Comparing microfluidics and ultrasonication as formulation methods for developing hempseed oil nanoemulsions for oral delivery applications

Abstract Emerging formulation technologies aimed to produce nanoemulsions with improved characteristics, such as stability are attractive endeavors; however, comparisons between competing technologies are lacking. In this study, two formulation techniques that employed ultrasound and microfluidic ap...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Farahnaz Fathordoobady, Natalia Sannikova, Yigong Guo, Anika Singh, David D. Kitts, Anubhav Pratap-Singh
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/0622d1c750b54facbc16418090400ddc
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:0622d1c750b54facbc16418090400ddc
record_format	dspace
spelling	oai:doaj.org-article:0622d1c750b54facbc16418090400ddc2021-12-02T11:46:06ZComparing microfluidics and ultrasonication as formulation methods for developing hempseed oil nanoemulsions for oral delivery applications10.1038/s41598-020-79161-w2045-2322https://doaj.org/article/0622d1c750b54facbc16418090400ddc2021-01-01T00:00:00Zhttps://doi.org/10.1038/s41598-020-79161-whttps://doaj.org/toc/2045-2322Abstract Emerging formulation technologies aimed to produce nanoemulsions with improved characteristics, such as stability are attractive endeavors; however, comparisons between competing technologies are lacking. In this study, two formulation techniques that employed ultrasound and microfluidic approaches, respectively, were examined for relative capacity to produce serviceable oil in water nanoemulsions, based on hempseed oil (HSO). The ultrasound method reached > 99.5% entrapment efficiency with nanoemulsions that had an average droplet size (Z-Ave) < 180 nm and polydispersity index (PDI) of 0.15 ± 0.04. Surfactant concentration (% w/v) was found to be a significant factor (p < 0.05) controlling the Z-Ave, PDI and zeta potential of these nanoparticles. On the other hand, the microfluidic approach produced smaller particles compared to ultrasonication, with good stability observed during storage at room temperature. The Z-Ave of < 62.0 nm was achieved for microfluidic nanoemulsions by adjusting the aqueous : organic flow rate ratio and total flow rate at 4:1 and 12 mL/min, respectively. Further analyses including a morphology examination, a simulated gastrointestinal release behavior study, transepithelial transport evaluations and a toxicity test, using a Caco2-cell model, were performed to assess the functionality of the prepared formulations. The results of this study conclude that both approaches of ultrasound and microfluidics have the capability to prepare an HSO-nanoemulsion formulation, with acceptable characteristics and stability for oral delivery applications.Farahnaz FathordoobadyNatalia SannikovaYigong GuoAnika SinghDavid D. KittsAnubhav Pratap-SinghNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-12 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Farahnaz Fathordoobady Natalia Sannikova Yigong Guo Anika Singh David D. Kitts Anubhav Pratap-Singh Comparing microfluidics and ultrasonication as formulation methods for developing hempseed oil nanoemulsions for oral delivery applications
description	Abstract Emerging formulation technologies aimed to produce nanoemulsions with improved characteristics, such as stability are attractive endeavors; however, comparisons between competing technologies are lacking. In this study, two formulation techniques that employed ultrasound and microfluidic approaches, respectively, were examined for relative capacity to produce serviceable oil in water nanoemulsions, based on hempseed oil (HSO). The ultrasound method reached > 99.5% entrapment efficiency with nanoemulsions that had an average droplet size (Z-Ave) < 180 nm and polydispersity index (PDI) of 0.15 ± 0.04. Surfactant concentration (% w/v) was found to be a significant factor (p < 0.05) controlling the Z-Ave, PDI and zeta potential of these nanoparticles. On the other hand, the microfluidic approach produced smaller particles compared to ultrasonication, with good stability observed during storage at room temperature. The Z-Ave of < 62.0 nm was achieved for microfluidic nanoemulsions by adjusting the aqueous : organic flow rate ratio and total flow rate at 4:1 and 12 mL/min, respectively. Further analyses including a morphology examination, a simulated gastrointestinal release behavior study, transepithelial transport evaluations and a toxicity test, using a Caco2-cell model, were performed to assess the functionality of the prepared formulations. The results of this study conclude that both approaches of ultrasound and microfluidics have the capability to prepare an HSO-nanoemulsion formulation, with acceptable characteristics and stability for oral delivery applications.
format	article
author	Farahnaz Fathordoobady Natalia Sannikova Yigong Guo Anika Singh David D. Kitts Anubhav Pratap-Singh
author_facet	Farahnaz Fathordoobady Natalia Sannikova Yigong Guo Anika Singh David D. Kitts Anubhav Pratap-Singh
author_sort	Farahnaz Fathordoobady
title	Comparing microfluidics and ultrasonication as formulation methods for developing hempseed oil nanoemulsions for oral delivery applications
title_short	Comparing microfluidics and ultrasonication as formulation methods for developing hempseed oil nanoemulsions for oral delivery applications
title_full	Comparing microfluidics and ultrasonication as formulation methods for developing hempseed oil nanoemulsions for oral delivery applications
title_fullStr	Comparing microfluidics and ultrasonication as formulation methods for developing hempseed oil nanoemulsions for oral delivery applications
title_full_unstemmed	Comparing microfluidics and ultrasonication as formulation methods for developing hempseed oil nanoemulsions for oral delivery applications
title_sort	comparing microfluidics and ultrasonication as formulation methods for developing hempseed oil nanoemulsions for oral delivery applications
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/0622d1c750b54facbc16418090400ddc
work_keys_str_mv	AT farahnazfathordoobady comparingmicrofluidicsandultrasonicationasformulationmethodsfordevelopinghempseedoilnanoemulsionsfororaldeliveryapplications AT nataliasannikova comparingmicrofluidicsandultrasonicationasformulationmethodsfordevelopinghempseedoilnanoemulsionsfororaldeliveryapplications AT yigongguo comparingmicrofluidicsandultrasonicationasformulationmethodsfordevelopinghempseedoilnanoemulsionsfororaldeliveryapplications AT anikasingh comparingmicrofluidicsandultrasonicationasformulationmethodsfordevelopinghempseedoilnanoemulsionsfororaldeliveryapplications AT daviddkitts comparingmicrofluidicsandultrasonicationasformulationmethodsfordevelopinghempseedoilnanoemulsionsfororaldeliveryapplications AT anubhavpratapsingh comparingmicrofluidicsandultrasonicationasformulationmethodsfordevelopinghempseedoilnanoemulsionsfororaldeliveryapplications
_version_	1718395205950898176

Comparing microfluidics and ultrasonication as formulation methods for developing hempseed oil nanoemulsions for oral delivery applications

Ejemplares similares