of 25, 40%); C. kroppenstedtii and Pseudomonas oleovorans (3 of 25, 12%); C. kroppenstedtii and human gammaherpesvirus 4 (1 of 25, 4%); Acinetobacter baumannii and C. kroppenstedtii (1 of 25, 4%); P. oleovorans (1 of 25, 4%); and Tepidiphilus thermophilus (1 of 25, 4%). Abnormal sex hormone levels (mainly prolactin) and/or autoimmune function were found in 12 of the 25 samples. Lipophilic antibiotics (rifampicin) were found to work effectively in patients with slow-healing wounds after surgery.Conclusions: The main pathogenic factor of GLM is C. kroppenstedtii infection, but other unusual pathogens (P. oleovorans, human gammaherpesvirus 4, A. baumannii, T. thermophilus) are likely to be closely related to GLM, particularly human gammaherpesvirus 4 (Epstein-Barr virus)-associated mastitis, which may be a new entity of mastitis. Abnormal levels of sex hormones and autoimmune function are also common causes. Therefore, lipophilic antibiotics (rifampicin) and prolactin inhibitors may be an effective treatment.
format	article
author	Jiaxin Bi Zhixin Li Xiao Lin Fang Li Hua Xu Xuewen Yu Lingyun Liu Yingying Liang Zhouwen Xu Jiachuan Wang Mumin Shao
author_facet	Jiaxin Bi Zhixin Li Xiao Lin Fang Li Hua Xu Xuewen Yu Lingyun Liu Yingying Liang Zhouwen Xu Jiachuan Wang Mumin Shao
author_sort	Jiaxin Bi
title	Etiology of granulomatous lobular mastitis based on metagenomic next-generation sequencing
title_short	Etiology of granulomatous lobular mastitis based on metagenomic next-generation sequencing
title_full	Etiology of granulomatous lobular mastitis based on metagenomic next-generation sequencing
title_fullStr	Etiology of granulomatous lobular mastitis based on metagenomic next-generation sequencing
title_full_unstemmed	Etiology of granulomatous lobular mastitis based on metagenomic next-generation sequencing
title_sort	etiology of granulomatous lobular mastitis based on metagenomic next-generation sequencing
publisher	Elsevier
publishDate	2021
url	https://doaj.org/article/05964037860e49c0a383664f87f196f9
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_version_	1718429938086838272

Etiology of granulomatous lobular mastitis based on metagenomic next-generation sequencing

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