The Effects of Ibuprofen Cytoxic Dose on caspase-3, -8 and -9 Activity level in cervical cancer (Hela) cells

BACKGROUND AND OBJECTIVE: Studies have shown that ibuprofen can have an anti-cancer effect on cervical cells, although the mechanism of this effect is not well known in cellular and molecular terms. Accordingly, the aim of this study was to investigate the effect of cytotoxic concentration of ibupro...

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Autores principales:	E Ghadiri, R Ahmadi, E Rostami
Formato:	article
Lenguaje:	EN FA
Publicado:	Babol University of Medical Sciences 2019
Materias:	ibuprofen caspase-3 caspase-8 caspase-9 cervical cancer Medicine R Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/0619a749c3cb434991bb5112571bd6c0
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id	oai:doaj.org-article:0619a749c3cb434991bb5112571bd6c0
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spelling	oai:doaj.org-article:0619a749c3cb434991bb5112571bd6c02021-11-10T08:30:37ZThe Effects of Ibuprofen Cytoxic Dose on caspase-3, -8 and -9 Activity level in cervical cancer (Hela) cells1561-41072251-7170https://doaj.org/article/0619a749c3cb434991bb5112571bd6c02019-12-01T00:00:00Zhttp://jbums.org/article-1-8454-en.htmlhttps://doaj.org/toc/1561-4107https://doaj.org/toc/2251-7170BACKGROUND AND OBJECTIVE: Studies have shown that ibuprofen can have an anti-cancer effect on cervical cells, although the mechanism of this effect is not well known in cellular and molecular terms. Accordingly, the aim of this study was to investigate the effect of cytotoxic concentration of ibuprofen on the activity of caspases -3, -8 and -9 in cervical cancer (Hela) cells. METHODS: In this experimental-laboratory study, Hela cells were prepared from Pasteur Institute cell Bank and were divided into the control group and groups exposed to 0.01, 0.1, 1 and 10 mg/ml of Ibuprofen. Viability of cells was measured by MTT assay. The activity level of caspases-3, -8 and -9 was assessed by colorimetric method. The data were statistically analyzed using ANOVA. FINDINGS: The viability decreased significantly in cervical cancer cells exposed to 0.1 (76%), 1(64%) and 10(15%) mg/ml of ibuprofen compared to control group (100%)(p<0.05, p<0.001 and p<0.001 respectively). The caspases-3، -8 and -9 activity level increased significantly in cervical cancer cells exposed to IC50 dose of ibuprofen compared with control group (p<0.001, p<0.001 and p<0.01 respectively). CONCLUSION: The results of present study showed that ibuprofen is able to reduce the viability of cervical cancer cells in a dose-dependent pathway, and this pathway is induced by activating of caspases-3, -8 and -9.E GhadiriR AhmadiE RostamiBabol University of Medical Sciencesarticleibuprofen caspase-3caspase-8caspase-9cervical cancerMedicineRMedicine (General)R5-920ENFAMajallah-i Dānishgāh-i ̒Ulūm-i Pizishkī-i Bābul, Vol 21, Iss 1, Pp 372-377 (2019)
institution	DOAJ
collection	DOAJ
language	EN FA
topic	ibuprofen caspase-3 caspase-8 caspase-9 cervical cancer Medicine R Medicine (General) R5-920
spellingShingle	ibuprofen caspase-3 caspase-8 caspase-9 cervical cancer Medicine R Medicine (General) R5-920 E Ghadiri R Ahmadi E Rostami The Effects of Ibuprofen Cytoxic Dose on caspase-3, -8 and -9 Activity level in cervical cancer (Hela) cells
description	BACKGROUND AND OBJECTIVE: Studies have shown that ibuprofen can have an anti-cancer effect on cervical cells, although the mechanism of this effect is not well known in cellular and molecular terms. Accordingly, the aim of this study was to investigate the effect of cytotoxic concentration of ibuprofen on the activity of caspases -3, -8 and -9 in cervical cancer (Hela) cells. METHODS: In this experimental-laboratory study, Hela cells were prepared from Pasteur Institute cell Bank and were divided into the control group and groups exposed to 0.01, 0.1, 1 and 10 mg/ml of Ibuprofen. Viability of cells was measured by MTT assay. The activity level of caspases-3, -8 and -9 was assessed by colorimetric method. The data were statistically analyzed using ANOVA. FINDINGS: The viability decreased significantly in cervical cancer cells exposed to 0.1 (76%), 1(64%) and 10(15%) mg/ml of ibuprofen compared to control group (100%)(p<0.05, p<0.001 and p<0.001 respectively). The caspases-3، -8 and -9 activity level increased significantly in cervical cancer cells exposed to IC50 dose of ibuprofen compared with control group (p<0.001, p<0.001 and p<0.01 respectively). CONCLUSION: The results of present study showed that ibuprofen is able to reduce the viability of cervical cancer cells in a dose-dependent pathway, and this pathway is induced by activating of caspases-3, -8 and -9.
format	article
author	E Ghadiri R Ahmadi E Rostami
author_facet	E Ghadiri R Ahmadi E Rostami
author_sort	E Ghadiri
title	The Effects of Ibuprofen Cytoxic Dose on caspase-3, -8 and -9 Activity level in cervical cancer (Hela) cells
title_short	The Effects of Ibuprofen Cytoxic Dose on caspase-3, -8 and -9 Activity level in cervical cancer (Hela) cells
title_full	The Effects of Ibuprofen Cytoxic Dose on caspase-3, -8 and -9 Activity level in cervical cancer (Hela) cells
title_fullStr	The Effects of Ibuprofen Cytoxic Dose on caspase-3, -8 and -9 Activity level in cervical cancer (Hela) cells
title_full_unstemmed	The Effects of Ibuprofen Cytoxic Dose on caspase-3, -8 and -9 Activity level in cervical cancer (Hela) cells
title_sort	effects of ibuprofen cytoxic dose on caspase-3, -8 and -9 activity level in cervical cancer (hela) cells
publisher	Babol University of Medical Sciences
publishDate	2019
url	https://doaj.org/article/0619a749c3cb434991bb5112571bd6c0
work_keys_str_mv	AT eghadiri theeffectsofibuprofencytoxicdoseoncaspase38and9activitylevelincervicalcancerhelacells AT rahmadi theeffectsofibuprofencytoxicdoseoncaspase38and9activitylevelincervicalcancerhelacells AT erostami theeffectsofibuprofencytoxicdoseoncaspase38and9activitylevelincervicalcancerhelacells AT eghadiri effectsofibuprofencytoxicdoseoncaspase38and9activitylevelincervicalcancerhelacells AT rahmadi effectsofibuprofencytoxicdoseoncaspase38and9activitylevelincervicalcancerhelacells AT erostami effectsofibuprofencytoxicdoseoncaspase38and9activitylevelincervicalcancerhelacells
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The Effects of Ibuprofen Cytoxic Dose on caspase-3, -8 and -9 Activity level in cervical cancer (Hela) cells

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