Rapid detection of bacterial infection using a novel single-tube, four-colour flow cytometric method: Comparison with PCT and CRP

ABSTRACT: Background: A key factor behind the unnecessary use of antibiotics is the lack of rapid and accurate diagnostic tests. In this study, we developed a novel and fast flow cytometric single-tube method to detect bacterial infections within 30 minutes. Methods: Quantitative flow cytometric fo...

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Autores principales:	Jari Nuutila, Ulla Hohenthal, Jarmo Oksi, Päivi Jalava-Karvinen
Formato:	article
Lenguaje:	EN
Publicado:	Elsevier 2021
Materias:	Flow cytometry Host biomarker Differential diagnostic Bacterial infection Viral infection Medicine R Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/05ee94c8426e4df9a835bc69d2d4e8a9
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spelling	oai:doaj.org-article:05ee94c8426e4df9a835bc69d2d4e8a92021-11-28T04:33:31ZRapid detection of bacterial infection using a novel single-tube, four-colour flow cytometric method: Comparison with PCT and CRP2352-396410.1016/j.ebiom.2021.103724https://doaj.org/article/05ee94c8426e4df9a835bc69d2d4e8a92021-12-01T00:00:00Zhttp://www.sciencedirect.com/science/article/pii/S2352396421005181https://doaj.org/toc/2352-3964ABSTRACT: Background: A key factor behind the unnecessary use of antibiotics is the lack of rapid and accurate diagnostic tests. In this study, we developed a novel and fast flow cytometric single-tube method to detect bacterial infections within 30 minutes. Methods: Quantitative flow cytometric four-colour analysis of host biomarkers CD35, CD64, CD329, and MHC class I expression on neutrophils and lymphocytes was performed on samples taken from 841 febrile patients with suspected infection. Obtained data was incorporated into the four-colour bacterial infection (FCBI)-index, using the developed bacterial infection algorithm. Findings: In distinguishing between microbiologically confirmed bacterial (n = 193) and viral (n = 291) infections, the FCBI-index method was superior to serum C-reactive protein (CRP) and procalcitonin (PCT). In 269 confirmed viral respiratory tract infections, 43% (95% CI: 37–49%) of the patients had an increased FCBI-index, suggesting probable bacterial coinfection. Interpretation: The proposed FCBI-index test might be a potent additional tool when assessing appropriateness of empiric antibiotic treatment. Funding: This study has been financially supported by Turku University Hospital (Turku, Finland) and The Finnish Medical Foundation.Jari NuutilaUlla HohenthalJarmo OksiPäivi Jalava-KarvinenElsevierarticleFlow cytometryHost biomarkerDifferential diagnosticBacterial infectionViral infectionMedicineRMedicine (General)R5-920ENEBioMedicine, Vol 74, Iss , Pp 103724- (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Flow cytometry Host biomarker Differential diagnostic Bacterial infection Viral infection Medicine R Medicine (General) R5-920
spellingShingle	Flow cytometry Host biomarker Differential diagnostic Bacterial infection Viral infection Medicine R Medicine (General) R5-920 Jari Nuutila Ulla Hohenthal Jarmo Oksi Päivi Jalava-Karvinen Rapid detection of bacterial infection using a novel single-tube, four-colour flow cytometric method: Comparison with PCT and CRP
description	ABSTRACT: Background: A key factor behind the unnecessary use of antibiotics is the lack of rapid and accurate diagnostic tests. In this study, we developed a novel and fast flow cytometric single-tube method to detect bacterial infections within 30 minutes. Methods: Quantitative flow cytometric four-colour analysis of host biomarkers CD35, CD64, CD329, and MHC class I expression on neutrophils and lymphocytes was performed on samples taken from 841 febrile patients with suspected infection. Obtained data was incorporated into the four-colour bacterial infection (FCBI)-index, using the developed bacterial infection algorithm. Findings: In distinguishing between microbiologically confirmed bacterial (n = 193) and viral (n = 291) infections, the FCBI-index method was superior to serum C-reactive protein (CRP) and procalcitonin (PCT). In 269 confirmed viral respiratory tract infections, 43% (95% CI: 37–49%) of the patients had an increased FCBI-index, suggesting probable bacterial coinfection. Interpretation: The proposed FCBI-index test might be a potent additional tool when assessing appropriateness of empiric antibiotic treatment. Funding: This study has been financially supported by Turku University Hospital (Turku, Finland) and The Finnish Medical Foundation.
format	article
author	Jari Nuutila Ulla Hohenthal Jarmo Oksi Päivi Jalava-Karvinen
author_facet	Jari Nuutila Ulla Hohenthal Jarmo Oksi Päivi Jalava-Karvinen
author_sort	Jari Nuutila
title	Rapid detection of bacterial infection using a novel single-tube, four-colour flow cytometric method: Comparison with PCT and CRP
title_short	Rapid detection of bacterial infection using a novel single-tube, four-colour flow cytometric method: Comparison with PCT and CRP
title_full	Rapid detection of bacterial infection using a novel single-tube, four-colour flow cytometric method: Comparison with PCT and CRP
title_fullStr	Rapid detection of bacterial infection using a novel single-tube, four-colour flow cytometric method: Comparison with PCT and CRP
title_full_unstemmed	Rapid detection of bacterial infection using a novel single-tube, four-colour flow cytometric method: Comparison with PCT and CRP
title_sort	rapid detection of bacterial infection using a novel single-tube, four-colour flow cytometric method: comparison with pct and crp
publisher	Elsevier
publishDate	2021
url	https://doaj.org/article/05ee94c8426e4df9a835bc69d2d4e8a9
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Rapid detection of bacterial infection using a novel single-tube, four-colour flow cytometric method: Comparison with PCT and CRP

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