Inclusion of double helix structural oligonucleotide (STexS) results in an enhance of SNP specificity in PCR

Abstract Genetic mutations such as single nucleotide polymorphisms (SNP) are known as one of the most common forms which related to various genetic disorders and cancers. Among of the methods developed for efficient detection of such SNP, polymerase chain reaction (PCR) methods are widely used world...

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Autores principales:	Jae Jong Kim, Hyoung-Min Park, A. Young Kyoung, In Kyung Park, Si-Kyu Lim, Byoung Chul Park
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/05c4f1c7ff894b5abf7b1dd2a0a5641b
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spelling	oai:doaj.org-article:05c4f1c7ff894b5abf7b1dd2a0a5641b2021-12-02T19:16:47ZInclusion of double helix structural oligonucleotide (STexS) results in an enhance of SNP specificity in PCR10.1038/s41598-021-98610-82045-2322https://doaj.org/article/05c4f1c7ff894b5abf7b1dd2a0a5641b2021-09-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-98610-8https://doaj.org/toc/2045-2322Abstract Genetic mutations such as single nucleotide polymorphisms (SNP) are known as one of the most common forms which related to various genetic disorders and cancers. Among of the methods developed for efficient detection of such SNP, polymerase chain reaction (PCR) methods are widely used worldwide for its cost and viable advantages. However, the technique to discriminate small amounts of SNP mixed in abundant normal DNA is incomplete due to intrinsic technical problems of PCR such as amplification occurring even in 3’mismatched cases because of high enzyme activity of DNA polymerases. To overcome the issue, specifically designed PCR platform, STexS (SNP typing with excellent specificity) using double stranded oligonucleotides was implemented as a means to emphasize the amplification of SNP templates by decreasing unwanted amplification of 3’mismatched DNA copies. In this study, the results indicate several EGFR mutations were easily detected specifically utilizing the STexS platform. Further trials show the novel method works effectively to discriminate mutations in not only general allele specific (AS)-PCRs, but also amplification refractory mutation system (ARMS)-PCR. The STexS platform will give aid in PCRs targeting potential SNPs or genetically mutated biomarkers in human clinical samples.Jae Jong KimHyoung-Min ParkA. Young KyoungIn Kyung ParkSi-Kyu LimByoung Chul ParkNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-7 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Jae Jong Kim Hyoung-Min Park A. Young Kyoung In Kyung Park Si-Kyu Lim Byoung Chul Park Inclusion of double helix structural oligonucleotide (STexS) results in an enhance of SNP specificity in PCR
description	Abstract Genetic mutations such as single nucleotide polymorphisms (SNP) are known as one of the most common forms which related to various genetic disorders and cancers. Among of the methods developed for efficient detection of such SNP, polymerase chain reaction (PCR) methods are widely used worldwide for its cost and viable advantages. However, the technique to discriminate small amounts of SNP mixed in abundant normal DNA is incomplete due to intrinsic technical problems of PCR such as amplification occurring even in 3’mismatched cases because of high enzyme activity of DNA polymerases. To overcome the issue, specifically designed PCR platform, STexS (SNP typing with excellent specificity) using double stranded oligonucleotides was implemented as a means to emphasize the amplification of SNP templates by decreasing unwanted amplification of 3’mismatched DNA copies. In this study, the results indicate several EGFR mutations were easily detected specifically utilizing the STexS platform. Further trials show the novel method works effectively to discriminate mutations in not only general allele specific (AS)-PCRs, but also amplification refractory mutation system (ARMS)-PCR. The STexS platform will give aid in PCRs targeting potential SNPs or genetically mutated biomarkers in human clinical samples.
format	article
author	Jae Jong Kim Hyoung-Min Park A. Young Kyoung In Kyung Park Si-Kyu Lim Byoung Chul Park
author_facet	Jae Jong Kim Hyoung-Min Park A. Young Kyoung In Kyung Park Si-Kyu Lim Byoung Chul Park
author_sort	Jae Jong Kim
title	Inclusion of double helix structural oligonucleotide (STexS) results in an enhance of SNP specificity in PCR
title_short	Inclusion of double helix structural oligonucleotide (STexS) results in an enhance of SNP specificity in PCR
title_full	Inclusion of double helix structural oligonucleotide (STexS) results in an enhance of SNP specificity in PCR
title_fullStr	Inclusion of double helix structural oligonucleotide (STexS) results in an enhance of SNP specificity in PCR
title_full_unstemmed	Inclusion of double helix structural oligonucleotide (STexS) results in an enhance of SNP specificity in PCR
title_sort	inclusion of double helix structural oligonucleotide (stexs) results in an enhance of snp specificity in pcr
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/05c4f1c7ff894b5abf7b1dd2a0a5641b
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Inclusion of double helix structural oligonucleotide (STexS) results in an enhance of SNP specificity in PCR

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