FERONIA and microtubules independently contribute to mechanical integrity in the Arabidopsis shoot.

To survive, cells must constantly resist mechanical stress. In plants, this involves the reinforcement of cell walls, notably through microtubule-dependent cellulose deposition. How wall sensing might contribute to this response is unknown. Here, we tested whether the microtubule response to stress...

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Autores principales:	Alice Malivert, Özer Erguvan, Antoine Chevallier, Antoine Dehem, Rodrigue Friaud, Mengying Liu, Marjolaine Martin, Théophile Peyraud, Olivier Hamant, Stéphane Verger
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2021
Materias:	Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/0629fafd130d4d11afe58c1317a26140
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spelling	oai:doaj.org-article:0629fafd130d4d11afe58c1317a261402021-12-02T19:54:42ZFERONIA and microtubules independently contribute to mechanical integrity in the Arabidopsis shoot.1544-91731545-788510.1371/journal.pbio.3001454https://doaj.org/article/0629fafd130d4d11afe58c1317a261402021-11-01T00:00:00Zhttps://doi.org/10.1371/journal.pbio.3001454https://doaj.org/toc/1544-9173https://doaj.org/toc/1545-7885To survive, cells must constantly resist mechanical stress. In plants, this involves the reinforcement of cell walls, notably through microtubule-dependent cellulose deposition. How wall sensing might contribute to this response is unknown. Here, we tested whether the microtubule response to stress acts downstream of known wall sensors. Using a multistep screen with 11 mutant lines, we identify FERONIA (FER) as the primary candidate for the cell's response to stress in the shoot. However, this does not imply that FER acts upstream of the microtubule response to stress. In fact, when performing mechanical perturbations, we instead show that the expected microtubule response to stress does not require FER. We reveal that the feronia phenotype can be partially rescued by reducing tensile stress levels. Conversely, in the absence of both microtubules and FER, cells appear to swell and burst. Altogether, this shows that the microtubule response to stress acts as an independent pathway to resist stress, in parallel to FER. We propose that both pathways are required to maintain the mechanical integrity of plant cells.Alice MalivertÖzer ErguvanAntoine ChevallierAntoine DehemRodrigue FriaudMengying LiuMarjolaine MartinThéophile PeyraudOlivier HamantStéphane VergerPublic Library of Science (PLoS)articleBiology (General)QH301-705.5ENPLoS Biology, Vol 19, Iss 11, p e3001454 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Biology (General) QH301-705.5
spellingShingle	Biology (General) QH301-705.5 Alice Malivert Özer Erguvan Antoine Chevallier Antoine Dehem Rodrigue Friaud Mengying Liu Marjolaine Martin Théophile Peyraud Olivier Hamant Stéphane Verger FERONIA and microtubules independently contribute to mechanical integrity in the Arabidopsis shoot.
description	To survive, cells must constantly resist mechanical stress. In plants, this involves the reinforcement of cell walls, notably through microtubule-dependent cellulose deposition. How wall sensing might contribute to this response is unknown. Here, we tested whether the microtubule response to stress acts downstream of known wall sensors. Using a multistep screen with 11 mutant lines, we identify FERONIA (FER) as the primary candidate for the cell's response to stress in the shoot. However, this does not imply that FER acts upstream of the microtubule response to stress. In fact, when performing mechanical perturbations, we instead show that the expected microtubule response to stress does not require FER. We reveal that the feronia phenotype can be partially rescued by reducing tensile stress levels. Conversely, in the absence of both microtubules and FER, cells appear to swell and burst. Altogether, this shows that the microtubule response to stress acts as an independent pathway to resist stress, in parallel to FER. We propose that both pathways are required to maintain the mechanical integrity of plant cells.
format	article
author	Alice Malivert Özer Erguvan Antoine Chevallier Antoine Dehem Rodrigue Friaud Mengying Liu Marjolaine Martin Théophile Peyraud Olivier Hamant Stéphane Verger
author_facet	Alice Malivert Özer Erguvan Antoine Chevallier Antoine Dehem Rodrigue Friaud Mengying Liu Marjolaine Martin Théophile Peyraud Olivier Hamant Stéphane Verger
author_sort	Alice Malivert
title	FERONIA and microtubules independently contribute to mechanical integrity in the Arabidopsis shoot.
title_short	FERONIA and microtubules independently contribute to mechanical integrity in the Arabidopsis shoot.
title_full	FERONIA and microtubules independently contribute to mechanical integrity in the Arabidopsis shoot.
title_fullStr	FERONIA and microtubules independently contribute to mechanical integrity in the Arabidopsis shoot.
title_full_unstemmed	FERONIA and microtubules independently contribute to mechanical integrity in the Arabidopsis shoot.
title_sort	feronia and microtubules independently contribute to mechanical integrity in the arabidopsis shoot.
publisher	Public Library of Science (PLoS)
publishDate	2021
url	https://doaj.org/article/0629fafd130d4d11afe58c1317a26140
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FERONIA and microtubules independently contribute to mechanical integrity in the Arabidopsis shoot.

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