MicroRNA Expression Profiles in Gastric Carcinogenesis

Abstract Intestinal-type gastric carcinoma exhibits a multistep carcinogenic sequence from adenoma to carcinoma with a gradual increase in genomic alterations. But the roles of microRNAs (miRNA) in this multistage cascade are not fully explored. To identify differentially expressed miRNA (DEM) durin...

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Autores principales:	Jinha Hwang, Byung-Hoon Min, Jiryeon Jang, So Young Kang, Hyunsik Bae, Se Song Jang, Jong-Il Kim, Kyoung-Mee Kim
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2018
Materias:	Gastric Carcinogenesis Differentially Expressed miRNA (DEMs) Adenoma Samples Early Gastric Cancer (EGC) TCGA Datasets Medicine R Science Q
Acceso en línea:	https://doaj.org/article/05a30b1683cb4f82aa7a2c1e97084bec
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spelling	oai:doaj.org-article:05a30b1683cb4f82aa7a2c1e97084bec2021-12-02T11:41:03ZMicroRNA Expression Profiles in Gastric Carcinogenesis10.1038/s41598-018-32782-82045-2322https://doaj.org/article/05a30b1683cb4f82aa7a2c1e97084bec2018-09-01T00:00:00Zhttps://doi.org/10.1038/s41598-018-32782-8https://doaj.org/toc/2045-2322Abstract Intestinal-type gastric carcinoma exhibits a multistep carcinogenic sequence from adenoma to carcinoma with a gradual increase in genomic alterations. But the roles of microRNAs (miRNA) in this multistage cascade are not fully explored. To identify differentially expressed miRNA (DEM) during early gastric carcinogenesis, we performed miRNA microarray profiling with 24 gastric cancers and precursor lesions (7 early gastric cancer [EGC], 3 adenomas with high-grade dysplasia, 4 adenomas with low-grade dysplasia, and 10 adjacent normal tissues). Alterations in the expression of 132 miRNA were detected; these were categorized into three groups based on their expression patterns. Of these, 42 miRNAs were aberrantly expressed in EGC. Five miRNA (miR-26a, miR-375, miR-574-3p, miR-145, and miR-15b) showed decreased expression since adenoma. Expression of two miRNA, miR-200C and miR-29a, was down-regulated in EGCs compared to normal mucosa or adenomas. Six miRNA (miR-601, miR-107, miR-18a, miR-370, miR-300, and miR-96) showed increased expression in gastric cancer compared to normal or adenoma samples. Five representative miRNAs were further validated with RT-qPCR in independent 77 samples. Taken together, these results suggest that the dysregulated miRNA show alterations at the early stages of gastric tumorigenesis and may be used as a candidate biomarker.Jinha HwangByung-Hoon MinJiryeon JangSo Young KangHyunsik BaeSe Song JangJong-Il KimKyoung-Mee KimNature PortfolioarticleGastric CarcinogenesisDifferentially Expressed miRNA (DEMs)Adenoma SamplesEarly Gastric Cancer (EGC)TCGA DatasetsMedicineRScienceQENScientific Reports, Vol 8, Iss 1, Pp 1-8 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	Gastric Carcinogenesis Differentially Expressed miRNA (DEMs) Adenoma Samples Early Gastric Cancer (EGC) TCGA Datasets Medicine R Science Q
spellingShingle	Gastric Carcinogenesis Differentially Expressed miRNA (DEMs) Adenoma Samples Early Gastric Cancer (EGC) TCGA Datasets Medicine R Science Q Jinha Hwang Byung-Hoon Min Jiryeon Jang So Young Kang Hyunsik Bae Se Song Jang Jong-Il Kim Kyoung-Mee Kim MicroRNA Expression Profiles in Gastric Carcinogenesis
description	Abstract Intestinal-type gastric carcinoma exhibits a multistep carcinogenic sequence from adenoma to carcinoma with a gradual increase in genomic alterations. But the roles of microRNAs (miRNA) in this multistage cascade are not fully explored. To identify differentially expressed miRNA (DEM) during early gastric carcinogenesis, we performed miRNA microarray profiling with 24 gastric cancers and precursor lesions (7 early gastric cancer [EGC], 3 adenomas with high-grade dysplasia, 4 adenomas with low-grade dysplasia, and 10 adjacent normal tissues). Alterations in the expression of 132 miRNA were detected; these were categorized into three groups based on their expression patterns. Of these, 42 miRNAs were aberrantly expressed in EGC. Five miRNA (miR-26a, miR-375, miR-574-3p, miR-145, and miR-15b) showed decreased expression since adenoma. Expression of two miRNA, miR-200C and miR-29a, was down-regulated in EGCs compared to normal mucosa or adenomas. Six miRNA (miR-601, miR-107, miR-18a, miR-370, miR-300, and miR-96) showed increased expression in gastric cancer compared to normal or adenoma samples. Five representative miRNAs were further validated with RT-qPCR in independent 77 samples. Taken together, these results suggest that the dysregulated miRNA show alterations at the early stages of gastric tumorigenesis and may be used as a candidate biomarker.
format	article
author	Jinha Hwang Byung-Hoon Min Jiryeon Jang So Young Kang Hyunsik Bae Se Song Jang Jong-Il Kim Kyoung-Mee Kim
author_facet	Jinha Hwang Byung-Hoon Min Jiryeon Jang So Young Kang Hyunsik Bae Se Song Jang Jong-Il Kim Kyoung-Mee Kim
author_sort	Jinha Hwang
title	MicroRNA Expression Profiles in Gastric Carcinogenesis
title_short	MicroRNA Expression Profiles in Gastric Carcinogenesis
title_full	MicroRNA Expression Profiles in Gastric Carcinogenesis
title_fullStr	MicroRNA Expression Profiles in Gastric Carcinogenesis
title_full_unstemmed	MicroRNA Expression Profiles in Gastric Carcinogenesis
title_sort	microrna expression profiles in gastric carcinogenesis
publisher	Nature Portfolio
publishDate	2018
url	https://doaj.org/article/05a30b1683cb4f82aa7a2c1e97084bec
work_keys_str_mv	AT jinhahwang micrornaexpressionprofilesingastriccarcinogenesis AT byunghoonmin micrornaexpressionprofilesingastriccarcinogenesis AT jiryeonjang micrornaexpressionprofilesingastriccarcinogenesis AT soyoungkang micrornaexpressionprofilesingastriccarcinogenesis AT hyunsikbae micrornaexpressionprofilesingastriccarcinogenesis AT sesongjang micrornaexpressionprofilesingastriccarcinogenesis AT jongilkim micrornaexpressionprofilesingastriccarcinogenesis AT kyoungmeekim micrornaexpressionprofilesingastriccarcinogenesis
_version_	1718395441420173312

MicroRNA Expression Profiles in Gastric Carcinogenesis

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