Screening for viral hemorrhagic septicemia virus in marine fish along the Norwegian coastal line.

Viral hemorrhagic septicemia virus (VHSV) infects a wide range of marine fish species. To study the occurrence of VHSV in wild marine fish populations in Norwegian coastal waters and fjord systems a total of 1927 fish from 39 different species were sampled through 5 research cruises conducted in 200...

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Autores principales:	Nina Sandlund, Britt Gjerset, Øivind Bergh, Ingebjørg Modahl, Niels Jørgen Olesen, Renate Johansen
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2014
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/05f5384a32bb4b04a6ac0dfdd16b867d
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spelling	oai:doaj.org-article:05f5384a32bb4b04a6ac0dfdd16b867d2021-11-25T05:59:40ZScreening for viral hemorrhagic septicemia virus in marine fish along the Norwegian coastal line.1932-620310.1371/journal.pone.0108529https://doaj.org/article/05f5384a32bb4b04a6ac0dfdd16b867d2014-01-01T00:00:00Zhttps://doi.org/10.1371/journal.pone.0108529https://doaj.org/toc/1932-6203Viral hemorrhagic septicemia virus (VHSV) infects a wide range of marine fish species. To study the occurrence of VHSV in wild marine fish populations in Norwegian coastal waters and fjord systems a total of 1927 fish from 39 different species were sampled through 5 research cruises conducted in 2009 to 2011. In total, VHSV was detected by rRT-PCR in twelve samples originating from Atlantic herring (Clupea harengus), haddock (Melanogrammus aeglefinus), whiting (Merlangius merlangus) and silvery pout (Gadiculus argenteus). All fish tested positive in gills while four herring and one silvery pout also tested positive in internal organs. Successful virus isolation in cell culture was only obtained from one pooled Atlantic herring sample which shows that today's PCR methodology have a much higher sensitivity than cell culture for detection of VHSV. Sequencing revealed that the positive samples belonged to VHSV genotype Ib and phylogenetic analysis shows that the isolate from Atlantic herring and silvery pout are closely related. All positive fish were sampled in the same area in the northern county of Finnmark. This is the first detection of VHSV in Atlantic herring this far north, and to our knowledge the first detection of VHSV in silvery pout. However, low prevalence of VHSV genotype Ib in Atlantic herring and other wild marine fish are well known in other parts of Europe. Earlier there have been a few reports of disease outbreaks in farmed rainbow trout with VHSV of genotype Ib, and our results show that there is a possibility of transfer of VHSV from wild to farmed fish along the Norwegian coast line. The impact of VHSV on wild fish is not well documented.Nina SandlundBritt GjersetØivind BerghIngebjørg ModahlNiels Jørgen OlesenRenate JohansenPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 9, Iss 9, p e108529 (2014)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Nina Sandlund Britt Gjerset Øivind Bergh Ingebjørg Modahl Niels Jørgen Olesen Renate Johansen Screening for viral hemorrhagic septicemia virus in marine fish along the Norwegian coastal line.
description	Viral hemorrhagic septicemia virus (VHSV) infects a wide range of marine fish species. To study the occurrence of VHSV in wild marine fish populations in Norwegian coastal waters and fjord systems a total of 1927 fish from 39 different species were sampled through 5 research cruises conducted in 2009 to 2011. In total, VHSV was detected by rRT-PCR in twelve samples originating from Atlantic herring (Clupea harengus), haddock (Melanogrammus aeglefinus), whiting (Merlangius merlangus) and silvery pout (Gadiculus argenteus). All fish tested positive in gills while four herring and one silvery pout also tested positive in internal organs. Successful virus isolation in cell culture was only obtained from one pooled Atlantic herring sample which shows that today's PCR methodology have a much higher sensitivity than cell culture for detection of VHSV. Sequencing revealed that the positive samples belonged to VHSV genotype Ib and phylogenetic analysis shows that the isolate from Atlantic herring and silvery pout are closely related. All positive fish were sampled in the same area in the northern county of Finnmark. This is the first detection of VHSV in Atlantic herring this far north, and to our knowledge the first detection of VHSV in silvery pout. However, low prevalence of VHSV genotype Ib in Atlantic herring and other wild marine fish are well known in other parts of Europe. Earlier there have been a few reports of disease outbreaks in farmed rainbow trout with VHSV of genotype Ib, and our results show that there is a possibility of transfer of VHSV from wild to farmed fish along the Norwegian coast line. The impact of VHSV on wild fish is not well documented.
format	article
author	Nina Sandlund Britt Gjerset Øivind Bergh Ingebjørg Modahl Niels Jørgen Olesen Renate Johansen
author_facet	Nina Sandlund Britt Gjerset Øivind Bergh Ingebjørg Modahl Niels Jørgen Olesen Renate Johansen
author_sort	Nina Sandlund
title	Screening for viral hemorrhagic septicemia virus in marine fish along the Norwegian coastal line.
title_short	Screening for viral hemorrhagic septicemia virus in marine fish along the Norwegian coastal line.
title_full	Screening for viral hemorrhagic septicemia virus in marine fish along the Norwegian coastal line.
title_fullStr	Screening for viral hemorrhagic septicemia virus in marine fish along the Norwegian coastal line.
title_full_unstemmed	Screening for viral hemorrhagic septicemia virus in marine fish along the Norwegian coastal line.
title_sort	screening for viral hemorrhagic septicemia virus in marine fish along the norwegian coastal line.
publisher	Public Library of Science (PLoS)
publishDate	2014
url	https://doaj.org/article/05f5384a32bb4b04a6ac0dfdd16b867d
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Screening for viral hemorrhagic septicemia virus in marine fish along the Norwegian coastal line.

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