Extracellular vesicle concentrations of glial fibrillary acidic protein and neurofilament light measured 1 year after traumatic brain injury

Abstract Traumatic brain injury (TBI) is linked to long-term symptoms in a sub-set of patients who sustain an injury, but this risk is not universal, leading us and others to question the nature of individual variability in recovery trajectories. Extracellular vesicles (EVs) are a promising, novel a...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Spencer Flynn, Jacqueline Leete, Pashtun Shahim, Cassandra Pattinson, Vivian A. Guedes, Chen Lai, Christina Devoto, Bao-Xi Qu, Kisha Greer, Brian Moore, Andre van der Merwe, Vindhya Ekanayake, Jessica Gill, Leighton Chan
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/060759812983446bb2a2dd295eeb8c01
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:060759812983446bb2a2dd295eeb8c01
record_format	dspace
spelling	oai:doaj.org-article:060759812983446bb2a2dd295eeb8c012021-12-02T10:54:14ZExtracellular vesicle concentrations of glial fibrillary acidic protein and neurofilament light measured 1 year after traumatic brain injury10.1038/s41598-021-82875-02045-2322https://doaj.org/article/060759812983446bb2a2dd295eeb8c012021-02-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-82875-0https://doaj.org/toc/2045-2322Abstract Traumatic brain injury (TBI) is linked to long-term symptoms in a sub-set of patients who sustain an injury, but this risk is not universal, leading us and others to question the nature of individual variability in recovery trajectories. Extracellular vesicles (EVs) are a promising, novel avenue to identify blood-based biomarkers for TBI. Here, our aim was to determine if glial fibrillary acidic protein (GFAP) and neurofilament light (NfL) measured 1-year postinjury in EVs could distinguish patients from controls, and whether these biomarkers relate to TBI severity or recovery outcomes. EV GFAP and EV NfL were measured using an ultrasensitive assay in 72 TBI patients and 20 controls. EV GFAP concentrations were elevated in moderate and severe TBI compared to controls (p’s < 0.001) and could distinguish controls from moderate (AUC = 0.86) or severe TBI (AUC = 0.88). Increased EV GFAP and EV NfL levels were associated with lower 1-year Glasgow Outcome Scale–Extended (GOS-E) score (p’s < 0.05). These findings suggest that blood-derived EV concentrations of GFAP and NfL drawn even 1 year after injury are higher in TBI patients compared to controls, and are related to injury severity and poor recovery outcomes, suggesting that TBIs alter the activity of these biomarkers, likely contributing to individual variability in recovery.Spencer FlynnJacqueline LeetePashtun ShahimCassandra PattinsonVivian A. GuedesChen LaiChristina DevotoBao-Xi QuKisha GreerBrian MooreAndre van der MerweVindhya EkanayakeJessica GillLeighton ChanNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-8 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Spencer Flynn Jacqueline Leete Pashtun Shahim Cassandra Pattinson Vivian A. Guedes Chen Lai Christina Devoto Bao-Xi Qu Kisha Greer Brian Moore Andre van der Merwe Vindhya Ekanayake Jessica Gill Leighton Chan Extracellular vesicle concentrations of glial fibrillary acidic protein and neurofilament light measured 1 year after traumatic brain injury
description	Abstract Traumatic brain injury (TBI) is linked to long-term symptoms in a sub-set of patients who sustain an injury, but this risk is not universal, leading us and others to question the nature of individual variability in recovery trajectories. Extracellular vesicles (EVs) are a promising, novel avenue to identify blood-based biomarkers for TBI. Here, our aim was to determine if glial fibrillary acidic protein (GFAP) and neurofilament light (NfL) measured 1-year postinjury in EVs could distinguish patients from controls, and whether these biomarkers relate to TBI severity or recovery outcomes. EV GFAP and EV NfL were measured using an ultrasensitive assay in 72 TBI patients and 20 controls. EV GFAP concentrations were elevated in moderate and severe TBI compared to controls (p’s < 0.001) and could distinguish controls from moderate (AUC = 0.86) or severe TBI (AUC = 0.88). Increased EV GFAP and EV NfL levels were associated with lower 1-year Glasgow Outcome Scale–Extended (GOS-E) score (p’s < 0.05). These findings suggest that blood-derived EV concentrations of GFAP and NfL drawn even 1 year after injury are higher in TBI patients compared to controls, and are related to injury severity and poor recovery outcomes, suggesting that TBIs alter the activity of these biomarkers, likely contributing to individual variability in recovery.
