Linc00467 promotion of gastric cancer development by directly regulating miR-7-5p expression and downstream epidermal growth factor receptor

Linc00467 is a vital regulator in tumor progression. This study explores the molecular mechanisms of linc00467 in gastric cancer (GC). Linc00467 expression was obtained and analyzed in GC tissue through exploration in the cancer genome atlas database. Then, real-time quantitative polymerase chain re...

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Autores principales:	Li-Hao Deng, Hui Zhao, Li-Ping Bai, Jun Xie, Kai Liu, Feng Yan
Formato:	article
Lenguaje:	EN
Publicado:	Taylor & Francis Group 2021
Materias:	gastric cancer linc00467 mir-7-5p epidermal growth factor receptor proliferation migration invasion Biotechnology TP248.13-248.65
Acceso en línea:	https://doaj.org/article/0603bfa97c874438a6aea29cc24c006a
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spelling	oai:doaj.org-article:0603bfa97c874438a6aea29cc24c006a2021-11-04T15:51:54ZLinc00467 promotion of gastric cancer development by directly regulating miR-7-5p expression and downstream epidermal growth factor receptor2165-59792165-598710.1080/21655979.2021.1996014https://doaj.org/article/0603bfa97c874438a6aea29cc24c006a2021-10-01T00:00:00Zhttp://dx.doi.org/10.1080/21655979.2021.1996014https://doaj.org/toc/2165-5979https://doaj.org/toc/2165-5987Linc00467 is a vital regulator in tumor progression. This study explores the molecular mechanisms of linc00467 in gastric cancer (GC). Linc00467 expression was obtained and analyzed in GC tissue through exploration in the cancer genome atlas database. Then, real-time quantitative polymerase chain reaction was used to detect linc000467 expression in GC cells. Cell functions were observed using cell counting Kit-8, Transwell assay, Western blotting to testify the proliferation, migration, invasion, and the relative expression of epidermal growth factor receptor (EGFR) in GC cells. Moreover, a dual-luciferase reporter gene assay was used to verify the relationship between linc00467 and miR-7-5p. Results showed that the expression of linc00467 was overexpressed in GC. Linc00467 silencing decreased the GC cell proliferation, migration, and invasion. With mRNA verification and combined previous research, linc00467 directly regulated the miR-7-5p expression and downstream EGFR expression. Inhibited miR-7-5p could restore cell function, EGFR expression of GC cells when linc00467 knockdown occurs. Altogether, linc00467 directly regulates the miR-7-5p and EGFR signaling pathway to promote GC proliferation, migration, and invasion.Li-Hao DengHui ZhaoLi-Ping BaiJun XieKai LiuFeng YanTaylor & Francis Grouparticlegastric cancerlinc00467mir-7-5pepidermal growth factor receptorproliferationmigrationinvasionBiotechnologyTP248.13-248.65ENBioengineered, Vol 0, Iss 0 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	gastric cancer linc00467 mir-7-5p epidermal growth factor receptor proliferation migration invasion Biotechnology TP248.13-248.65
spellingShingle	gastric cancer linc00467 mir-7-5p epidermal growth factor receptor proliferation migration invasion Biotechnology TP248.13-248.65 Li-Hao Deng Hui Zhao Li-Ping Bai Jun Xie Kai Liu Feng Yan Linc00467 promotion of gastric cancer development by directly regulating miR-7-5p expression and downstream epidermal growth factor receptor
description	Linc00467 is a vital regulator in tumor progression. This study explores the molecular mechanisms of linc00467 in gastric cancer (GC). Linc00467 expression was obtained and analyzed in GC tissue through exploration in the cancer genome atlas database. Then, real-time quantitative polymerase chain reaction was used to detect linc000467 expression in GC cells. Cell functions were observed using cell counting Kit-8, Transwell assay, Western blotting to testify the proliferation, migration, invasion, and the relative expression of epidermal growth factor receptor (EGFR) in GC cells. Moreover, a dual-luciferase reporter gene assay was used to verify the relationship between linc00467 and miR-7-5p. Results showed that the expression of linc00467 was overexpressed in GC. Linc00467 silencing decreased the GC cell proliferation, migration, and invasion. With mRNA verification and combined previous research, linc00467 directly regulated the miR-7-5p expression and downstream EGFR expression. Inhibited miR-7-5p could restore cell function, EGFR expression of GC cells when linc00467 knockdown occurs. Altogether, linc00467 directly regulates the miR-7-5p and EGFR signaling pathway to promote GC proliferation, migration, and invasion.
format	article
author	Li-Hao Deng Hui Zhao Li-Ping Bai Jun Xie Kai Liu Feng Yan
author_facet	Li-Hao Deng Hui Zhao Li-Ping Bai Jun Xie Kai Liu Feng Yan
author_sort	Li-Hao Deng
title	Linc00467 promotion of gastric cancer development by directly regulating miR-7-5p expression and downstream epidermal growth factor receptor
title_short	Linc00467 promotion of gastric cancer development by directly regulating miR-7-5p expression and downstream epidermal growth factor receptor
title_full	Linc00467 promotion of gastric cancer development by directly regulating miR-7-5p expression and downstream epidermal growth factor receptor
title_fullStr	Linc00467 promotion of gastric cancer development by directly regulating miR-7-5p expression and downstream epidermal growth factor receptor
title_full_unstemmed	Linc00467 promotion of gastric cancer development by directly regulating miR-7-5p expression and downstream epidermal growth factor receptor
title_sort	linc00467 promotion of gastric cancer development by directly regulating mir-7-5p expression and downstream epidermal growth factor receptor
publisher	Taylor & Francis Group
publishDate	2021
url	https://doaj.org/article/0603bfa97c874438a6aea29cc24c006a
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Linc00467 promotion of gastric cancer development by directly regulating miR-7-5p expression and downstream epidermal growth factor receptor

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