Divergent Expression of SPARC, SPARC-L, and SCPP Genes During Jawed Vertebrate Cartilage Mineralization
While cartilage is an ancient tissue found both in protostomes and deuterostomes, its mineralization evolved more recently, within the vertebrate lineage. SPARC, SPARC-L, and the SCPP members (Secretory Calcium-binding PhosphoProtein genes which evolved from SPARC-L) are major players of dentine and...
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Frontiers Media S.A.
2021
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oai:doaj.org-article:061ef7a812644c0c86252d1b51a219aa2021-12-01T01:25:18ZDivergent Expression of SPARC, SPARC-L, and SCPP Genes During Jawed Vertebrate Cartilage Mineralization1664-802110.3389/fgene.2021.788346https://doaj.org/article/061ef7a812644c0c86252d1b51a219aa2021-11-01T00:00:00Zhttps://www.frontiersin.org/articles/10.3389/fgene.2021.788346/fullhttps://doaj.org/toc/1664-8021While cartilage is an ancient tissue found both in protostomes and deuterostomes, its mineralization evolved more recently, within the vertebrate lineage. SPARC, SPARC-L, and the SCPP members (Secretory Calcium-binding PhosphoProtein genes which evolved from SPARC-L) are major players of dentine and bone mineralization, but their involvement in the emergence of the vertebrate mineralized cartilage remains unclear. We performed in situ hybridization on mineralizing cartilaginous skeletal elements of the frog Xenopus tropicalis (Xt) and the shark Scyliorhinus canicula (Sc) to examine the expression of SPARC (present in both species), SPARC-L (present in Sc only) and the SCPP members (present in Xt only). We show that while mineralizing cartilage expresses SPARC (but not SPARC-L) in Sc, it expresses the SCPP genes (but not SPARC) in Xt, and propose two possible evolutionary scenarios to explain these opposite expression patterns. In spite of these genetic divergences, our data draw the attention on an overlooked and evolutionarily conserved peripheral cartilage subdomain expressing SPARC or the SCPP genes and exhibiting a high propensity to mineralize.Adrian RomeroNicolas LeursDavid MuñozMélanie Debiais-ThibaudSylvain MarcelliniFrontiers Media S.A.articleSPARCSPARC-LSCPPcartilage mineralizationXenopus tropicalisScyliorhinus caniculaGeneticsQH426-470ENFrontiers in Genetics, Vol 12 (2021) |
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SPARC SPARC-L SCPP cartilage mineralization Xenopus tropicalis Scyliorhinus canicula Genetics QH426-470 |
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SPARC SPARC-L SCPP cartilage mineralization Xenopus tropicalis Scyliorhinus canicula Genetics QH426-470 Adrian Romero Nicolas Leurs David Muñoz Mélanie Debiais-Thibaud Sylvain Marcellini Divergent Expression of SPARC, SPARC-L, and SCPP Genes During Jawed Vertebrate Cartilage Mineralization |
description |
While cartilage is an ancient tissue found both in protostomes and deuterostomes, its mineralization evolved more recently, within the vertebrate lineage. SPARC, SPARC-L, and the SCPP members (Secretory Calcium-binding PhosphoProtein genes which evolved from SPARC-L) are major players of dentine and bone mineralization, but their involvement in the emergence of the vertebrate mineralized cartilage remains unclear. We performed in situ hybridization on mineralizing cartilaginous skeletal elements of the frog Xenopus tropicalis (Xt) and the shark Scyliorhinus canicula (Sc) to examine the expression of SPARC (present in both species), SPARC-L (present in Sc only) and the SCPP members (present in Xt only). We show that while mineralizing cartilage expresses SPARC (but not SPARC-L) in Sc, it expresses the SCPP genes (but not SPARC) in Xt, and propose two possible evolutionary scenarios to explain these opposite expression patterns. In spite of these genetic divergences, our data draw the attention on an overlooked and evolutionarily conserved peripheral cartilage subdomain expressing SPARC or the SCPP genes and exhibiting a high propensity to mineralize. |
format |
article |
author |
Adrian Romero Nicolas Leurs David Muñoz Mélanie Debiais-Thibaud Sylvain Marcellini |
author_facet |
Adrian Romero Nicolas Leurs David Muñoz Mélanie Debiais-Thibaud Sylvain Marcellini |
author_sort |
Adrian Romero |
title |
Divergent Expression of SPARC, SPARC-L, and SCPP Genes During Jawed Vertebrate Cartilage Mineralization |
title_short |
Divergent Expression of SPARC, SPARC-L, and SCPP Genes During Jawed Vertebrate Cartilage Mineralization |
title_full |
Divergent Expression of SPARC, SPARC-L, and SCPP Genes During Jawed Vertebrate Cartilage Mineralization |
title_fullStr |
Divergent Expression of SPARC, SPARC-L, and SCPP Genes During Jawed Vertebrate Cartilage Mineralization |
title_full_unstemmed |
Divergent Expression of SPARC, SPARC-L, and SCPP Genes During Jawed Vertebrate Cartilage Mineralization |
title_sort |
divergent expression of sparc, sparc-l, and scpp genes during jawed vertebrate cartilage mineralization |
publisher |
Frontiers Media S.A. |
publishDate |
2021 |
url |
https://doaj.org/article/061ef7a812644c0c86252d1b51a219aa |
work_keys_str_mv |
AT adrianromero divergentexpressionofsparcsparclandscppgenesduringjawedvertebratecartilagemineralization AT nicolasleurs divergentexpressionofsparcsparclandscppgenesduringjawedvertebratecartilagemineralization AT davidmunoz divergentexpressionofsparcsparclandscppgenesduringjawedvertebratecartilagemineralization AT melaniedebiaisthibaud divergentexpressionofsparcsparclandscppgenesduringjawedvertebratecartilagemineralization AT sylvainmarcellini divergentexpressionofsparcsparclandscppgenesduringjawedvertebratecartilagemineralization |
_version_ |
1718405942017523712 |