format	article
author	Spencer Flynn Jacqueline Leete Pashtun Shahim Cassandra Pattinson Vivian A. Guedes Chen Lai Christina Devoto Bao-Xi Qu Kisha Greer Brian Moore Andre van der Merwe Vindhya Ekanayake Jessica Gill Leighton Chan
author_facet	Spencer Flynn Jacqueline Leete Pashtun Shahim Cassandra Pattinson Vivian A. Guedes Chen Lai Christina Devoto Bao-Xi Qu Kisha Greer Brian Moore Andre van der Merwe Vindhya Ekanayake Jessica Gill Leighton Chan
author_sort	Spencer Flynn
title	Extracellular vesicle concentrations of glial fibrillary acidic protein and neurofilament light measured 1 year after traumatic brain injury
title_short	Extracellular vesicle concentrations of glial fibrillary acidic protein and neurofilament light measured 1 year after traumatic brain injury
title_full	Extracellular vesicle concentrations of glial fibrillary acidic protein and neurofilament light measured 1 year after traumatic brain injury
title_fullStr	Extracellular vesicle concentrations of glial fibrillary acidic protein and neurofilament light measured 1 year after traumatic brain injury
title_full_unstemmed	Extracellular vesicle concentrations of glial fibrillary acidic protein and neurofilament light measured 1 year after traumatic brain injury
title_sort	extracellular vesicle concentrations of glial fibrillary acidic protein and neurofilament light measured 1 year after traumatic brain injury
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/060759812983446bb2a2dd295eeb8c01
work_keys_str_mv	AT spencerflynn extracellularvesicleconcentrationsofglialfibrillaryacidicproteinandneurofilamentlightmeasured1yearaftertraumaticbraininjury AT jacquelineleete extracellularvesicleconcentrationsofglialfibrillaryacidicproteinandneurofilamentlightmeasured1yearaftertraumaticbraininjury AT pashtunshahim extracellularvesicleconcentrationsofglialfibrillaryacidicproteinandneurofilamentlightmeasured1yearaftertraumaticbraininjury AT cassandrapattinson extracellularvesicleconcentrationsofglialfibrillaryacidicproteinandneurofilamentlightmeasured1yearaftertraumaticbraininjury AT vivianaguedes extracellularvesicleconcentrationsofglialfibrillaryacidicproteinandneurofilamentlightmeasured1yearaftertraumaticbraininjury AT chenlai extracellularvesicleconcentrationsofglialfibrillaryacidicproteinandneurofilamentlightmeasured1yearaftertraumaticbraininjury AT christinadevoto extracellularvesicleconcentrationsofglialfibrillaryacidicproteinandneurofilamentlightmeasured1yearaftertraumaticbraininjury AT baoxiqu extracellularvesicleconcentrationsofglialfibrillaryacidicproteinandneurofilamentlightmeasured1yearaftertraumaticbraininjury AT kishagreer extracellularvesicleconcentrationsofglialfibrillaryacidicproteinandneurofilamentlightmeasured1yearaftertraumaticbraininjury AT brianmoore extracellularvesicleconcentrationsofglialfibrillaryacidicproteinandneurofilamentlightmeasured1yearaftertraumaticbraininjury AT andrevandermerwe extracellularvesicleconcentrationsofglialfibrillaryacidicproteinandneurofilamentlightmeasured1yearaftertraumaticbraininjury AT vindhyaekanayake extracellularvesicleconcentrationsofglialfibrillaryacidicproteinandneurofilamentlightmeasured1yearaftertraumaticbraininjury AT jessicagill extracellularvesicleconcentrationsofglialfibrillaryacidicproteinandneurofilamentlightmeasured1yearaftertraumaticbraininjury AT leightonchan extracellularvesicleconcentrationsofglialfibrillaryacidicproteinandneurofilamentlightmeasured1yearaftertraumaticbraininjury
_version_	1718396450399846400

Extracellular vesicle concentrations of glial fibrillary acidic protein and neurofilament light measured 1 year after traumatic brain injury

Ejemplares similares