EGCG impedes human Tau aggregation and interacts with Tau

Abstract Tau aggregation and accumulation is a key event in the pathogenesis of Alzheimer’s disease. Inhibition of Tau aggregation is therefore a potential therapeutic strategy to ameliorate the disease. Phytochemicals are being highlighted as potential aggregation inhibitors. Epigallocatechin-3-gal...

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Autores principales:	Shweta Kishor Sonawane, Hariharakrishnan Chidambaram, Debjyoti Boral, Nalini Vijay Gorantla, Abhishek Ankur Balmik, Abha Dangi, Sureshkumar Ramasamy, Udaya Kiran Marelli, Subashchandrabose Chinnathambi
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2020
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/05e91abcad094074ac9bbd912436a094
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spelling	oai:doaj.org-article:05e91abcad094074ac9bbd912436a0942021-12-02T16:06:39ZEGCG impedes human Tau aggregation and interacts with Tau10.1038/s41598-020-69429-62045-2322https://doaj.org/article/05e91abcad094074ac9bbd912436a0942020-07-01T00:00:00Zhttps://doi.org/10.1038/s41598-020-69429-6https://doaj.org/toc/2045-2322Abstract Tau aggregation and accumulation is a key event in the pathogenesis of Alzheimer’s disease. Inhibition of Tau aggregation is therefore a potential therapeutic strategy to ameliorate the disease. Phytochemicals are being highlighted as potential aggregation inhibitors. Epigallocatechin-3-gallate (EGCG) is an active phytochemical of green tea that has shown its potency against various diseases including aggregation inhibition of repeat Tau. The potency of EGCG in altering the PHF assembly of full-length human Tau has not been fully explored. By various biophysical and biochemical analyses like ThS fluorescence assay, MALDI-TOF analysis and Isothermal Titration Calorimetry, we demonstrate dual effect of EGCG on aggregation inhibition and disassembly of full-length Tau and their binding affinity. The IC50 for Tau aggregation by EGCG was found to be 64.2 μM.Shweta Kishor SonawaneHariharakrishnan ChidambaramDebjyoti BoralNalini Vijay GorantlaAbhishek Ankur BalmikAbha DangiSureshkumar RamasamyUdaya Kiran MarelliSubashchandrabose ChinnathambiNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 10, Iss 1, Pp 1-17 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Shweta Kishor Sonawane Hariharakrishnan Chidambaram Debjyoti Boral Nalini Vijay Gorantla Abhishek Ankur Balmik Abha Dangi Sureshkumar Ramasamy Udaya Kiran Marelli Subashchandrabose Chinnathambi EGCG impedes human Tau aggregation and interacts with Tau
description	Abstract Tau aggregation and accumulation is a key event in the pathogenesis of Alzheimer’s disease. Inhibition of Tau aggregation is therefore a potential therapeutic strategy to ameliorate the disease. Phytochemicals are being highlighted as potential aggregation inhibitors. Epigallocatechin-3-gallate (EGCG) is an active phytochemical of green tea that has shown its potency against various diseases including aggregation inhibition of repeat Tau. The potency of EGCG in altering the PHF assembly of full-length human Tau has not been fully explored. By various biophysical and biochemical analyses like ThS fluorescence assay, MALDI-TOF analysis and Isothermal Titration Calorimetry, we demonstrate dual effect of EGCG on aggregation inhibition and disassembly of full-length Tau and their binding affinity. The IC50 for Tau aggregation by EGCG was found to be 64.2 μM.
format	article
author	Shweta Kishor Sonawane Hariharakrishnan Chidambaram Debjyoti Boral Nalini Vijay Gorantla Abhishek Ankur Balmik Abha Dangi Sureshkumar Ramasamy Udaya Kiran Marelli Subashchandrabose Chinnathambi
author_facet	Shweta Kishor Sonawane Hariharakrishnan Chidambaram Debjyoti Boral Nalini Vijay Gorantla Abhishek Ankur Balmik Abha Dangi Sureshkumar Ramasamy Udaya Kiran Marelli Subashchandrabose Chinnathambi
author_sort	Shweta Kishor Sonawane
title	EGCG impedes human Tau aggregation and interacts with Tau
title_short	EGCG impedes human Tau aggregation and interacts with Tau
title_full	EGCG impedes human Tau aggregation and interacts with Tau
title_fullStr	EGCG impedes human Tau aggregation and interacts with Tau
title_full_unstemmed	EGCG impedes human Tau aggregation and interacts with Tau
title_sort	egcg impedes human tau aggregation and interacts with tau
publisher	Nature Portfolio
publishDate	2020
url	https://doaj.org/article/05e91abcad094074ac9bbd912436a094
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EGCG impedes human Tau aggregation and interacts with Tau

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