The silkrose of Bombyx mori effectively prevents vibriosis in penaeid prawns via the activation of innate immunity

Abstract We previously identified novel bioactive polysaccharides from Bactrocera cucurbitae and Antheraea yamamai that activate innate immunity in RAW264 murine macrophages. However, in terms of potential applications in the cultivation of prawns, there were problems with the availability of these...

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Autores principales:	Muhammad Fariz Zahir Ali, Indri Afriani Yasin, Takashi Ohta, Atsushi Hashizume, Atsushi Ido, Takayuki Takahashi, Chiemi Miura, Takeshi Miura
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2018
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/05f9d1b57dac40689c3b2f3f1a545e49
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spelling	oai:doaj.org-article:05f9d1b57dac40689c3b2f3f1a545e492021-12-02T11:40:36ZThe silkrose of Bombyx mori effectively prevents vibriosis in penaeid prawns via the activation of innate immunity10.1038/s41598-018-27241-32045-2322https://doaj.org/article/05f9d1b57dac40689c3b2f3f1a545e492018-06-01T00:00:00Zhttps://doi.org/10.1038/s41598-018-27241-3https://doaj.org/toc/2045-2322Abstract We previously identified novel bioactive polysaccharides from Bactrocera cucurbitae and Antheraea yamamai that activate innate immunity in RAW264 murine macrophages. However, in terms of potential applications in the cultivation of prawns, there were problems with the availability of these insects. However, we have now identified a polysaccharide from Bombyx mori that activates innate immunity in RAW264 cells and penaeid prawns. This purified polysaccharide, termed silkrose of B. mori (silkrose-BM), has a molecular weight of 1,150,000 and produces a single symmetrical peak on HPLC. Eight of nine constitutive monosaccharides of silkrose-BM are concomitant with dipterose of B. cucurbitae (dipterose-BC) and silkrose of A. yamamai (silkrose-AY). The major differences are found in the molar ratios of the monosaccharides. Silkrose-BM is approximately 500-fold less potent than silkrose-AY (EC50: 2.5 and 0.0043 μg/mL, respectively) in a nitrite oxide (NO) production assay using RAW264 cells. However, the maximum NO production for silkrose-BM and AY were comparable and higher than that of the lipopolysaccharide of Escherichia coli. The survival of penaeid prawns (Litopenaeus vannamei and Marsupenaeus japonicus) after infection with Vibrio penaecida was significantly improved by both dietary silkrose-BM and B. mori pupae. This suggests that silkrose-BM effectively prevents vibriosis in penaeid prawns via the activation of innate immunity.Muhammad Fariz Zahir AliIndri Afriani YasinTakashi OhtaAtsushi HashizumeAtsushi IdoTakayuki TakahashiChiemi MiuraTakeshi MiuraNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 8, Iss 1, Pp 1-8 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Muhammad Fariz Zahir Ali Indri Afriani Yasin Takashi Ohta Atsushi Hashizume Atsushi Ido Takayuki Takahashi Chiemi Miura Takeshi Miura The silkrose of Bombyx mori effectively prevents vibriosis in penaeid prawns via the activation of innate immunity
description	Abstract We previously identified novel bioactive polysaccharides from Bactrocera cucurbitae and Antheraea yamamai that activate innate immunity in RAW264 murine macrophages. However, in terms of potential applications in the cultivation of prawns, there were problems with the availability of these insects. However, we have now identified a polysaccharide from Bombyx mori that activates innate immunity in RAW264 cells and penaeid prawns. This purified polysaccharide, termed silkrose of B. mori (silkrose-BM), has a molecular weight of 1,150,000 and produces a single symmetrical peak on HPLC. Eight of nine constitutive monosaccharides of silkrose-BM are concomitant with dipterose of B. cucurbitae (dipterose-BC) and silkrose of A. yamamai (silkrose-AY). The major differences are found in the molar ratios of the monosaccharides. Silkrose-BM is approximately 500-fold less potent than silkrose-AY (EC50: 2.5 and 0.0043 μg/mL, respectively) in a nitrite oxide (NO) production assay using RAW264 cells. However, the maximum NO production for silkrose-BM and AY were comparable and higher than that of the lipopolysaccharide of Escherichia coli. The survival of penaeid prawns (Litopenaeus vannamei and Marsupenaeus japonicus) after infection with Vibrio penaecida was significantly improved by both dietary silkrose-BM and B. mori pupae. This suggests that silkrose-BM effectively prevents vibriosis in penaeid prawns via the activation of innate immunity.
format	article
author	Muhammad Fariz Zahir Ali Indri Afriani Yasin Takashi Ohta Atsushi Hashizume Atsushi Ido Takayuki Takahashi Chiemi Miura Takeshi Miura
author_facet	Muhammad Fariz Zahir Ali Indri Afriani Yasin Takashi Ohta Atsushi Hashizume Atsushi Ido Takayuki Takahashi Chiemi Miura Takeshi Miura
author_sort	Muhammad Fariz Zahir Ali
title	The silkrose of Bombyx mori effectively prevents vibriosis in penaeid prawns via the activation of innate immunity
title_short	The silkrose of Bombyx mori effectively prevents vibriosis in penaeid prawns via the activation of innate immunity
title_full	The silkrose of Bombyx mori effectively prevents vibriosis in penaeid prawns via the activation of innate immunity
title_fullStr	The silkrose of Bombyx mori effectively prevents vibriosis in penaeid prawns via the activation of innate immunity
title_full_unstemmed	The silkrose of Bombyx mori effectively prevents vibriosis in penaeid prawns via the activation of innate immunity
title_sort	silkrose of bombyx mori effectively prevents vibriosis in penaeid prawns via the activation of innate immunity
publisher	Nature Portfolio
publishDate	2018
url	https://doaj.org/article/05f9d1b57dac40689c3b2f3f1a545e49
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The silkrose of Bombyx mori effectively prevents vibriosis in penaeid prawns via the activation of innate immunity

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