Self-assembled albumin nanoparticles for combination therapy in prostate cancer

Huibo Lian,1 Jinhui Wu,2 Yiqiao Hu,2 Hongqian Guo1 1Department of Urology, Drum Tower Hospital, Medical School of Nanjing University, 2State Key Laboratory of Pharmaceutical Biotechnology, Medical School of Nanjing University, Nanjing, Jiangsu, People’s Republic of China Abstract: Resist...

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Autores principales:	Lian H, Wu J, Hu Y, Guo H
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2017
Materias:	Prostate cancer Combination therapy Albumin nanoparticles. Photothermal and photodynamic therapy Chemotherapy Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/060bbc56ec7a4bc890e255a4043929a2
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spelling	oai:doaj.org-article:060bbc56ec7a4bc890e255a4043929a22021-12-02T04:33:05ZSelf-assembled albumin nanoparticles for combination therapy in prostate cancer1178-2013https://doaj.org/article/060bbc56ec7a4bc890e255a4043929a22017-10-01T00:00:00Zhttps://www.dovepress.com/self-assembled-albumin-nanoparticles-for-combination-therapy-in-prosta-peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013Huibo Lian,1 Jinhui Wu,2 Yiqiao Hu,2 Hongqian Guo1 1Department of Urology, Drum Tower Hospital, Medical School of Nanjing University, 2State Key Laboratory of Pharmaceutical Biotechnology, Medical School of Nanjing University, Nanjing, Jiangsu, People’s Republic of China Abstract: Resistance to regular treatment strategies is a big challenge in the treatment of castration-resistant prostate cancer. Combination of photothermal and photodynamic therapy (PTT/PDT) with chemotherapy offers unique advantages over monotherapy alone. However, free drugs, such as photosensitizers and chemotherapeutic agents, lack tumor-targeted accumulation and can be easily eliminated from the body. Moreover, most of the PTT drugs are hydrophobic and their organic solvents have in vivo toxicity, thereby limiting their potential in clinical translation. Herein, simple multifunctional nanoparticles (NPs) using IR780 (a near-infrared dye) and docetaxel (DTX)-loaded nanoplatform based on human serum albumin (HSA) (HSA@IR780@DTX) was developed for targeted imaging and for PTT/PDT with chemotherapy for the treatment of castration-resistant prostate cancer treatment. In this platform, HSA is a biocompatible nanocarrier that binds to both DTX and IR780. DTX and IR780, as hydrophobic drug, can induce the self-assembly of HSA proteins. Transmission electron microscopic imaging showed that NPs formed by self-assembly are spherical with a smooth surface with a hydrodynamic diameter of 146.5±10.8 nm. The cytotoxicity of HSA@IR780@DTX NPs with or without laser irradiation in prostate cancer cells (22RV1) was determined via CCK-8 assay. The antitumor effect of HSA@IR780@DTX plus laser irradiation was better than either HSA@IR780@DTX without laser exposure or single PTT heating induced by HSA@IR780 NPs under near-infrared laser, suggesting a significant combined effect in comparison to monotherapy. Near-infrared fluorescence imaging showed that HSA@IR780@DTX NPs could preferentially accumulate in tumors. In vivo therapeutic efficacy experiment showed that xenografted prostate tumors on mice treated with HSA@IR780@DTX plus near-infrared laser irradiation were completely inhibited, whereas tumors on mice treated with chemotherapy alone (HSA@DTX and HSA@IR780@DTX without laser) or PTT/PDT alone (HSA@IR780 with laser) showed moderate growth inhibition. Overall, HSA@IR780@DTX NPs showed notable targeting and theranostic potential for the treatment of castration-resistant prostate cancer. Keywords: prostate cancer, combination therapy, albumin nanoparticles, photothermal and photodynamic therapy, chemotherapyLian HWu JHu YGuo HDove Medical PressarticleProstate cancerCombination therapyAlbumin nanoparticles. Photothermal and photodynamic therapyChemotherapyMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol Volume 12, Pp 7777-7787 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Prostate cancer Combination therapy Albumin nanoparticles. Photothermal and photodynamic therapy Chemotherapy Medicine (General) R5-920
