Characterization of Cryopreserved Canine Amniotic Membrane

Amniotic membrane is an effective corneal reconstruction material in veterinary surgery. Cryopreserved amniotic membrane is widely used in practice. Properties of cryopreserved canine amniotic membranes are currently not well studied. This study aimed to compare three properties between canine amnio...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Nathawan Withavatpongtorn, Nalinee Tuntivanich
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	dogs amnion cryopreservation ophthalmology biomechanical phenomena Chemical technology TP1-1185 Chemical engineering TP155-156
Acceso en línea:	https://doaj.org/article/05f7a978a2634ba7960a33e5185d4c4e
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:05f7a978a2634ba7960a33e5185d4c4e
record_format	dspace
spelling	oai:doaj.org-article:05f7a978a2634ba7960a33e5185d4c4e2021-11-25T18:19:36ZCharacterization of Cryopreserved Canine Amniotic Membrane10.3390/membranes111108242077-0375https://doaj.org/article/05f7a978a2634ba7960a33e5185d4c4e2021-10-01T00:00:00Zhttps://www.mdpi.com/2077-0375/11/11/824https://doaj.org/toc/2077-0375Amniotic membrane is an effective corneal reconstruction material in veterinary surgery. Cryopreserved amniotic membrane is widely used in practice. Properties of cryopreserved canine amniotic membranes are currently not well studied. This study aimed to compare three properties between canine amniotic membranes cryopreserved for 7 days and 30 days, including tensile strength, transparency, and cell viability. After their respective cryopreservation time, stress–strain curves of the cryopreserved membranes’ tensile strength were assessed using a universal testing machine. Both groups produced J-shaped stress–strain curves with statistically comparable parameters, including maximum stress, strain, and Young’s modulus. The percentage of cell viability was observed by trypan blue staining under a light microscope. Membrane transparency was tested with a spectrophotometer. Transparency tests showed high levels of light transmission and low haze, with no statistical difference between groups. Cell viability was statistically lower in the 30-day cryopreserved group. Tensile strength and transparency of cryopreserved CAM were not significantly impeded for up to 30 days. For CAM to be used as an alternative corneal transplant material in veterinary and regenerative medicine, further research on cell biology, biomechanical properties of the membrane, and cell viability should be conducted.Nathawan WithavatpongtornNalinee TuntivanichMDPI AGarticledogsamnioncryopreservationophthalmologybiomechanical phenomenaChemical technologyTP1-1185Chemical engineeringTP155-156ENMembranes, Vol 11, Iss 824, p 824 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	dogs amnion cryopreservation ophthalmology biomechanical phenomena Chemical technology TP1-1185 Chemical engineering TP155-156
spellingShingle	dogs amnion cryopreservation ophthalmology biomechanical phenomena Chemical technology TP1-1185 Chemical engineering TP155-156 Nathawan Withavatpongtorn Nalinee Tuntivanich Characterization of Cryopreserved Canine Amniotic Membrane
description	Amniotic membrane is an effective corneal reconstruction material in veterinary surgery. Cryopreserved amniotic membrane is widely used in practice. Properties of cryopreserved canine amniotic membranes are currently not well studied. This study aimed to compare three properties between canine amniotic membranes cryopreserved for 7 days and 30 days, including tensile strength, transparency, and cell viability. After their respective cryopreservation time, stress–strain curves of the cryopreserved membranes’ tensile strength were assessed using a universal testing machine. Both groups produced J-shaped stress–strain curves with statistically comparable parameters, including maximum stress, strain, and Young’s modulus. The percentage of cell viability was observed by trypan blue staining under a light microscope. Membrane transparency was tested with a spectrophotometer. Transparency tests showed high levels of light transmission and low haze, with no statistical difference between groups. Cell viability was statistically lower in the 30-day cryopreserved group. Tensile strength and transparency of cryopreserved CAM were not significantly impeded for up to 30 days. For CAM to be used as an alternative corneal transplant material in veterinary and regenerative medicine, further research on cell biology, biomechanical properties of the membrane, and cell viability should be conducted.
format	article
author	Nathawan Withavatpongtorn Nalinee Tuntivanich
author_facet	Nathawan Withavatpongtorn Nalinee Tuntivanich
author_sort	Nathawan Withavatpongtorn
title	Characterization of Cryopreserved Canine Amniotic Membrane
title_short	Characterization of Cryopreserved Canine Amniotic Membrane
title_full	Characterization of Cryopreserved Canine Amniotic Membrane
title_fullStr	Characterization of Cryopreserved Canine Amniotic Membrane
title_full_unstemmed	Characterization of Cryopreserved Canine Amniotic Membrane
title_sort	characterization of cryopreserved canine amniotic membrane
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/05f7a978a2634ba7960a33e5185d4c4e
work_keys_str_mv	AT nathawanwithavatpongtorn characterizationofcryopreservedcanineamnioticmembrane AT nalineetuntivanich characterizationofcryopreservedcanineamnioticmembrane
_version_	1718411300538679296

Characterization of Cryopreserved Canine Amniotic Membrane

Ejemplares similares