Stochastic model of contact inhibition and the proliferation of melanoma in situ

Abstract Contact inhibition is a central feature orchestrating cell proliferation in culture experiments; its loss is associated with malignant transformation and tumorigenesis. We performed a co-culture experiment with human metastatic melanoma cell line (SKMEL- 147) and immortalized keratinocyte c...

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Autores principales:	Mauro César Cafundó Morais, Izabella Stuhl, Alan U. Sabino, Willian W. Lautenschlager, Alexandre S. Queiroga, Tharcisio Citrangulo Tortelli, Roger Chammas, Yuri Suhov, Alexandre F. Ramos
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/05de95ad2ade4a07b8dde3cf5844f427
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spelling	oai:doaj.org-article:05de95ad2ade4a07b8dde3cf5844f4272021-12-02T12:30:52ZStochastic model of contact inhibition and the proliferation of melanoma in situ10.1038/s41598-017-07553-62045-2322https://doaj.org/article/05de95ad2ade4a07b8dde3cf5844f4272017-08-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-07553-6https://doaj.org/toc/2045-2322Abstract Contact inhibition is a central feature orchestrating cell proliferation in culture experiments; its loss is associated with malignant transformation and tumorigenesis. We performed a co-culture experiment with human metastatic melanoma cell line (SKMEL- 147) and immortalized keratinocyte cells (HaCaT). After 8 days a spatial pattern was detected, characterized by the formation of clusters of melanoma cells surrounded by keratinocytes constraining their proliferation. In addition, we observed that the proportion of melanoma cells within the total population has increased. To explain our results we propose a spatial stochastic model (following a philosophy of the Widom-Rowlinson model from Statistical Physics and Molecular Chemistry) which considers cell proliferation, death, migration, and cell-to-cell interaction through contact inhibition. Our numerical simulations demonstrate that loss of contact inhibition is a sufficient mechanism, appropriate for an explanation of the increase in the proportion of tumor cells and generation of spatial patterns established in the conducted experiments.Mauro César Cafundó MoraisIzabella StuhlAlan U. SabinoWillian W. LautenschlagerAlexandre S. QueirogaTharcisio Citrangulo TortelliRoger ChammasYuri SuhovAlexandre F. RamosNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-9 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Mauro César Cafundó Morais Izabella Stuhl Alan U. Sabino Willian W. Lautenschlager Alexandre S. Queiroga Tharcisio Citrangulo Tortelli Roger Chammas Yuri Suhov Alexandre F. Ramos Stochastic model of contact inhibition and the proliferation of melanoma in situ
description	Abstract Contact inhibition is a central feature orchestrating cell proliferation in culture experiments; its loss is associated with malignant transformation and tumorigenesis. We performed a co-culture experiment with human metastatic melanoma cell line (SKMEL- 147) and immortalized keratinocyte cells (HaCaT). After 8 days a spatial pattern was detected, characterized by the formation of clusters of melanoma cells surrounded by keratinocytes constraining their proliferation. In addition, we observed that the proportion of melanoma cells within the total population has increased. To explain our results we propose a spatial stochastic model (following a philosophy of the Widom-Rowlinson model from Statistical Physics and Molecular Chemistry) which considers cell proliferation, death, migration, and cell-to-cell interaction through contact inhibition. Our numerical simulations demonstrate that loss of contact inhibition is a sufficient mechanism, appropriate for an explanation of the increase in the proportion of tumor cells and generation of spatial patterns established in the conducted experiments.
format	article
author	Mauro César Cafundó Morais Izabella Stuhl Alan U. Sabino Willian W. Lautenschlager Alexandre S. Queiroga Tharcisio Citrangulo Tortelli Roger Chammas Yuri Suhov Alexandre F. Ramos
author_facet	Mauro César Cafundó Morais Izabella Stuhl Alan U. Sabino Willian W. Lautenschlager Alexandre S. Queiroga Tharcisio Citrangulo Tortelli Roger Chammas Yuri Suhov Alexandre F. Ramos
author_sort	Mauro César Cafundó Morais
title	Stochastic model of contact inhibition and the proliferation of melanoma in situ
title_short	Stochastic model of contact inhibition and the proliferation of melanoma in situ
title_full	Stochastic model of contact inhibition and the proliferation of melanoma in situ
title_fullStr	Stochastic model of contact inhibition and the proliferation of melanoma in situ
title_full_unstemmed	Stochastic model of contact inhibition and the proliferation of melanoma in situ
title_sort	stochastic model of contact inhibition and the proliferation of melanoma in situ
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/05de95ad2ade4a07b8dde3cf5844f427
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Stochastic model of contact inhibition and the proliferation of melanoma in situ

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