Characteristics of Carbapenem-Resistant Gram-Negative Bacilli in Patients with Ventilator-Associated Pneumonia

Carbapenem-resistant Gram-negative bacilli (CR-GNB) has become a global threat. In hospital settings, the association of CR-GNB with ventilator-associated pneumonia (VAP) is a critical public health concern owing to their high resistance rate to most antibiotics. The present study aims to identify t...

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Auteurs principaux:	Amira Mohamed, Enas Daef, Amany Nafie, Lamia Shaban, Maggie Ibrahim
Format:	article
Langue:	EN
Publié:	MDPI AG 2021
Sujets:	carbapenem-resistance ventilator-associated pneumonia Therapeutics. Pharmacology RM1-950
Accès en ligne:	https://doaj.org/article/05c0c0e52c4c4dd3b65512a22acfca13
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spelling	oai:doaj.org-article:05c0c0e52c4c4dd3b65512a22acfca132021-11-25T16:22:52ZCharacteristics of Carbapenem-Resistant Gram-Negative Bacilli in Patients with Ventilator-Associated Pneumonia10.3390/antibiotics101113252079-6382https://doaj.org/article/05c0c0e52c4c4dd3b65512a22acfca132021-10-01T00:00:00Zhttps://www.mdpi.com/2079-6382/10/11/1325https://doaj.org/toc/2079-6382Carbapenem-resistant Gram-negative bacilli (CR-GNB) has become a global threat. In hospital settings, the association of CR-GNB with ventilator-associated pneumonia (VAP) is a critical public health concern owing to their high resistance rate to most antibiotics. The present study aims to identify the frequency of carbapenem-resistance and to determine the rate of multidrug resistance (MDR), extensive drug resistance (XDR) and pan-drug resistance (PDR) among CR-GNB infections in VAP. Antimicrobial susceptibility testing was carried out using the disk diffusion method and the detection of carbapenemases was screened using the imipenem-E test and the modified carbapenem-inactivation method (mCIM). The isolates were verified by polymerase chain reaction (PCR) for the presence of <i>bla</i><sub>NDM</sub>, <i>bla</i><sub>SPM</sub>, <i>bla</i><sub>VIM</sub>, <i>bla</i><sub>IMP</sub> and <i>bla</i><sub>GIM</sub> genes. 89.5%, 14%, 17.5%, 10.5%, 3.5% of isolates exhibited the presence of <i>bla</i><sub>NDM</sub>, <i>bla</i><sub>VIM</sub>, <i>bla</i><sub>SPM</sub>, <i>bla</i><sub>IMP</sub> and <i>bla</i><sub>GIM</sub>, respectively. 76%, 17% and 7% of isolates were PDR, XDR, and MDR, respectively. Carbapenem-resistance genes were identified in a significant percentage and <i>bla</i><sub>NDM</sub> was the most predominant gene. All isolates were highly resistant to most antibiotics. This health concern has proven to be a big challenge in developing countries such as Egypt, as it is associated with high morbidity, high mortality, and raised healthcare costs.Amira MohamedEnas DaefAmany NafieLamia ShabanMaggie IbrahimMDPI AGarticlecarbapenem-resistanceventilator-associated pneumoniaTherapeutics. PharmacologyRM1-950ENAntibiotics, Vol 10, Iss 1325, p 1325 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	carbapenem-resistance ventilator-associated pneumonia Therapeutics. Pharmacology RM1-950
spellingShingle	carbapenem-resistance ventilator-associated pneumonia Therapeutics. Pharmacology RM1-950 Amira Mohamed Enas Daef Amany Nafie Lamia Shaban Maggie Ibrahim Characteristics of Carbapenem-Resistant Gram-Negative Bacilli in Patients with Ventilator-Associated Pneumonia
description	Carbapenem-resistant Gram-negative bacilli (CR-GNB) has become a global threat. In hospital settings, the association of CR-GNB with ventilator-associated pneumonia (VAP) is a critical public health concern owing to their high resistance rate to most antibiotics. The present study aims to identify the frequency of carbapenem-resistance and to determine the rate of multidrug resistance (MDR), extensive drug resistance (XDR) and pan-drug resistance (PDR) among CR-GNB infections in VAP. Antimicrobial susceptibility testing was carried out using the disk diffusion method and the detection of carbapenemases was screened using the imipenem-E test and the modified carbapenem-inactivation method (mCIM). The isolates were verified by polymerase chain reaction (PCR) for the presence of <i>bla</i><sub>NDM</sub>, <i>bla</i><sub>SPM</sub>, <i>bla</i><sub>VIM</sub>, <i>bla</i><sub>IMP</sub> and <i>bla</i><sub>GIM</sub> genes. 89.5%, 14%, 17.5%, 10.5%, 3.5% of isolates exhibited the presence of <i>bla</i><sub>NDM</sub>, <i>bla</i><sub>VIM</sub>, <i>bla</i><sub>SPM</sub>, <i>bla</i><sub>IMP</sub> and <i>bla</i><sub>GIM</sub>, respectively. 76%, 17% and 7% of isolates were PDR, XDR, and MDR, respectively. Carbapenem-resistance genes were identified in a significant percentage and <i>bla</i><sub>NDM</sub> was the most predominant gene. All isolates were highly resistant to most antibiotics. This health concern has proven to be a big challenge in developing countries such as Egypt, as it is associated with high morbidity, high mortality, and raised healthcare costs.
format	article
author	Amira Mohamed Enas Daef Amany Nafie Lamia Shaban Maggie Ibrahim
author_facet	Amira Mohamed Enas Daef Amany Nafie Lamia Shaban Maggie Ibrahim
author_sort	Amira Mohamed
title	Characteristics of Carbapenem-Resistant Gram-Negative Bacilli in Patients with Ventilator-Associated Pneumonia
title_short	Characteristics of Carbapenem-Resistant Gram-Negative Bacilli in Patients with Ventilator-Associated Pneumonia
title_full	Characteristics of Carbapenem-Resistant Gram-Negative Bacilli in Patients with Ventilator-Associated Pneumonia
title_fullStr	Characteristics of Carbapenem-Resistant Gram-Negative Bacilli in Patients with Ventilator-Associated Pneumonia
title_full_unstemmed	Characteristics of Carbapenem-Resistant Gram-Negative Bacilli in Patients with Ventilator-Associated Pneumonia
title_sort	characteristics of carbapenem-resistant gram-negative bacilli in patients with ventilator-associated pneumonia
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/05c0c0e52c4c4dd3b65512a22acfca13
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Characteristics of Carbapenem-Resistant Gram-Negative Bacilli in Patients with Ventilator-Associated Pneumonia

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