Flotillins are involved in the polarization of primitive and mature hematopoietic cells.

<h4>Background</h4>Migration of mature and immature leukocytes in response to chemokines is not only essential during inflammation and host defense, but also during development of the hematopoietic system. Many molecules implicated in migratory polarity show uniform cellular distribution...

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Autores principales:	Lawrence Rajendran, Julia Beckmann, Astrid Magenau, Eva-Maria Boneberg, Katharina Gaus, Antonella Viola, Bernd Giebel, Harald Illges
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2009
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/0598033cae2b42d8b018ec160fe48b24
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spelling	oai:doaj.org-article:0598033cae2b42d8b018ec160fe48b242021-11-25T06:27:12ZFlotillins are involved in the polarization of primitive and mature hematopoietic cells.1932-620310.1371/journal.pone.0008290https://doaj.org/article/0598033cae2b42d8b018ec160fe48b242009-12-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/20027317/?tool=EBIhttps://doaj.org/toc/1932-6203<h4>Background</h4>Migration of mature and immature leukocytes in response to chemokines is not only essential during inflammation and host defense, but also during development of the hematopoietic system. Many molecules implicated in migratory polarity show uniform cellular distribution under non-activated conditions, but acquire a polarized localization upon exposure to migratory cues.<h4>Methodology/principal findings</h4>Here, we present evidence that raft-associated endocytic proteins (flotillins) are pre-assembled in lymphoid, myeloid and primitive hematopoietic cells and accumulate in the uropod during migration. Furthermore, flotillins display a polarized distribution during immunological synapse formation. Employing the membrane lipid-order sensitive probe Laurdan, we show that flotillin accumulation in the immunological synapse is concomittant with membrane ordering in these regions.<h4>Conclusions</h4>Together with the observation that flotillin polarization does not occur in other polarized cell types such as polarized epithelial cells, our results suggest a specific role for flotillins in hematopoietic cell polarization. Based on our results, we propose that in hematopoietic cells, flotillins provide intrinsic cues that govern segregation of certain microdomain-associated molecules during immune cell polarization.Lawrence RajendranJulia BeckmannAstrid MagenauEva-Maria BonebergKatharina GausAntonella ViolaBernd GiebelHarald IllgesPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 4, Iss 12, p e8290 (2009)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Lawrence Rajendran Julia Beckmann Astrid Magenau Eva-Maria Boneberg Katharina Gaus Antonella Viola Bernd Giebel Harald Illges Flotillins are involved in the polarization of primitive and mature hematopoietic cells.
description	<h4>Background</h4>Migration of mature and immature leukocytes in response to chemokines is not only essential during inflammation and host defense, but also during development of the hematopoietic system. Many molecules implicated in migratory polarity show uniform cellular distribution under non-activated conditions, but acquire a polarized localization upon exposure to migratory cues.<h4>Methodology/principal findings</h4>Here, we present evidence that raft-associated endocytic proteins (flotillins) are pre-assembled in lymphoid, myeloid and primitive hematopoietic cells and accumulate in the uropod during migration. Furthermore, flotillins display a polarized distribution during immunological synapse formation. Employing the membrane lipid-order sensitive probe Laurdan, we show that flotillin accumulation in the immunological synapse is concomittant with membrane ordering in these regions.<h4>Conclusions</h4>Together with the observation that flotillin polarization does not occur in other polarized cell types such as polarized epithelial cells, our results suggest a specific role for flotillins in hematopoietic cell polarization. Based on our results, we propose that in hematopoietic cells, flotillins provide intrinsic cues that govern segregation of certain microdomain-associated molecules during immune cell polarization.
format	article
author	Lawrence Rajendran Julia Beckmann Astrid Magenau Eva-Maria Boneberg Katharina Gaus Antonella Viola Bernd Giebel Harald Illges
author_facet	Lawrence Rajendran Julia Beckmann Astrid Magenau Eva-Maria Boneberg Katharina Gaus Antonella Viola Bernd Giebel Harald Illges
author_sort	Lawrence Rajendran
title	Flotillins are involved in the polarization of primitive and mature hematopoietic cells.
title_short	Flotillins are involved in the polarization of primitive and mature hematopoietic cells.
title_full	Flotillins are involved in the polarization of primitive and mature hematopoietic cells.
title_fullStr	Flotillins are involved in the polarization of primitive and mature hematopoietic cells.
title_full_unstemmed	Flotillins are involved in the polarization of primitive and mature hematopoietic cells.
title_sort	flotillins are involved in the polarization of primitive and mature hematopoietic cells.
publisher	Public Library of Science (PLoS)
publishDate	2009
url	https://doaj.org/article/0598033cae2b42d8b018ec160fe48b24
work_keys_str_mv	AT lawrencerajendran flotillinsareinvolvedinthepolarizationofprimitiveandmaturehematopoieticcells AT juliabeckmann flotillinsareinvolvedinthepolarizationofprimitiveandmaturehematopoieticcells AT astridmagenau flotillinsareinvolvedinthepolarizationofprimitiveandmaturehematopoieticcells AT evamariaboneberg flotillinsareinvolvedinthepolarizationofprimitiveandmaturehematopoieticcells AT katharinagaus flotillinsareinvolvedinthepolarizationofprimitiveandmaturehematopoieticcells AT antonellaviola flotillinsareinvolvedinthepolarizationofprimitiveandmaturehematopoieticcells AT berndgiebel flotillinsareinvolvedinthepolarizationofprimitiveandmaturehematopoieticcells AT haraldillges flotillinsareinvolvedinthepolarizationofprimitiveandmaturehematopoieticcells
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Flotillins are involved in the polarization of primitive and mature hematopoietic cells.

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