C5a receptor deficiency alters energy utilization and fat storage.

<h4>Objective</h4>To investigate the impact of whole body C5a receptor (C5aR) deficiency on energy metabolism and fat storage.<h4>Design</h4>Male wildtype (WT) and C5aR knockout (C5aRKO) mice were fed a low fat (CHOW) or a high fat high sucrose diet-induced obesity (DIO) diet...

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Autores principales:	Christian Roy, Abhishek Gupta, Alexandre Fisette, Marc Lapointe, Pegah Poursharifi, Denis Richard, Huiling Lu, Bao Lu, Norma Gerard, Craig Gerard, Katherine Cianflone
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2013
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/05f72ef200c34b5798955bb30bbf2143
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spelling	oai:doaj.org-article:05f72ef200c34b5798955bb30bbf21432021-11-18T07:46:41ZC5a receptor deficiency alters energy utilization and fat storage.1932-620310.1371/journal.pone.0062531https://doaj.org/article/05f72ef200c34b5798955bb30bbf21432013-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/23667486/?tool=EBIhttps://doaj.org/toc/1932-6203<h4>Objective</h4>To investigate the impact of whole body C5a receptor (C5aR) deficiency on energy metabolism and fat storage.<h4>Design</h4>Male wildtype (WT) and C5aR knockout (C5aRKO) mice were fed a low fat (CHOW) or a high fat high sucrose diet-induced obesity (DIO) diet for 14 weeks. Body weight and food intake were measured weekly. Indirect calorimetry, dietary fatload clearance, insulin and glucose tolerance tests were also evaluated. Liver, muscle and adipose tissue mRNA gene expression were measured by RT-PCR.<h4>Results</h4>At week one and 12, C5aRKO mice on DIO had increased oxygen consumption. After 12 weeks, although food intake was comparable, C5aRKO mice had lower body weight (-7% CHOW, -12% DIO) as well as smaller gonadal (-38% CHOW, -36% DIO) and inguinal (-29% CHOW, -30% DIO) fat pads than their WT counterparts. Conversely, in WT mice, C5aR was upregulated in DIO vs CHOW diets in gonadal adipose tissue, muscle and liver, while C5L2 mRNA expression was lower in C5aRKO on both diet. Furthermore, blood analysis showed lower plasma triglyceride and non-esterified fatty acid levels in both C5aRKO groups, with faster postprandial triglyceride clearance after a fatload. Additionally, C5aRKO mice showed lower CD36 expression in gonadal and muscle on both diets, while DGAT1 expression was higher in gonadal (CHOW) and liver (CHOW and DIO) and PPARγ was increased in muscle and liver.<h4>Conclusion</h4>These observations point towards a role (either direct or indirect) for C5aR in energy expenditure and fat storage, suggesting a dual role for C5aR in metabolism as well as in immunity.Christian RoyAbhishek GuptaAlexandre FisetteMarc LapointePegah PoursharifiDenis RichardHuiling LuBao LuNorma GerardCraig GerardKatherine CianflonePublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 8, Iss 5, p e62531 (2013)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Christian Roy Abhishek Gupta Alexandre Fisette Marc Lapointe Pegah Poursharifi Denis Richard Huiling Lu Bao Lu Norma Gerard Craig Gerard Katherine Cianflone C5a receptor deficiency alters energy utilization and fat storage.
description	<h4>Objective</h4>To investigate the impact of whole body C5a receptor (C5aR) deficiency on energy metabolism and fat storage.<h4>Design</h4>Male wildtype (WT) and C5aR knockout (C5aRKO) mice were fed a low fat (CHOW) or a high fat high sucrose diet-induced obesity (DIO) diet for 14 weeks. Body weight and food intake were measured weekly. Indirect calorimetry, dietary fatload clearance, insulin and glucose tolerance tests were also evaluated. Liver, muscle and adipose tissue mRNA gene expression were measured by RT-PCR.<h4>Results</h4>At week one and 12, C5aRKO mice on DIO had increased oxygen consumption. After 12 weeks, although food intake was comparable, C5aRKO mice had lower body weight (-7% CHOW, -12% DIO) as well as smaller gonadal (-38% CHOW, -36% DIO) and inguinal (-29% CHOW, -30% DIO) fat pads than their WT counterparts. Conversely, in WT mice, C5aR was upregulated in DIO vs CHOW diets in gonadal adipose tissue, muscle and liver, while C5L2 mRNA expression was lower in C5aRKO on both diet. Furthermore, blood analysis showed lower plasma triglyceride and non-esterified fatty acid levels in both C5aRKO groups, with faster postprandial triglyceride clearance after a fatload. Additionally, C5aRKO mice showed lower CD36 expression in gonadal and muscle on both diets, while DGAT1 expression was higher in gonadal (CHOW) and liver (CHOW and DIO) and PPARγ was increased in muscle and liver.<h4>Conclusion</h4>These observations point towards a role (either direct or indirect) for C5aR in energy expenditure and fat storage, suggesting a dual role for C5aR in metabolism as well as in immunity.
format	article
author	Christian Roy Abhishek Gupta Alexandre Fisette Marc Lapointe Pegah Poursharifi Denis Richard Huiling Lu Bao Lu Norma Gerard Craig Gerard Katherine Cianflone
author_facet	Christian Roy Abhishek Gupta Alexandre Fisette Marc Lapointe Pegah Poursharifi Denis Richard Huiling Lu Bao Lu Norma Gerard Craig Gerard Katherine Cianflone
author_sort	Christian Roy
title	C5a receptor deficiency alters energy utilization and fat storage.
title_short	C5a receptor deficiency alters energy utilization and fat storage.
title_full	C5a receptor deficiency alters energy utilization and fat storage.
title_fullStr	C5a receptor deficiency alters energy utilization and fat storage.
title_full_unstemmed	C5a receptor deficiency alters energy utilization and fat storage.
title_sort	c5a receptor deficiency alters energy utilization and fat storage.
publisher	Public Library of Science (PLoS)
publishDate	2013
url	https://doaj.org/article/05f72ef200c34b5798955bb30bbf2143
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C5a receptor deficiency alters energy utilization and fat storage.

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