Molecular characterization of Salmonella spp. and Listeria monocytogenes strains from biofilms in cattle and poultry slaughterhouses located in the federal District and State of Goiás, Brazil.

Listeria monocytogenes and Salmonella spp. are considered important foodborne pathogens that are commonly associated with foods of animal origin. The aim of this study was to perform molecular characterization of L. monocytogenes and Salmonella spp. isolated from biofilms of cattle and poultry slaug...

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Autores principales:	Emilia Fernanda Agostinho Davanzo, Rebecca Lavarini Dos Santos, Virgilio Hipólito de Lemos Castro, Joana Marchesini Palma, Bruno Rocha Pribul, Bruno Stéfano Lima Dallago, Bruna Fuga, Margareti Medeiros, Simoneide Souza Titze de Almeida, Hayanna Maria Boaventura da Costa, Dália Dos Prazeres Rodrigues, Nilton Lincopan, Simone Perecmanis, Angela Patrícia Santana
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/05e424281e684131b9ca319829143eef
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spelling	oai:doaj.org-article:05e424281e684131b9ca319829143eef2021-12-02T20:13:13ZMolecular characterization of Salmonella spp. and Listeria monocytogenes strains from biofilms in cattle and poultry slaughterhouses located in the federal District and State of Goiás, Brazil.1932-620310.1371/journal.pone.0259687https://doaj.org/article/05e424281e684131b9ca319829143eef2021-01-01T00:00:00Zhttps://doi.org/10.1371/journal.pone.0259687https://doaj.org/toc/1932-6203Listeria monocytogenes and Salmonella spp. are considered important foodborne pathogens that are commonly associated with foods of animal origin. The aim of this study was to perform molecular characterization of L. monocytogenes and Salmonella spp. isolated from biofilms of cattle and poultry slaughterhouses located in the Federal District and State of Goiás, Brazil. Fourteen L. monocytogenes isolates and one Salmonella sp. were detected in poultry slaughterhouses. No isolates were detected in cattle slaughterhouses. All L. monocytogenes isolates belonged to lineage II, and 11 different pulsotypes were detected. Pulsed-field gel electrophoresis analysis revealed the dissemination of two strains within one plant, in addition to the regional dissemination of one of them. The Salmonella isolate was identified via whole genome sequencing as Salmonella enterica serovar Minnesota ST548. In the sequence analysis, no premature stop codons were detected in the inlA gene of Listeria. All isolates demonstrated the ability to adhere to Caco-2 cells, while 50% were capable of invading them. Antimicrobial resistance was detected in 57.1% of the L. monocytogenes isolates, and resistance to sulfonamide was the most common feature. The tetC, ermB, and tetM genes were detected, and four isolates were classified as multidrug-resistant. Salmonella sp. was resistant to nine antimicrobials and was classified as multidrug-resistant. Resistance genes qnrB19, blaCMY-2, aac(6')-Iaa, sul2, and tetA, and a mutation in the parC gene were detected. The majority (78.5%) of the L. monocytogenes isolates were capable of forming biofilms after incubation at 37°C for 24 h, and 64.3% were capable of forming biofilms after incubation at 12°C for 168 h. There was no statistical difference in the biofilm-forming capacity under the different evaluated conditions. Salmonella sp. was capable of forming biofilms at both tested temperatures. Biofilm characterization was confirmed by collecting the samples consistently, at the same sampling points, and by assessing biofilm formation in vitro. These results highlight the potential risk of cross-contamination in poultry slaughterhouses and the importance of surveillance and pathogen control maintenance programs within the meat production industry.Emilia Fernanda Agostinho DavanzoRebecca Lavarini Dos SantosVirgilio Hipólito de Lemos CastroJoana Marchesini PalmaBruno Rocha PribulBruno Stéfano Lima DallagoBruna FugaMargareti MedeirosSimoneide Souza Titze de AlmeidaHayanna Maria Boaventura da CostaDália Dos Prazeres RodriguesNilton LincopanSimone PerecmanisAngela Patrícia SantanaPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 16, Iss 11, p e0259687 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Emilia Fernanda Agostinho Davanzo Rebecca Lavarini Dos Santos Virgilio Hipólito de Lemos Castro Joana Marchesini Palma Bruno Rocha Pribul Bruno Stéfano Lima Dallago Bruna Fuga Margareti Medeiros Simoneide Souza Titze de Almeida Hayanna Maria Boaventura da Costa Dália Dos Prazeres Rodrigues Nilton Lincopan Simone Perecmanis Angela Patrícia Santana Molecular characterization of Salmonella spp. and Listeria monocytogenes strains from biofilms in cattle and poultry slaughterhouses located in the federal District and State of Goiás, Brazil.
