A Simple Inner-Stopper Guarded Trephine for Creation of Uniform Keratectomy Wounds in Rodents

Abstract Purpose: Creating controllable, reproducible keratectomy wounds in rodent corneas can be a challenge due to their small size, thickness, and the lack of usual tools available for human eyes such as a vacuum trephine. The purpose of this work is to provide a consistent, reproducible corneal...

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Autores principales:	Peter B. Le, Fang Chen, David Myung
Formato:	article
Lenguaje:	EN
Publicado:	Knowledge E 2021
Materias:	anterior lamellar keratoplasty (alk) corneal defect model inner-stopper guarded trephine keratectomy rat corneal wound model trephine design Ophthalmology RE1-994
Acceso en línea:	https://doaj.org/article/060cc1b940fc47cf87e73d677e90a0da
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spelling	oai:doaj.org-article:060cc1b940fc47cf87e73d677e90a0da2021-11-09T08:05:35ZA Simple Inner-Stopper Guarded Trephine for Creation of Uniform Keratectomy Wounds in Rodents2008-20102008-322X10.18502/jovr.v16i4.9743https://doaj.org/article/060cc1b940fc47cf87e73d677e90a0da2021-10-01T00:00:00Zhttps://doi.org/10.18502/jovr.v16i4.9743https://doaj.org/toc/2008-2010https://doaj.org/toc/2008-322XAbstract Purpose: Creating controllable, reproducible keratectomy wounds in rodent corneas can be a challenge due to their small size, thickness, and the lack of usual tools available for human eyes such as a vacuum trephine. The purpose of this work is to provide a consistent, reproducible corneal stromal defect in rats using a simple, economical, and customized inner-stopper guarded trephine. Methods: The inner-stopper guarded trephine is used to induce a circular wound in rat corneas. After trephination, the corneal flap can be removed by manual dissection using a blunt spatula. We used optical coherence topography (OCT) to measure the defect wound depth induced in ex vivo rat eyes. Results: Despite a minor learning curve, this simple device enables depth control, reduces variability of manual keratectomy wound depth in rats, and decreases the risk for corneal perforation during keratectomy. Corneal defect creation was highly reproducible across different researchers and was independent of their surgical training. Conclusion: This inner-stopper guarded trephine can be utilized and applied to pre-clinical testing of a wide range of corneal wound healing therapies, including but not limited to biotherapeutics, corneal prosthetics, and regenerative technologies.Peter B. LeFang ChenDavid MyungKnowledge Earticleanterior lamellar keratoplasty (alk)corneal defect modelinner-stopper guarded trephinekeratectomyrat corneal wound modeltrephine designOphthalmologyRE1-994ENJournal of Ophthalmic & Vision Research, Vol 16, Iss 4, Pp 544-551 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	anterior lamellar keratoplasty (alk) corneal defect model inner-stopper guarded trephine keratectomy rat corneal wound model trephine design Ophthalmology RE1-994
spellingShingle	anterior lamellar keratoplasty (alk) corneal defect model inner-stopper guarded trephine keratectomy rat corneal wound model trephine design Ophthalmology RE1-994 Peter B. Le Fang Chen David Myung A Simple Inner-Stopper Guarded Trephine for Creation of Uniform Keratectomy Wounds in Rodents
description	Abstract Purpose: Creating controllable, reproducible keratectomy wounds in rodent corneas can be a challenge due to their small size, thickness, and the lack of usual tools available for human eyes such as a vacuum trephine. The purpose of this work is to provide a consistent, reproducible corneal stromal defect in rats using a simple, economical, and customized inner-stopper guarded trephine. Methods: The inner-stopper guarded trephine is used to induce a circular wound in rat corneas. After trephination, the corneal flap can be removed by manual dissection using a blunt spatula. We used optical coherence topography (OCT) to measure the defect wound depth induced in ex vivo rat eyes. Results: Despite a minor learning curve, this simple device enables depth control, reduces variability of manual keratectomy wound depth in rats, and decreases the risk for corneal perforation during keratectomy. Corneal defect creation was highly reproducible across different researchers and was independent of their surgical training. Conclusion: This inner-stopper guarded trephine can be utilized and applied to pre-clinical testing of a wide range of corneal wound healing therapies, including but not limited to biotherapeutics, corneal prosthetics, and regenerative technologies.
format	article
author	Peter B. Le Fang Chen David Myung
author_facet	Peter B. Le Fang Chen David Myung
author_sort	Peter B. Le
title	A Simple Inner-Stopper Guarded Trephine for Creation of Uniform Keratectomy Wounds in Rodents
title_short	A Simple Inner-Stopper Guarded Trephine for Creation of Uniform Keratectomy Wounds in Rodents
title_full	A Simple Inner-Stopper Guarded Trephine for Creation of Uniform Keratectomy Wounds in Rodents
title_fullStr	A Simple Inner-Stopper Guarded Trephine for Creation of Uniform Keratectomy Wounds in Rodents
title_full_unstemmed	A Simple Inner-Stopper Guarded Trephine for Creation of Uniform Keratectomy Wounds in Rodents
title_sort	simple inner-stopper guarded trephine for creation of uniform keratectomy wounds in rodents
publisher	Knowledge E
publishDate	2021
url	https://doaj.org/article/060cc1b940fc47cf87e73d677e90a0da
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A Simple Inner-Stopper Guarded Trephine for Creation of Uniform Keratectomy Wounds in Rodents

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