spellingShingle	Prostate cancer Combination therapy Albumin nanoparticles. Photothermal and photodynamic therapy Chemotherapy Medicine (General) R5-920 Lian H Wu J Hu Y Guo H Self-assembled albumin nanoparticles for combination therapy in prostate cancer
description	Huibo Lian,1 Jinhui Wu,2 Yiqiao Hu,2 Hongqian Guo1 1Department of Urology, Drum Tower Hospital, Medical School of Nanjing University, 2State Key Laboratory of Pharmaceutical Biotechnology, Medical School of Nanjing University, Nanjing, Jiangsu, People’s Republic of China Abstract: Resistance to regular treatment strategies is a big challenge in the treatment of castration-resistant prostate cancer. Combination of photothermal and photodynamic therapy (PTT/PDT) with chemotherapy offers unique advantages over monotherapy alone. However, free drugs, such as photosensitizers and chemotherapeutic agents, lack tumor-targeted accumulation and can be easily eliminated from the body. Moreover, most of the PTT drugs are hydrophobic and their organic solvents have in vivo toxicity, thereby limiting their potential in clinical translation. Herein, simple multifunctional nanoparticles (NPs) using IR780 (a near-infrared dye) and docetaxel (DTX)-loaded nanoplatform based on human serum albumin (HSA) (HSA@IR780@DTX) was developed for targeted imaging and for PTT/PDT with chemotherapy for the treatment of castration-resistant prostate cancer treatment. In this platform, HSA is a biocompatible nanocarrier that binds to both DTX and IR780. DTX and IR780, as hydrophobic drug, can induce the self-assembly of HSA proteins. Transmission electron microscopic imaging showed that NPs formed by self-assembly are spherical with a smooth surface with a hydrodynamic diameter of 146.5±10.8 nm. The cytotoxicity of HSA@IR780@DTX NPs with or without laser irradiation in prostate cancer cells (22RV1) was determined via CCK-8 assay. The antitumor effect of HSA@IR780@DTX plus laser irradiation was better than either HSA@IR780@DTX without laser exposure or single PTT heating induced by HSA@IR780 NPs under near-infrared laser, suggesting a significant combined effect in comparison to monotherapy. Near-infrared fluorescence imaging showed that HSA@IR780@DTX NPs could preferentially accumulate in tumors. In vivo therapeutic efficacy experiment showed that xenografted prostate tumors on mice treated with HSA@IR780@DTX plus near-infrared laser irradiation were completely inhibited, whereas tumors on mice treated with chemotherapy alone (HSA@DTX and HSA@IR780@DTX without laser) or PTT/PDT alone (HSA@IR780 with laser) showed moderate growth inhibition. Overall, HSA@IR780@DTX NPs showed notable targeting and theranostic potential for the treatment of castration-resistant prostate cancer. Keywords: prostate cancer, combination therapy, albumin nanoparticles, photothermal and photodynamic therapy, chemotherapy
format	article
author	Lian H Wu J Hu Y Guo H
author_facet	Lian H Wu J Hu Y Guo H
author_sort	Lian H
title	Self-assembled albumin nanoparticles for combination therapy in prostate cancer
title_short	Self-assembled albumin nanoparticles for combination therapy in prostate cancer
title_full	Self-assembled albumin nanoparticles for combination therapy in prostate cancer
title_fullStr	Self-assembled albumin nanoparticles for combination therapy in prostate cancer
title_full_unstemmed	Self-assembled albumin nanoparticles for combination therapy in prostate cancer
title_sort	self-assembled albumin nanoparticles for combination therapy in prostate cancer
publisher	Dove Medical Press
publishDate	2017
url	https://doaj.org/article/060bbc56ec7a4bc890e255a4043929a2
work_keys_str_mv	AT lianh selfassembledalbuminnanoparticlesforcombinationtherapyinprostatecancer AT wuj selfassembledalbuminnanoparticlesforcombinationtherapyinprostatecancer AT huy selfassembledalbuminnanoparticlesforcombinationtherapyinprostatecancer AT guoh selfassembledalbuminnanoparticlesforcombinationtherapyinprostatecancer
_version_	1718401168074342400

Self-assembled albumin nanoparticles for combination therapy in prostate cancer

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