Characteristics of Carbapenem-Resistant Gram-Negative Bacilli in Patients with Ventilator-Associated Pneumonia

Carbapenem-resistant Gram-negative bacilli (CR-GNB) has become a global threat. In hospital settings, the association of CR-GNB with ventilator-associated pneumonia (VAP) is a critical public health concern owing to their high resistance rate to most antibiotics. The present study aims to identify t...

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Autores principales: Amira Mohamed, Enas Daef, Amany Nafie, Lamia Shaban, Maggie Ibrahim
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Publicado: MDPI AG 2021
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spelling oai:doaj.org-article:05c0c0e52c4c4dd3b65512a22acfca132021-11-25T16:22:52ZCharacteristics of Carbapenem-Resistant Gram-Negative Bacilli in Patients with Ventilator-Associated Pneumonia10.3390/antibiotics101113252079-6382https://doaj.org/article/05c0c0e52c4c4dd3b65512a22acfca132021-10-01T00:00:00Zhttps://www.mdpi.com/2079-6382/10/11/1325https://doaj.org/toc/2079-6382Carbapenem-resistant Gram-negative bacilli (CR-GNB) has become a global threat. In hospital settings, the association of CR-GNB with ventilator-associated pneumonia (VAP) is a critical public health concern owing to their high resistance rate to most antibiotics. The present study aims to identify the frequency of carbapenem-resistance and to determine the rate of multidrug resistance (MDR), extensive drug resistance (XDR) and pan-drug resistance (PDR) among CR-GNB infections in VAP. Antimicrobial susceptibility testing was carried out using the disk diffusion method and the detection of carbapenemases was screened using the imipenem-E test and the modified carbapenem-inactivation method (mCIM). The isolates were verified by polymerase chain reaction (PCR) for the presence of <i>bla</i><sub>NDM</sub>, <i>bla</i><sub>SPM</sub>, <i>bla</i><sub>VIM</sub>, <i>bla</i><sub>IMP</sub> and <i>bla</i><sub>GIM</sub> genes. 89.5%, 14%, 17.5%, 10.5%, 3.5% of isolates exhibited the presence of <i>bla</i><sub>NDM</sub>, <i>bla</i><sub>VIM</sub>, <i>bla</i><sub>SPM</sub>, <i>bla</i><sub>IMP</sub> and <i>bla</i><sub>GIM</sub>, respectively. 76%, 17% and 7% of isolates were PDR, XDR, and MDR, respectively. Carbapenem-resistance genes were identified in a significant percentage and <i>bla</i><sub>NDM</sub> was the most predominant gene. All isolates were highly resistant to most antibiotics. This health concern has proven to be a big challenge in developing countries such as Egypt, as it is associated with high morbidity, high mortality, and raised healthcare costs.Amira MohamedEnas DaefAmany NafieLamia ShabanMaggie IbrahimMDPI AGarticlecarbapenem-resistanceventilator-associated pneumoniaTherapeutics. PharmacologyRM1-950ENAntibiotics, Vol 10, Iss 1325, p 1325 (2021)
institution DOAJ
collection DOAJ
language EN
topic carbapenem-resistance
ventilator-associated pneumonia
Therapeutics. Pharmacology
RM1-950
spellingShingle carbapenem-resistance
ventilator-associated pneumonia
Therapeutics. Pharmacology
RM1-950
Amira Mohamed
Enas Daef
Amany Nafie
Lamia Shaban
Maggie Ibrahim
Characteristics of Carbapenem-Resistant Gram-Negative Bacilli in Patients with Ventilator-Associated Pneumonia
description Carbapenem-resistant Gram-negative bacilli (CR-GNB) has become a global threat. In hospital settings, the association of CR-GNB with ventilator-associated pneumonia (VAP) is a critical public health concern owing to their high resistance rate to most antibiotics. The present study aims to identify the frequency of carbapenem-resistance and to determine the rate of multidrug resistance (MDR), extensive drug resistance (XDR) and pan-drug resistance (PDR) among CR-GNB infections in VAP. Antimicrobial susceptibility testing was carried out using the disk diffusion method and the detection of carbapenemases was screened using the imipenem-E test and the modified carbapenem-inactivation method (mCIM). The isolates were verified by polymerase chain reaction (PCR) for the presence of <i>bla</i><sub>NDM</sub>, <i>bla</i><sub>SPM</sub>, <i>bla</i><sub>VIM</sub>, <i>bla</i><sub>IMP</sub> and <i>bla</i><sub>GIM</sub> genes. 89.5%, 14%, 17.5%, 10.5%, 3.5% of isolates exhibited the presence of <i>bla</i><sub>NDM</sub>, <i>bla</i><sub>VIM</sub>, <i>bla</i><sub>SPM</sub>, <i>bla</i><sub>IMP</sub> and <i>bla</i><sub>GIM</sub>, respectively. 76%, 17% and 7% of isolates were PDR, XDR, and MDR, respectively. Carbapenem-resistance genes were identified in a significant percentage and <i>bla</i><sub>NDM</sub> was the most predominant gene. All isolates were highly resistant to most antibiotics. This health concern has proven to be a big challenge in developing countries such as Egypt, as it is associated with high morbidity, high mortality, and raised healthcare costs.
format article
author Amira Mohamed
Enas Daef
Amany Nafie
Lamia Shaban
Maggie Ibrahim
author_facet Amira Mohamed
Enas Daef
Amany Nafie
Lamia Shaban
Maggie Ibrahim
author_sort Amira Mohamed
title Characteristics of Carbapenem-Resistant Gram-Negative Bacilli in Patients with Ventilator-Associated Pneumonia
title_short Characteristics of Carbapenem-Resistant Gram-Negative Bacilli in Patients with Ventilator-Associated Pneumonia
title_full Characteristics of Carbapenem-Resistant Gram-Negative Bacilli in Patients with Ventilator-Associated Pneumonia
title_fullStr Characteristics of Carbapenem-Resistant Gram-Negative Bacilli in Patients with Ventilator-Associated Pneumonia
title_full_unstemmed Characteristics of Carbapenem-Resistant Gram-Negative Bacilli in Patients with Ventilator-Associated Pneumonia
title_sort characteristics of carbapenem-resistant gram-negative bacilli in patients with ventilator-associated pneumonia
publisher MDPI AG
publishDate 2021
url https://doaj.org/article/05c0c0e52c4c4dd3b65512a22acfca13
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AT enasdaef characteristicsofcarbapenemresistantgramnegativebacilliinpatientswithventilatorassociatedpneumonia
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