Hyaluronan-Based Gel Promotes Human Dental Pulp Stem Cells Bone Differentiation by Activating YAP/TAZ Pathway

Background: Hyaluronans exist in different forms, accordingly with molecular weight and degree of crosslinking. Here, we tested the capability to induce osteogenic differentiation in hDPSCs (human dental pulp stem cells) of three hyaluronans forms: linear pharmaceutical-grade hyaluronans at high and...

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Autores principales:	Marcella La Noce, Antonietta Stellavato, Valentina Vassallo, Marcella Cammarota, Luigi Laino, Vincenzo Desiderio, Vitale Del Vecchio, Giovanni Francesco Nicoletti, Virginia Tirino, Gianpaolo Papaccio, Chiara Schiraldi, Giuseppe Andrea Ferraro
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	hyaluronic acid dental pulp stem cells osteogenic differentiation YAP/TAZ pathway Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/05ac15be70454c1d978d35f00df44407
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spelling	oai:doaj.org-article:05ac15be70454c1d978d35f00df444072021-11-25T17:08:41ZHyaluronan-Based Gel Promotes Human Dental Pulp Stem Cells Bone Differentiation by Activating YAP/TAZ Pathway10.3390/cells101128992073-4409https://doaj.org/article/05ac15be70454c1d978d35f00df444072021-10-01T00:00:00Zhttps://www.mdpi.com/2073-4409/10/11/2899https://doaj.org/toc/2073-4409Background: Hyaluronans exist in different forms, accordingly with molecular weight and degree of crosslinking. Here, we tested the capability to induce osteogenic differentiation in hDPSCs (human dental pulp stem cells) of three hyaluronans forms: linear pharmaceutical-grade hyaluronans at high and (HHA) low molecular weight (LHA) and hybrid cooperative complexes (HCC), containing both sizes. Methods: hDPSCs were treated with HHA, LHA, HCC for 7, 14 and 21 days. The effects of hyaluronans on osteogenic differentiation were evaluated by qRT-PCR and WB of osteogenic markers and by Alizarin Red S staining. To identify the involved pathway, CD44 was analyzed by immunofluorescence, and YAP/TAZ expression was measured by qRT-PCR. Moreover, YAP/TAZ inhibitor-1 was used, and the loss of function of YAP/TAZ was evaluated by qRT-PCR, WB and immunofluorescence. Results: We showed that all hyaluronans improves osteogenesis. Among these, HCC is the main inducer of osteogenesis, along with overexpression of bone related markers and upregulating CD44. We also found that this biological process is subordinate to the activation of YAP/TAZ pathway. Conclusions: We found that HA’s molecular weight can have a relevant impact on HA performance for bone regeneration, and we unveil a new molecular mechanism by which HA acts on stem cells.Marcella La NoceAntonietta StellavatoValentina VassalloMarcella CammarotaLuigi LainoVincenzo DesiderioVitale Del VecchioGiovanni Francesco NicolettiVirginia TirinoGianpaolo PapaccioChiara SchiraldiGiuseppe Andrea FerraroMDPI AGarticlehyaluronic aciddental pulp stem cellsosteogenic differentiationYAP/TAZ pathwayBiology (General)QH301-705.5ENCells, Vol 10, Iss 2899, p 2899 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	hyaluronic acid dental pulp stem cells osteogenic differentiation YAP/TAZ pathway Biology (General) QH301-705.5
spellingShingle	hyaluronic acid dental pulp stem cells osteogenic differentiation YAP/TAZ pathway Biology (General) QH301-705.5 Marcella La Noce Antonietta Stellavato Valentina Vassallo Marcella Cammarota Luigi Laino Vincenzo Desiderio Vitale Del Vecchio Giovanni Francesco Nicoletti Virginia Tirino Gianpaolo Papaccio Chiara Schiraldi Giuseppe Andrea Ferraro Hyaluronan-Based Gel Promotes Human Dental Pulp Stem Cells Bone Differentiation by Activating YAP/TAZ Pathway
description	Background: Hyaluronans exist in different forms, accordingly with molecular weight and degree of crosslinking. Here, we tested the capability to induce osteogenic differentiation in hDPSCs (human dental pulp stem cells) of three hyaluronans forms: linear pharmaceutical-grade hyaluronans at high and (HHA) low molecular weight (LHA) and hybrid cooperative complexes (HCC), containing both sizes. Methods: hDPSCs were treated with HHA, LHA, HCC for 7, 14 and 21 days. The effects of hyaluronans on osteogenic differentiation were evaluated by qRT-PCR and WB of osteogenic markers and by Alizarin Red S staining. To identify the involved pathway, CD44 was analyzed by immunofluorescence, and YAP/TAZ expression was measured by qRT-PCR. Moreover, YAP/TAZ inhibitor-1 was used, and the loss of function of YAP/TAZ was evaluated by qRT-PCR, WB and immunofluorescence. Results: We showed that all hyaluronans improves osteogenesis. Among these, HCC is the main inducer of osteogenesis, along with overexpression of bone related markers and upregulating CD44. We also found that this biological process is subordinate to the activation of YAP/TAZ pathway. Conclusions: We found that HA’s molecular weight can have a relevant impact on HA performance for bone regeneration, and we unveil a new molecular mechanism by which HA acts on stem cells.
format	article
author	Marcella La Noce Antonietta Stellavato Valentina Vassallo Marcella Cammarota Luigi Laino Vincenzo Desiderio Vitale Del Vecchio Giovanni Francesco Nicoletti Virginia Tirino Gianpaolo Papaccio Chiara Schiraldi Giuseppe Andrea Ferraro
author_facet	Marcella La Noce Antonietta Stellavato Valentina Vassallo Marcella Cammarota Luigi Laino Vincenzo Desiderio Vitale Del Vecchio Giovanni Francesco Nicoletti Virginia Tirino Gianpaolo Papaccio Chiara Schiraldi Giuseppe Andrea Ferraro
author_sort	Marcella La Noce
title	Hyaluronan-Based Gel Promotes Human Dental Pulp Stem Cells Bone Differentiation by Activating YAP/TAZ Pathway
title_short	Hyaluronan-Based Gel Promotes Human Dental Pulp Stem Cells Bone Differentiation by Activating YAP/TAZ Pathway
title_full	Hyaluronan-Based Gel Promotes Human Dental Pulp Stem Cells Bone Differentiation by Activating YAP/TAZ Pathway
title_fullStr	Hyaluronan-Based Gel Promotes Human Dental Pulp Stem Cells Bone Differentiation by Activating YAP/TAZ Pathway
title_full_unstemmed	Hyaluronan-Based Gel Promotes Human Dental Pulp Stem Cells Bone Differentiation by Activating YAP/TAZ Pathway
title_sort	hyaluronan-based gel promotes human dental pulp stem cells bone differentiation by activating yap/taz pathway
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/05ac15be70454c1d978d35f00df44407
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Hyaluronan-Based Gel Promotes Human Dental Pulp Stem Cells Bone Differentiation by Activating YAP/TAZ Pathway

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