description	Listeria monocytogenes and Salmonella spp. are considered important foodborne pathogens that are commonly associated with foods of animal origin. The aim of this study was to perform molecular characterization of L. monocytogenes and Salmonella spp. isolated from biofilms of cattle and poultry slaughterhouses located in the Federal District and State of Goiás, Brazil. Fourteen L. monocytogenes isolates and one Salmonella sp. were detected in poultry slaughterhouses. No isolates were detected in cattle slaughterhouses. All L. monocytogenes isolates belonged to lineage II, and 11 different pulsotypes were detected. Pulsed-field gel electrophoresis analysis revealed the dissemination of two strains within one plant, in addition to the regional dissemination of one of them. The Salmonella isolate was identified via whole genome sequencing as Salmonella enterica serovar Minnesota ST548. In the sequence analysis, no premature stop codons were detected in the inlA gene of Listeria. All isolates demonstrated the ability to adhere to Caco-2 cells, while 50% were capable of invading them. Antimicrobial resistance was detected in 57.1% of the L. monocytogenes isolates, and resistance to sulfonamide was the most common feature. The tetC, ermB, and tetM genes were detected, and four isolates were classified as multidrug-resistant. Salmonella sp. was resistant to nine antimicrobials and was classified as multidrug-resistant. Resistance genes qnrB19, blaCMY-2, aac(6')-Iaa, sul2, and tetA, and a mutation in the parC gene were detected. The majority (78.5%) of the L. monocytogenes isolates were capable of forming biofilms after incubation at 37°C for 24 h, and 64.3% were capable of forming biofilms after incubation at 12°C for 168 h. There was no statistical difference in the biofilm-forming capacity under the different evaluated conditions. Salmonella sp. was capable of forming biofilms at both tested temperatures. Biofilm characterization was confirmed by collecting the samples consistently, at the same sampling points, and by assessing biofilm formation in vitro. These results highlight the potential risk of cross-contamination in poultry slaughterhouses and the importance of surveillance and pathogen control maintenance programs within the meat production industry.
format	article
author	Emilia Fernanda Agostinho Davanzo Rebecca Lavarini Dos Santos Virgilio Hipólito de Lemos Castro Joana Marchesini Palma Bruno Rocha Pribul Bruno Stéfano Lima Dallago Bruna Fuga Margareti Medeiros Simoneide Souza Titze de Almeida Hayanna Maria Boaventura da Costa Dália Dos Prazeres Rodrigues Nilton Lincopan Simone Perecmanis Angela Patrícia Santana
author_facet	Emilia Fernanda Agostinho Davanzo Rebecca Lavarini Dos Santos Virgilio Hipólito de Lemos Castro Joana Marchesini Palma Bruno Rocha Pribul Bruno Stéfano Lima Dallago Bruna Fuga Margareti Medeiros Simoneide Souza Titze de Almeida Hayanna Maria Boaventura da Costa Dália Dos Prazeres Rodrigues Nilton Lincopan Simone Perecmanis Angela Patrícia Santana
author_sort	Emilia Fernanda Agostinho Davanzo
title	Molecular characterization of Salmonella spp. and Listeria monocytogenes strains from biofilms in cattle and poultry slaughterhouses located in the federal District and State of Goiás, Brazil.
title_short	Molecular characterization of Salmonella spp. and Listeria monocytogenes strains from biofilms in cattle and poultry slaughterhouses located in the federal District and State of Goiás, Brazil.
title_full	Molecular characterization of Salmonella spp. and Listeria monocytogenes strains from biofilms in cattle and poultry slaughterhouses located in the federal District and State of Goiás, Brazil.
title_fullStr	Molecular characterization of Salmonella spp. and Listeria monocytogenes strains from biofilms in cattle and poultry slaughterhouses located in the federal District and State of Goiás, Brazil.
title_full_unstemmed	Molecular characterization of Salmonella spp. and Listeria monocytogenes strains from biofilms in cattle and poultry slaughterhouses located in the federal District and State of Goiás, Brazil.
title_sort	molecular characterization of salmonella spp. and listeria monocytogenes strains from biofilms in cattle and poultry slaughterhouses located in the federal district and state of goiás, brazil.
publisher	Public Library of Science (PLoS)
publishDate	2021
url	https://doaj.org/article/05e424281e684131b9ca319829143eef
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Molecular characterization of Salmonella spp. and Listeria monocytogenes strains from biofilms in cattle and poultry slaughterhouses located in the federal District and State of Goiás, Brazil.

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