Dominance effects of deleterious and beneficial mutations in a single gene of the RNA virus ϕ6.

Most of our knowledge of dominance stems from studies of deleterious mutations. From these studies we know that most deleterious mutations are recessive, and that this recessivity arises from a hyperbolic relationship between protein function (i.e., protein concentration or activity) and fitness. He...

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Autores principales:	Sarah B Joseph, Kayla M Peck, Christina L Burch
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2014
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/05cc9c76380c4372baf4149e07af97b3
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spelling	oai:doaj.org-article:05cc9c76380c4372baf4149e07af97b32021-11-18T08:14:57ZDominance effects of deleterious and beneficial mutations in a single gene of the RNA virus ϕ6.1932-620310.1371/journal.pone.0097717https://doaj.org/article/05cc9c76380c4372baf4149e07af97b32014-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/24945910/?tool=EBIhttps://doaj.org/toc/1932-6203Most of our knowledge of dominance stems from studies of deleterious mutations. From these studies we know that most deleterious mutations are recessive, and that this recessivity arises from a hyperbolic relationship between protein function (i.e., protein concentration or activity) and fitness. Here we investigate whether this knowledge can be used to make predictions about the dominance of beneficial and deleterious mutations in a single gene. We employed a model system--the bacteriophage φ6--that allowed us to generate a collection of mutations in haploid conditions so that it was not biased toward either dominant beneficial or recessive deleterious mutations. Screening for the ability to infect a bacterial host that does not permit infection by the wildtype φ6, we generated a collection of mutations in P3, a gene involved in attachment to the host and in phage particle assembly. The resulting collection contained mutations with both deleterious and beneficial effects on fitness. The deleterious mutations in our collection had additive effects on fitness and the beneficial mutations were recessive. Neither of these observations were predicted from previous studies of dominance. This pattern is not consistent with the hyperbolic (diminishing returns) relationship between protein function and fitness that is characteristic of enzymatic genes, but could have resulted from a curve of increasing returns.Sarah B JosephKayla M PeckChristina L BurchPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 9, Iss 6, p e97717 (2014)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Sarah B Joseph Kayla M Peck Christina L Burch Dominance effects of deleterious and beneficial mutations in a single gene of the RNA virus ϕ6.
description	Most of our knowledge of dominance stems from studies of deleterious mutations. From these studies we know that most deleterious mutations are recessive, and that this recessivity arises from a hyperbolic relationship between protein function (i.e., protein concentration or activity) and fitness. Here we investigate whether this knowledge can be used to make predictions about the dominance of beneficial and deleterious mutations in a single gene. We employed a model system--the bacteriophage φ6--that allowed us to generate a collection of mutations in haploid conditions so that it was not biased toward either dominant beneficial or recessive deleterious mutations. Screening for the ability to infect a bacterial host that does not permit infection by the wildtype φ6, we generated a collection of mutations in P3, a gene involved in attachment to the host and in phage particle assembly. The resulting collection contained mutations with both deleterious and beneficial effects on fitness. The deleterious mutations in our collection had additive effects on fitness and the beneficial mutations were recessive. Neither of these observations were predicted from previous studies of dominance. This pattern is not consistent with the hyperbolic (diminishing returns) relationship between protein function and fitness that is characteristic of enzymatic genes, but could have resulted from a curve of increasing returns.
format	article
author	Sarah B Joseph Kayla M Peck Christina L Burch
author_facet	Sarah B Joseph Kayla M Peck Christina L Burch
author_sort	Sarah B Joseph
title	Dominance effects of deleterious and beneficial mutations in a single gene of the RNA virus ϕ6.
title_short	Dominance effects of deleterious and beneficial mutations in a single gene of the RNA virus ϕ6.
title_full	Dominance effects of deleterious and beneficial mutations in a single gene of the RNA virus ϕ6.
title_fullStr	Dominance effects of deleterious and beneficial mutations in a single gene of the RNA virus ϕ6.
title_full_unstemmed	Dominance effects of deleterious and beneficial mutations in a single gene of the RNA virus ϕ6.
title_sort	dominance effects of deleterious and beneficial mutations in a single gene of the rna virus ϕ6.
publisher	Public Library of Science (PLoS)
publishDate	2014
url	https://doaj.org/article/05cc9c76380c4372baf4149e07af97b3
work_keys_str_mv	AT sarahbjoseph dominanceeffectsofdeleteriousandbeneficialmutationsinasinglegeneofthernavirusph6 AT kaylampeck dominanceeffectsofdeleteriousandbeneficialmutationsinasinglegeneofthernavirusph6 AT christinalburch dominanceeffectsofdeleteriousandbeneficialmutationsinasinglegeneofthernavirusph6
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Dominance effects of deleterious and beneficial mutations in a single gene of the RNA virus ϕ6.

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