Characteristics of Carbapenem-Resistant Gram-Negative Bacilli in Patients with Ventilator-Associated Pneumonia
Carbapenem-resistant Gram-negative bacilli (CR-GNB) has become a global threat. In hospital settings, the association of CR-GNB with ventilator-associated pneumonia (VAP) is a critical public health concern owing to their high resistance rate to most antibiotics. The present study aims to identify t...
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oai:doaj.org-article:05c0c0e52c4c4dd3b65512a22acfca132021-11-25T16:22:52ZCharacteristics of Carbapenem-Resistant Gram-Negative Bacilli in Patients with Ventilator-Associated Pneumonia10.3390/antibiotics101113252079-6382https://doaj.org/article/05c0c0e52c4c4dd3b65512a22acfca132021-10-01T00:00:00Zhttps://www.mdpi.com/2079-6382/10/11/1325https://doaj.org/toc/2079-6382Carbapenem-resistant Gram-negative bacilli (CR-GNB) has become a global threat. In hospital settings, the association of CR-GNB with ventilator-associated pneumonia (VAP) is a critical public health concern owing to their high resistance rate to most antibiotics. The present study aims to identify the frequency of carbapenem-resistance and to determine the rate of multidrug resistance (MDR), extensive drug resistance (XDR) and pan-drug resistance (PDR) among CR-GNB infections in VAP. Antimicrobial susceptibility testing was carried out using the disk diffusion method and the detection of carbapenemases was screened using the imipenem-E test and the modified carbapenem-inactivation method (mCIM). The isolates were verified by polymerase chain reaction (PCR) for the presence of <i>bla</i><sub>NDM</sub>, <i>bla</i><sub>SPM</sub>, <i>bla</i><sub>VIM</sub>, <i>bla</i><sub>IMP</sub> and <i>bla</i><sub>GIM</sub> genes. 89.5%, 14%, 17.5%, 10.5%, 3.5% of isolates exhibited the presence of <i>bla</i><sub>NDM</sub>, <i>bla</i><sub>VIM</sub>, <i>bla</i><sub>SPM</sub>, <i>bla</i><sub>IMP</sub> and <i>bla</i><sub>GIM</sub>, respectively. 76%, 17% and 7% of isolates were PDR, XDR, and MDR, respectively. Carbapenem-resistance genes were identified in a significant percentage and <i>bla</i><sub>NDM</sub> was the most predominant gene. All isolates were highly resistant to most antibiotics. This health concern has proven to be a big challenge in developing countries such as Egypt, as it is associated with high morbidity, high mortality, and raised healthcare costs.Amira MohamedEnas DaefAmany NafieLamia ShabanMaggie IbrahimMDPI AGarticlecarbapenem-resistanceventilator-associated pneumoniaTherapeutics. PharmacologyRM1-950ENAntibiotics, Vol 10, Iss 1325, p 1325 (2021) |
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carbapenem-resistance ventilator-associated pneumonia Therapeutics. Pharmacology RM1-950 |
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carbapenem-resistance ventilator-associated pneumonia Therapeutics. Pharmacology RM1-950 Amira Mohamed Enas Daef Amany Nafie Lamia Shaban Maggie Ibrahim Characteristics of Carbapenem-Resistant Gram-Negative Bacilli in Patients with Ventilator-Associated Pneumonia |
description |
Carbapenem-resistant Gram-negative bacilli (CR-GNB) has become a global threat. In hospital settings, the association of CR-GNB with ventilator-associated pneumonia (VAP) is a critical public health concern owing to their high resistance rate to most antibiotics. The present study aims to identify the frequency of carbapenem-resistance and to determine the rate of multidrug resistance (MDR), extensive drug resistance (XDR) and pan-drug resistance (PDR) among CR-GNB infections in VAP. Antimicrobial susceptibility testing was carried out using the disk diffusion method and the detection of carbapenemases was screened using the imipenem-E test and the modified carbapenem-inactivation method (mCIM). The isolates were verified by polymerase chain reaction (PCR) for the presence of <i>bla</i><sub>NDM</sub>, <i>bla</i><sub>SPM</sub>, <i>bla</i><sub>VIM</sub>, <i>bla</i><sub>IMP</sub> and <i>bla</i><sub>GIM</sub> genes. 89.5%, 14%, 17.5%, 10.5%, 3.5% of isolates exhibited the presence of <i>bla</i><sub>NDM</sub>, <i>bla</i><sub>VIM</sub>, <i>bla</i><sub>SPM</sub>, <i>bla</i><sub>IMP</sub> and <i>bla</i><sub>GIM</sub>, respectively. 76%, 17% and 7% of isolates were PDR, XDR, and MDR, respectively. Carbapenem-resistance genes were identified in a significant percentage and <i>bla</i><sub>NDM</sub> was the most predominant gene. All isolates were highly resistant to most antibiotics. This health concern has proven to be a big challenge in developing countries such as Egypt, as it is associated with high morbidity, high mortality, and raised healthcare costs. |
format |
article |
author |
Amira Mohamed Enas Daef Amany Nafie Lamia Shaban Maggie Ibrahim |
author_facet |
Amira Mohamed Enas Daef Amany Nafie Lamia Shaban Maggie Ibrahim |
author_sort |
Amira Mohamed |
title |
Characteristics of Carbapenem-Resistant Gram-Negative Bacilli in Patients with Ventilator-Associated Pneumonia |
title_short |
Characteristics of Carbapenem-Resistant Gram-Negative Bacilli in Patients with Ventilator-Associated Pneumonia |
title_full |
Characteristics of Carbapenem-Resistant Gram-Negative Bacilli in Patients with Ventilator-Associated Pneumonia |
title_fullStr |
Characteristics of Carbapenem-Resistant Gram-Negative Bacilli in Patients with Ventilator-Associated Pneumonia |
title_full_unstemmed |
Characteristics of Carbapenem-Resistant Gram-Negative Bacilli in Patients with Ventilator-Associated Pneumonia |
title_sort |
characteristics of carbapenem-resistant gram-negative bacilli in patients with ventilator-associated pneumonia |
publisher |
MDPI AG |
publishDate |
2021 |
url |
https://doaj.org/article/05c0c0e52c4c4dd3b65512a22acfca13 |
work_keys_str_mv |
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_version_ |
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