Calcium dynamics in root cells of Arabidopsis thaliana visualized with selective plane illumination microscopy.

Selective Plane Illumination Microscopy (SPIM) is an imaging technique particularly suited for long term in-vivo analysis of transparent specimens, able to visualize small organs or entire organisms, at cellular and eventually even subcellular resolution. Here we report the application of SPIM in Ca...

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Autores principales:	Alex Costa, Alessia Candeo, Luca Fieramonti, Gianluca Valentini, Andrea Bassi
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2013
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/05aa325785764793af53f1b65c31a58f
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spelling	oai:doaj.org-article:05aa325785764793af53f1b65c31a58f2021-11-18T08:50:53ZCalcium dynamics in root cells of Arabidopsis thaliana visualized with selective plane illumination microscopy.1932-620310.1371/journal.pone.0075646https://doaj.org/article/05aa325785764793af53f1b65c31a58f2013-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/24146766/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203Selective Plane Illumination Microscopy (SPIM) is an imaging technique particularly suited for long term in-vivo analysis of transparent specimens, able to visualize small organs or entire organisms, at cellular and eventually even subcellular resolution. Here we report the application of SPIM in Calcium imaging based on Förster Resonance Energy Transfer (FRET). Transgenic Arabidopsis plants expressing the genetically encoded-FRET-based Ca(2+) probe Cameleon, in the cytosol or nucleus, were used to demonstrate that SPIM enables ratiometric fluorescence imaging at high spatial and temporal resolution, both at tissue and single cell level. The SPIM-FRET technique enabled us to follow nuclear and cytosolic Ca(2+) dynamics in Arabidopsis root tip cells, deep inside the organ, in response to different stimuli. A relevant physiological phenomenon, namely Ca(2+) signal percolation, predicted in previous studies, has been directly visualized.Alex CostaAlessia CandeoLuca FieramontiGianluca ValentiniAndrea BassiPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 8, Iss 10, p e75646 (2013)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Alex Costa Alessia Candeo Luca Fieramonti Gianluca Valentini Andrea Bassi Calcium dynamics in root cells of Arabidopsis thaliana visualized with selective plane illumination microscopy.
description	Selective Plane Illumination Microscopy (SPIM) is an imaging technique particularly suited for long term in-vivo analysis of transparent specimens, able to visualize small organs or entire organisms, at cellular and eventually even subcellular resolution. Here we report the application of SPIM in Calcium imaging based on Förster Resonance Energy Transfer (FRET). Transgenic Arabidopsis plants expressing the genetically encoded-FRET-based Ca(2+) probe Cameleon, in the cytosol or nucleus, were used to demonstrate that SPIM enables ratiometric fluorescence imaging at high spatial and temporal resolution, both at tissue and single cell level. The SPIM-FRET technique enabled us to follow nuclear and cytosolic Ca(2+) dynamics in Arabidopsis root tip cells, deep inside the organ, in response to different stimuli. A relevant physiological phenomenon, namely Ca(2+) signal percolation, predicted in previous studies, has been directly visualized.
format	article
author	Alex Costa Alessia Candeo Luca Fieramonti Gianluca Valentini Andrea Bassi
author_facet	Alex Costa Alessia Candeo Luca Fieramonti Gianluca Valentini Andrea Bassi
author_sort	Alex Costa
title	Calcium dynamics in root cells of Arabidopsis thaliana visualized with selective plane illumination microscopy.
title_short	Calcium dynamics in root cells of Arabidopsis thaliana visualized with selective plane illumination microscopy.
title_full	Calcium dynamics in root cells of Arabidopsis thaliana visualized with selective plane illumination microscopy.
title_fullStr	Calcium dynamics in root cells of Arabidopsis thaliana visualized with selective plane illumination microscopy.
title_full_unstemmed	Calcium dynamics in root cells of Arabidopsis thaliana visualized with selective plane illumination microscopy.
title_sort	calcium dynamics in root cells of arabidopsis thaliana visualized with selective plane illumination microscopy.
publisher	Public Library of Science (PLoS)
publishDate	2013
url	https://doaj.org/article/05aa325785764793af53f1b65c31a58f
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Calcium dynamics in root cells of Arabidopsis thaliana visualized with selective plane illumination microscopy.

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