Transcriptomic Changes in Internode Explants of Stinging Nettle during Callogenesis

Callogenesis, the process during which explants derived from differentiated plant tissues are subjected to a trans-differentiation step characterized by the proliferation of a mass of cells, is fundamental to indirect organogenesis and the establishment of cell suspension cultures. Therefore, unders...

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Autores principales:	Xuan Xu, Sylvain Legay, Roberto Berni, Jean-Francois Hausman, Gea Guerriero
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	callogenesis transcriptomics stinging nettle qPCR plant growth regulators Biology (General) QH301-705.5 Chemistry QD1-999
Acceso en línea:	https://doaj.org/article/05d39f4c3b2b40ec846b41c78bcc4b59
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spelling	oai:doaj.org-article:05d39f4c3b2b40ec846b41c78bcc4b592021-11-25T17:55:23ZTranscriptomic Changes in Internode Explants of Stinging Nettle during Callogenesis10.3390/ijms2222123191422-00671661-6596https://doaj.org/article/05d39f4c3b2b40ec846b41c78bcc4b592021-11-01T00:00:00Zhttps://www.mdpi.com/1422-0067/22/22/12319https://doaj.org/toc/1661-6596https://doaj.org/toc/1422-0067Callogenesis, the process during which explants derived from differentiated plant tissues are subjected to a trans-differentiation step characterized by the proliferation of a mass of cells, is fundamental to indirect organogenesis and the establishment of cell suspension cultures. Therefore, understanding how callogenesis takes place is helpful to plant tissue culture, as well as to plant biotechnology and bioprocess engineering. The common herbaceous plant stinging nettle (<i>Urtica dioica</i> L.) is a species producing cellulosic fibres (the bast fibres) and a whole array of phytochemicals for pharmacological, nutraceutical and cosmeceutical use. Thus, it is of interest as a potential multi-purpose plant. In this study, callogenesis in internode explants of a nettle fibre clone (clone 13) was studied using RNA-Seq to understand which gene ontologies predominate at different time points. Callogenesis was induced with the plant growth regulators α-napthaleneacetic acid (NAA) and 6-benzyl aminopurine (BAP) after having determined their optimal concentrations. The process was studied over a period of 34 days, a time point at which a well-visible callus mass developed on the explants. The bioinformatic analysis of the transcriptomic dataset revealed specific gene ontologies characterizing each of the four time points investigated (0, 1, 10 and 34 days). The results show that, while the advanced stage of callogenesis is characterized by the iron deficiency response triggered by the high levels of reactive oxygen species accumulated by the proliferating cell mass, the intermediate and early phases are dominated by ontologies related to the immune response and cell wall loosening, respectively.Xuan XuSylvain LegayRoberto BerniJean-Francois HausmanGea GuerrieroMDPI AGarticlecallogenesistranscriptomicsstinging nettleqPCRplant growth regulatorsBiology (General)QH301-705.5ChemistryQD1-999ENInternational Journal of Molecular Sciences, Vol 22, Iss 12319, p 12319 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	callogenesis transcriptomics stinging nettle qPCR plant growth regulators Biology (General) QH301-705.5 Chemistry QD1-999
spellingShingle	callogenesis transcriptomics stinging nettle qPCR plant growth regulators Biology (General) QH301-705.5 Chemistry QD1-999 Xuan Xu Sylvain Legay Roberto Berni Jean-Francois Hausman Gea Guerriero Transcriptomic Changes in Internode Explants of Stinging Nettle during Callogenesis
description	Callogenesis, the process during which explants derived from differentiated plant tissues are subjected to a trans-differentiation step characterized by the proliferation of a mass of cells, is fundamental to indirect organogenesis and the establishment of cell suspension cultures. Therefore, understanding how callogenesis takes place is helpful to plant tissue culture, as well as to plant biotechnology and bioprocess engineering. The common herbaceous plant stinging nettle (<i>Urtica dioica</i> L.) is a species producing cellulosic fibres (the bast fibres) and a whole array of phytochemicals for pharmacological, nutraceutical and cosmeceutical use. Thus, it is of interest as a potential multi-purpose plant. In this study, callogenesis in internode explants of a nettle fibre clone (clone 13) was studied using RNA-Seq to understand which gene ontologies predominate at different time points. Callogenesis was induced with the plant growth regulators α-napthaleneacetic acid (NAA) and 6-benzyl aminopurine (BAP) after having determined their optimal concentrations. The process was studied over a period of 34 days, a time point at which a well-visible callus mass developed on the explants. The bioinformatic analysis of the transcriptomic dataset revealed specific gene ontologies characterizing each of the four time points investigated (0, 1, 10 and 34 days). The results show that, while the advanced stage of callogenesis is characterized by the iron deficiency response triggered by the high levels of reactive oxygen species accumulated by the proliferating cell mass, the intermediate and early phases are dominated by ontologies related to the immune response and cell wall loosening, respectively.
format	article
author	Xuan Xu Sylvain Legay Roberto Berni Jean-Francois Hausman Gea Guerriero
author_facet	Xuan Xu Sylvain Legay Roberto Berni Jean-Francois Hausman Gea Guerriero
author_sort	Xuan Xu
title	Transcriptomic Changes in Internode Explants of Stinging Nettle during Callogenesis
title_short	Transcriptomic Changes in Internode Explants of Stinging Nettle during Callogenesis
title_full	Transcriptomic Changes in Internode Explants of Stinging Nettle during Callogenesis
title_fullStr	Transcriptomic Changes in Internode Explants of Stinging Nettle during Callogenesis
title_full_unstemmed	Transcriptomic Changes in Internode Explants of Stinging Nettle during Callogenesis
title_sort	transcriptomic changes in internode explants of stinging nettle during callogenesis
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/05d39f4c3b2b40ec846b41c78bcc4b59
work_keys_str_mv	AT xuanxu transcriptomicchangesininternodeexplantsofstingingnettleduringcallogenesis AT sylvainlegay transcriptomicchangesininternodeexplantsofstingingnettleduringcallogenesis AT robertoberni transcriptomicchangesininternodeexplantsofstingingnettleduringcallogenesis AT jeanfrancoishausman transcriptomicchangesininternodeexplantsofstingingnettleduringcallogenesis AT geaguerriero transcriptomicchangesininternodeexplantsofstingingnettleduringcallogenesis
_version_	1718411842194243584

Transcriptomic Changes in Internode Explants of Stinging Nettle during Callogenesis

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