Membrane-elasticity model of Coatless vesicle budding induced by ESCRT complexes.

The formation of vesicles is essential for many biological processes, in particular for the trafficking of membrane proteins within cells. The Endosomal Sorting Complex Required for Transport (ESCRT) directs membrane budding away from the cytosol. Unlike other vesicle formation pathways, the ESCRT-m...

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Autores principales:	Bartosz Różycki, Evzen Boura, James H Hurley, Gerhard Hummer
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2012
Materias:	Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/05e2bb677dd04a869573878b655422b9
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spelling	oai:doaj.org-article:05e2bb677dd04a869573878b655422b92021-11-18T05:52:46ZMembrane-elasticity model of Coatless vesicle budding induced by ESCRT complexes.1553-734X1553-735810.1371/journal.pcbi.1002736https://doaj.org/article/05e2bb677dd04a869573878b655422b92012-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/23093927/pdf/?tool=EBIhttps://doaj.org/toc/1553-734Xhttps://doaj.org/toc/1553-7358The formation of vesicles is essential for many biological processes, in particular for the trafficking of membrane proteins within cells. The Endosomal Sorting Complex Required for Transport (ESCRT) directs membrane budding away from the cytosol. Unlike other vesicle formation pathways, the ESCRT-mediated budding occurs without a protein coat. Here, we propose a minimal model of ESCRT-induced vesicle budding. Our model is based on recent experimental observations from direct fluorescence microscopy imaging that show ESCRT proteins colocalized only in the neck region of membrane buds. The model, cast in the framework of membrane elasticity theory, reproduces the experimentally observed vesicle morphologies with physically meaningful parameters. In this parameter range, the minimum energy configurations of the membrane are coatless buds with ESCRTs localized in the bud neck, consistent with experiment. The minimum energy configurations agree with those seen in the fluorescence images, with respect to both bud shapes and ESCRT protein localization. On the basis of our model, we identify distinct mechanistic pathways for the ESCRT-mediated budding process. The bud size is determined by membrane material parameters, explaining the narrow yet different bud size distributions in vitro and in vivo. Our membrane elasticity model thus sheds light on the energetics and possible mechanisms of ESCRT-induced membrane budding.Bartosz RóżyckiEvzen BouraJames H HurleyGerhard HummerPublic Library of Science (PLoS)articleBiology (General)QH301-705.5ENPLoS Computational Biology, Vol 8, Iss 10, p e1002736 (2012)
institution	DOAJ
collection	DOAJ
language	EN
topic	Biology (General) QH301-705.5
spellingShingle	Biology (General) QH301-705.5 Bartosz Różycki Evzen Boura James H Hurley Gerhard Hummer Membrane-elasticity model of Coatless vesicle budding induced by ESCRT complexes.
description	The formation of vesicles is essential for many biological processes, in particular for the trafficking of membrane proteins within cells. The Endosomal Sorting Complex Required for Transport (ESCRT) directs membrane budding away from the cytosol. Unlike other vesicle formation pathways, the ESCRT-mediated budding occurs without a protein coat. Here, we propose a minimal model of ESCRT-induced vesicle budding. Our model is based on recent experimental observations from direct fluorescence microscopy imaging that show ESCRT proteins colocalized only in the neck region of membrane buds. The model, cast in the framework of membrane elasticity theory, reproduces the experimentally observed vesicle morphologies with physically meaningful parameters. In this parameter range, the minimum energy configurations of the membrane are coatless buds with ESCRTs localized in the bud neck, consistent with experiment. The minimum energy configurations agree with those seen in the fluorescence images, with respect to both bud shapes and ESCRT protein localization. On the basis of our model, we identify distinct mechanistic pathways for the ESCRT-mediated budding process. The bud size is determined by membrane material parameters, explaining the narrow yet different bud size distributions in vitro and in vivo. Our membrane elasticity model thus sheds light on the energetics and possible mechanisms of ESCRT-induced membrane budding.
format	article
author	Bartosz Różycki Evzen Boura James H Hurley Gerhard Hummer
author_facet	Bartosz Różycki Evzen Boura James H Hurley Gerhard Hummer
author_sort	Bartosz Różycki
title	Membrane-elasticity model of Coatless vesicle budding induced by ESCRT complexes.
title_short	Membrane-elasticity model of Coatless vesicle budding induced by ESCRT complexes.
title_full	Membrane-elasticity model of Coatless vesicle budding induced by ESCRT complexes.
title_fullStr	Membrane-elasticity model of Coatless vesicle budding induced by ESCRT complexes.
title_full_unstemmed	Membrane-elasticity model of Coatless vesicle budding induced by ESCRT complexes.
title_sort	membrane-elasticity model of coatless vesicle budding induced by escrt complexes.
publisher	Public Library of Science (PLoS)
publishDate	2012
url	https://doaj.org/article/05e2bb677dd04a869573878b655422b9
work_keys_str_mv	AT bartoszrozycki membraneelasticitymodelofcoatlessvesiclebuddinginducedbyescrtcomplexes AT evzenboura membraneelasticitymodelofcoatlessvesiclebuddinginducedbyescrtcomplexes AT jameshhurley membraneelasticitymodelofcoatlessvesiclebuddinginducedbyescrtcomplexes AT gerhardhummer membraneelasticitymodelofcoatlessvesiclebuddinginducedbyescrtcomplexes
_version_	1718424708752343040

Membrane-elasticity model of Coatless vesicle budding induced by ESCRT complexes.

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