Vitamin D Modulation of TRAIL Expression in Human Milk and Mammary Epithelial Cells

Abstract The vitamin D levels in mothers affect the health status of both the mother and breastfeeding infant. Vitamin D deficient mothers’ infants are prone to rickets. While tumor necrosis factor-related apoptosis inducing ligand (TRAIL) has been implicated in cellular growth/apoptosis, immune cel...

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Autores principales:	Yuvaraj Sambandam, Sakamuri V. Reddy, Jennifer L. Mulligan, Christina Voelkel-Johnson, Carol L. Wagner
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/05be34985f264e98862e7a8b84356f84
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spelling	oai:doaj.org-article:05be34985f264e98862e7a8b84356f842021-12-02T16:06:39ZVitamin D Modulation of TRAIL Expression in Human Milk and Mammary Epithelial Cells10.1038/s41598-017-04521-y2045-2322https://doaj.org/article/05be34985f264e98862e7a8b84356f842017-06-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-04521-yhttps://doaj.org/toc/2045-2322Abstract The vitamin D levels in mothers affect the health status of both the mother and breastfeeding infant. Vitamin D deficient mothers’ infants are prone to rickets. While tumor necrosis factor-related apoptosis inducing ligand (TRAIL) has been implicated in cellular growth/apoptosis, immune cell function and bone-resorbing osteoclast formation, the expression of TRAIL in human milk as a function of vitamin D status in mothers remains unknown. We hypothesized that vitamin D deficiency alters TRAIL protein levels in human breast milk and mammary epithelial cells. Milk from vitamin D deficient mothers showed high levels of TRAIL (α and β) proteins compared to milk from vitamin D replete women. Western blot analysis of total cell lysate obtained from normal human mammary epithelial (HME-1) cells treated with variable doses (0–20 nM) of vitamin D for 24 h demonstrated that low levels (0.5 to 5 nM) significantly increased the TRAIL α but no change in β expression. In contrast, vitamin D at 20 nM concentration suppressed the expression of both TRAIL α and β proteins. Consistently, vitamin D regulated TRAIL mRNA expression in HME-1 cells. Our results indicate that vitamin D status in mothers modulates TRAIL expression in breast milk, which may have implications for both mother and infant health.Yuvaraj SambandamSakamuri V. ReddyJennifer L. MulliganChristina Voelkel-JohnsonCarol L. WagnerNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-7 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Yuvaraj Sambandam Sakamuri V. Reddy Jennifer L. Mulligan Christina Voelkel-Johnson Carol L. Wagner Vitamin D Modulation of TRAIL Expression in Human Milk and Mammary Epithelial Cells
description	Abstract The vitamin D levels in mothers affect the health status of both the mother and breastfeeding infant. Vitamin D deficient mothers’ infants are prone to rickets. While tumor necrosis factor-related apoptosis inducing ligand (TRAIL) has been implicated in cellular growth/apoptosis, immune cell function and bone-resorbing osteoclast formation, the expression of TRAIL in human milk as a function of vitamin D status in mothers remains unknown. We hypothesized that vitamin D deficiency alters TRAIL protein levels in human breast milk and mammary epithelial cells. Milk from vitamin D deficient mothers showed high levels of TRAIL (α and β) proteins compared to milk from vitamin D replete women. Western blot analysis of total cell lysate obtained from normal human mammary epithelial (HME-1) cells treated with variable doses (0–20 nM) of vitamin D for 24 h demonstrated that low levels (0.5 to 5 nM) significantly increased the TRAIL α but no change in β expression. In contrast, vitamin D at 20 nM concentration suppressed the expression of both TRAIL α and β proteins. Consistently, vitamin D regulated TRAIL mRNA expression in HME-1 cells. Our results indicate that vitamin D status in mothers modulates TRAIL expression in breast milk, which may have implications for both mother and infant health.
format	article
author	Yuvaraj Sambandam Sakamuri V. Reddy Jennifer L. Mulligan Christina Voelkel-Johnson Carol L. Wagner
author_facet	Yuvaraj Sambandam Sakamuri V. Reddy Jennifer L. Mulligan Christina Voelkel-Johnson Carol L. Wagner
author_sort	Yuvaraj Sambandam
title	Vitamin D Modulation of TRAIL Expression in Human Milk and Mammary Epithelial Cells
title_short	Vitamin D Modulation of TRAIL Expression in Human Milk and Mammary Epithelial Cells
title_full	Vitamin D Modulation of TRAIL Expression in Human Milk and Mammary Epithelial Cells
title_fullStr	Vitamin D Modulation of TRAIL Expression in Human Milk and Mammary Epithelial Cells
title_full_unstemmed	Vitamin D Modulation of TRAIL Expression in Human Milk and Mammary Epithelial Cells
title_sort	vitamin d modulation of trail expression in human milk and mammary epithelial cells
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/05be34985f264e98862e7a8b84356f84
work_keys_str_mv	AT yuvarajsambandam vitamindmodulationoftrailexpressioninhumanmilkandmammaryepithelialcells AT sakamurivreddy vitamindmodulationoftrailexpressioninhumanmilkandmammaryepithelialcells AT jenniferlmulligan vitamindmodulationoftrailexpressioninhumanmilkandmammaryepithelialcells AT christinavoelkeljohnson vitamindmodulationoftrailexpressioninhumanmilkandmammaryepithelialcells AT carollwagner vitamindmodulationoftrailexpressioninhumanmilkandmammaryepithelialcells
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Vitamin D Modulation of TRAIL Expression in Human Milk and Mammary Epithelial Cells

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