Molecular analysis of Pseudomonas aeruginosa strains isolated from cystic fibrosis patients

Abstract Pseudomonas aeruginosa is a severe bacterial pathogen. Due to the genetic flexibility among strains, chronic airways infection can lead to mortality among cystic fibrosis (CF) patients. It is essential to develop patient-specific therapy which will rely on phenotypic and genomic diversity....

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Autores principales:	Dariusz Jarych, Ewa Augustynowicz-Kopec, Agnieszka Iwanska, Pawel Parniewski, Marta Majchrzak
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/0608a96347a342b6b9538913b0ad7568
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spelling	oai:doaj.org-article:0608a96347a342b6b9538913b0ad75682021-12-02T16:06:44ZMolecular analysis of Pseudomonas aeruginosa strains isolated from cystic fibrosis patients10.1038/s41598-021-95034-22045-2322https://doaj.org/article/0608a96347a342b6b9538913b0ad75682021-07-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-95034-2https://doaj.org/toc/2045-2322Abstract Pseudomonas aeruginosa is a severe bacterial pathogen. Due to the genetic flexibility among strains, chronic airways infection can lead to mortality among cystic fibrosis (CF) patients. It is essential to develop patient-specific therapy which will rely on phenotypic and genomic diversity. The primary objective of this study was to assess the genomic variability of P. aeruginosa strains, using two different molecular techniques for tracking the epidemiological transmissions. This study applied a multiple-locus variable-number tandem-repeat (VNTR) analysis (MLVA) for an efficient genotyping of clinical P. aeruginosa strains isolated from CF patients and compared results with a TRS-PCR typing. The percentage similarity analysis was performed using the categorical multi-state coefficient and UPGMA method. Based on the MLVA and TRS-PCR group assessment, 43 P. aeruginosa strains/variants were detected among the 63 clinical isolates from eight CF patients. The study of P. aeruginosa isolates has revealed that during chronic bacterial infections, CF patients harbor different P. aeruginosa strains or variants within the same host over the years. P. aeruginosa genotypes diversity may result from infection with several strains and result from a microevolution process of an initially acquired strain. The TRS-PCR method proposed in this work can complement the MLVA scheme. It can also be used as a preliminary method for genetic typing of P. aeruginosa isolates in CF patients.Dariusz JarychEwa Augustynowicz-KopecAgnieszka IwanskaPawel ParniewskiMarta MajchrzakNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-9 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Dariusz Jarych Ewa Augustynowicz-Kopec Agnieszka Iwanska Pawel Parniewski Marta Majchrzak Molecular analysis of Pseudomonas aeruginosa strains isolated from cystic fibrosis patients
description	Abstract Pseudomonas aeruginosa is a severe bacterial pathogen. Due to the genetic flexibility among strains, chronic airways infection can lead to mortality among cystic fibrosis (CF) patients. It is essential to develop patient-specific therapy which will rely on phenotypic and genomic diversity. The primary objective of this study was to assess the genomic variability of P. aeruginosa strains, using two different molecular techniques for tracking the epidemiological transmissions. This study applied a multiple-locus variable-number tandem-repeat (VNTR) analysis (MLVA) for an efficient genotyping of clinical P. aeruginosa strains isolated from CF patients and compared results with a TRS-PCR typing. The percentage similarity analysis was performed using the categorical multi-state coefficient and UPGMA method. Based on the MLVA and TRS-PCR group assessment, 43 P. aeruginosa strains/variants were detected among the 63 clinical isolates from eight CF patients. The study of P. aeruginosa isolates has revealed that during chronic bacterial infections, CF patients harbor different P. aeruginosa strains or variants within the same host over the years. P. aeruginosa genotypes diversity may result from infection with several strains and result from a microevolution process of an initially acquired strain. The TRS-PCR method proposed in this work can complement the MLVA scheme. It can also be used as a preliminary method for genetic typing of P. aeruginosa isolates in CF patients.
format	article
author	Dariusz Jarych Ewa Augustynowicz-Kopec Agnieszka Iwanska Pawel Parniewski Marta Majchrzak
author_facet	Dariusz Jarych Ewa Augustynowicz-Kopec Agnieszka Iwanska Pawel Parniewski Marta Majchrzak
author_sort	Dariusz Jarych
title	Molecular analysis of Pseudomonas aeruginosa strains isolated from cystic fibrosis patients
title_short	Molecular analysis of Pseudomonas aeruginosa strains isolated from cystic fibrosis patients
title_full	Molecular analysis of Pseudomonas aeruginosa strains isolated from cystic fibrosis patients
title_fullStr	Molecular analysis of Pseudomonas aeruginosa strains isolated from cystic fibrosis patients
title_full_unstemmed	Molecular analysis of Pseudomonas aeruginosa strains isolated from cystic fibrosis patients
title_sort	molecular analysis of pseudomonas aeruginosa strains isolated from cystic fibrosis patients
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/0608a96347a342b6b9538913b0ad7568
work_keys_str_mv	AT dariuszjarych molecularanalysisofpseudomonasaeruginosastrainsisolatedfromcysticfibrosispatients AT ewaaugustynowiczkopec molecularanalysisofpseudomonasaeruginosastrainsisolatedfromcysticfibrosispatients AT agnieszkaiwanska molecularanalysisofpseudomonasaeruginosastrainsisolatedfromcysticfibrosispatients AT pawelparniewski molecularanalysisofpseudomonasaeruginosastrainsisolatedfromcysticfibrosispatients AT martamajchrzak molecularanalysisofpseudomonasaeruginosastrainsisolatedfromcysticfibrosispatients
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Molecular analysis of Pseudomonas aeruginosa strains isolated from cystic fibrosis patients

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