Divergent Expression of SPARC, SPARC-L, and SCPP Genes During Jawed Vertebrate Cartilage Mineralization

While cartilage is an ancient tissue found both in protostomes and deuterostomes, its mineralization evolved more recently, within the vertebrate lineage. SPARC, SPARC-L, and the SCPP members (Secretory Calcium-binding PhosphoProtein genes which evolved from SPARC-L) are major players of dentine and...

Descripción completa

Guardado en:
Detalles Bibliográficos
Autores principales: Adrian Romero, Nicolas Leurs, David Muñoz, Mélanie Debiais-Thibaud, Sylvain Marcellini
Formato: article
Lenguaje:EN
Publicado: Frontiers Media S.A. 2021
Materias:
Acceso en línea:https://doaj.org/article/061ef7a812644c0c86252d1b51a219aa
Etiquetas: Agregar Etiqueta
Sin Etiquetas, Sea el primero en etiquetar este registro!
id oai:doaj.org-article:061ef7a812644c0c86252d1b51a219aa
record_format dspace
spelling oai:doaj.org-article:061ef7a812644c0c86252d1b51a219aa2021-12-01T01:25:18ZDivergent Expression of SPARC, SPARC-L, and SCPP Genes During Jawed Vertebrate Cartilage Mineralization1664-802110.3389/fgene.2021.788346https://doaj.org/article/061ef7a812644c0c86252d1b51a219aa2021-11-01T00:00:00Zhttps://www.frontiersin.org/articles/10.3389/fgene.2021.788346/fullhttps://doaj.org/toc/1664-8021While cartilage is an ancient tissue found both in protostomes and deuterostomes, its mineralization evolved more recently, within the vertebrate lineage. SPARC, SPARC-L, and the SCPP members (Secretory Calcium-binding PhosphoProtein genes which evolved from SPARC-L) are major players of dentine and bone mineralization, but their involvement in the emergence of the vertebrate mineralized cartilage remains unclear. We performed in situ hybridization on mineralizing cartilaginous skeletal elements of the frog Xenopus tropicalis (Xt) and the shark Scyliorhinus canicula (Sc) to examine the expression of SPARC (present in both species), SPARC-L (present in Sc only) and the SCPP members (present in Xt only). We show that while mineralizing cartilage expresses SPARC (but not SPARC-L) in Sc, it expresses the SCPP genes (but not SPARC) in Xt, and propose two possible evolutionary scenarios to explain these opposite expression patterns. In spite of these genetic divergences, our data draw the attention on an overlooked and evolutionarily conserved peripheral cartilage subdomain expressing SPARC or the SCPP genes and exhibiting a high propensity to mineralize.Adrian RomeroNicolas LeursDavid MuñozMélanie Debiais-ThibaudSylvain MarcelliniFrontiers Media S.A.articleSPARCSPARC-LSCPPcartilage mineralizationXenopus tropicalisScyliorhinus caniculaGeneticsQH426-470ENFrontiers in Genetics, Vol 12 (2021)
institution DOAJ
collection DOAJ
language EN
topic SPARC
SPARC-L
SCPP
cartilage mineralization
Xenopus tropicalis
Scyliorhinus canicula
Genetics
QH426-470
spellingShingle SPARC
SPARC-L
SCPP
cartilage mineralization
Xenopus tropicalis
Scyliorhinus canicula
Genetics
QH426-470
Adrian Romero
Nicolas Leurs
David Muñoz
Mélanie Debiais-Thibaud
Sylvain Marcellini
Divergent Expression of SPARC, SPARC-L, and SCPP Genes During Jawed Vertebrate Cartilage Mineralization
description While cartilage is an ancient tissue found both in protostomes and deuterostomes, its mineralization evolved more recently, within the vertebrate lineage. SPARC, SPARC-L, and the SCPP members (Secretory Calcium-binding PhosphoProtein genes which evolved from SPARC-L) are major players of dentine and bone mineralization, but their involvement in the emergence of the vertebrate mineralized cartilage remains unclear. We performed in situ hybridization on mineralizing cartilaginous skeletal elements of the frog Xenopus tropicalis (Xt) and the shark Scyliorhinus canicula (Sc) to examine the expression of SPARC (present in both species), SPARC-L (present in Sc only) and the SCPP members (present in Xt only). We show that while mineralizing cartilage expresses SPARC (but not SPARC-L) in Sc, it expresses the SCPP genes (but not SPARC) in Xt, and propose two possible evolutionary scenarios to explain these opposite expression patterns. In spite of these genetic divergences, our data draw the attention on an overlooked and evolutionarily conserved peripheral cartilage subdomain expressing SPARC or the SCPP genes and exhibiting a high propensity to mineralize.
format article
author Adrian Romero
Nicolas Leurs
David Muñoz
Mélanie Debiais-Thibaud
Sylvain Marcellini
author_facet Adrian Romero
Nicolas Leurs
David Muñoz
Mélanie Debiais-Thibaud
Sylvain Marcellini
author_sort Adrian Romero
title Divergent Expression of SPARC, SPARC-L, and SCPP Genes During Jawed Vertebrate Cartilage Mineralization
title_short Divergent Expression of SPARC, SPARC-L, and SCPP Genes During Jawed Vertebrate Cartilage Mineralization
title_full Divergent Expression of SPARC, SPARC-L, and SCPP Genes During Jawed Vertebrate Cartilage Mineralization
title_fullStr Divergent Expression of SPARC, SPARC-L, and SCPP Genes During Jawed Vertebrate Cartilage Mineralization
title_full_unstemmed Divergent Expression of SPARC, SPARC-L, and SCPP Genes During Jawed Vertebrate Cartilage Mineralization
title_sort divergent expression of sparc, sparc-l, and scpp genes during jawed vertebrate cartilage mineralization
publisher Frontiers Media S.A.
publishDate 2021
url https://doaj.org/article/061ef7a812644c0c86252d1b51a219aa
work_keys_str_mv AT adrianromero divergentexpressionofsparcsparclandscppgenesduringjawedvertebratecartilagemineralization
AT nicolasleurs divergentexpressionofsparcsparclandscppgenesduringjawedvertebratecartilagemineralization
AT davidmunoz divergentexpressionofsparcsparclandscppgenesduringjawedvertebratecartilagemineralization
AT melaniedebiaisthibaud divergentexpressionofsparcsparclandscppgenesduringjawedvertebratecartilagemineralization
AT sylvainmarcellini divergentexpressionofsparcsparclandscppgenesduringjawedvertebratecartilagemineralization
_version_ 1718405942017523712