Endoplasmic reticulum remodeling tunes IP₃-dependent Ca²+ release sensitivity.

The activation of vertebrate development at fertilization relies on IP₃-dependent Ca²⁺ release, a pathway that is sensitized during oocyte maturation. This sensitization has been shown to correlate with the remodeling of the endoplasmic reticulum into large ER patches, however the mechanisms involve...

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Autores principales:	Lu Sun, Fang Yu, Aman Ullah, Satanay Hubrack, Arwa Daalis, Peter Jung, Khaled Machaca
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2011
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/06319689fc764d83b837d80f62e97ca0
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id	oai:doaj.org-article:06319689fc764d83b837d80f62e97ca0
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spelling	oai:doaj.org-article:06319689fc764d83b837d80f62e97ca02021-11-18T07:33:22ZEndoplasmic reticulum remodeling tunes IP₃-dependent Ca²+ release sensitivity.1932-620310.1371/journal.pone.0027928https://doaj.org/article/06319689fc764d83b837d80f62e97ca02011-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/22140486/?tool=EBIhttps://doaj.org/toc/1932-6203The activation of vertebrate development at fertilization relies on IP₃-dependent Ca²⁺ release, a pathway that is sensitized during oocyte maturation. This sensitization has been shown to correlate with the remodeling of the endoplasmic reticulum into large ER patches, however the mechanisms involved are not clear. Here we show that IP₃ receptors within ER patches have a higher sensitivity to IP₃ than those in the neighboring reticular ER. The lateral diffusion rate of IP₃ receptors in both ER domains is similar, and ER patches dynamically fuse with reticular ER, arguing that IP₃ receptors exchange freely between the two ER compartments. These results suggest that increasing the density of IP₃ receptors through ER remodeling is sufficient to sensitize IP₃-dependent Ca²⁺ release. Mathematical modeling supports this concept of 'geometric sensitization' of IP₃ receptors as a population, and argues that it depends on enhanced Ca²⁺-dependent cooperativity at sub-threshold IP₃ concentrations. This represents a novel mechanism of tuning the sensitivity of IP₃ receptors through ER remodeling during meiosis.Lu SunFang YuAman UllahSatanay HubrackArwa DaalisPeter JungKhaled MachacaPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 6, Iss 11, p e27928 (2011)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Lu Sun Fang Yu Aman Ullah Satanay Hubrack Arwa Daalis Peter Jung Khaled Machaca Endoplasmic reticulum remodeling tunes IP₃-dependent Ca²+ release sensitivity.
description	The activation of vertebrate development at fertilization relies on IP₃-dependent Ca²⁺ release, a pathway that is sensitized during oocyte maturation. This sensitization has been shown to correlate with the remodeling of the endoplasmic reticulum into large ER patches, however the mechanisms involved are not clear. Here we show that IP₃ receptors within ER patches have a higher sensitivity to IP₃ than those in the neighboring reticular ER. The lateral diffusion rate of IP₃ receptors in both ER domains is similar, and ER patches dynamically fuse with reticular ER, arguing that IP₃ receptors exchange freely between the two ER compartments. These results suggest that increasing the density of IP₃ receptors through ER remodeling is sufficient to sensitize IP₃-dependent Ca²⁺ release. Mathematical modeling supports this concept of 'geometric sensitization' of IP₃ receptors as a population, and argues that it depends on enhanced Ca²⁺-dependent cooperativity at sub-threshold IP₃ concentrations. This represents a novel mechanism of tuning the sensitivity of IP₃ receptors through ER remodeling during meiosis.
format	article
author	Lu Sun Fang Yu Aman Ullah Satanay Hubrack Arwa Daalis Peter Jung Khaled Machaca
author_facet	Lu Sun Fang Yu Aman Ullah Satanay Hubrack Arwa Daalis Peter Jung Khaled Machaca
author_sort	Lu Sun
title	Endoplasmic reticulum remodeling tunes IP₃-dependent Ca²+ release sensitivity.
title_short	Endoplasmic reticulum remodeling tunes IP₃-dependent Ca²+ release sensitivity.
title_full	Endoplasmic reticulum remodeling tunes IP₃-dependent Ca²+ release sensitivity.
title_fullStr	Endoplasmic reticulum remodeling tunes IP₃-dependent Ca²+ release sensitivity.
title_full_unstemmed	Endoplasmic reticulum remodeling tunes IP₃-dependent Ca²+ release sensitivity.
title_sort	endoplasmic reticulum remodeling tunes ip₃-dependent ca²+ release sensitivity.
publisher	Public Library of Science (PLoS)
publishDate	2011
url	https://doaj.org/article/06319689fc764d83b837d80f62e97ca0
work_keys_str_mv	AT lusun endoplasmicreticulumremodelingtunesip3dependentca2releasesensitivity AT fangyu endoplasmicreticulumremodelingtunesip3dependentca2releasesensitivity AT amanullah endoplasmicreticulumremodelingtunesip3dependentca2releasesensitivity AT satanayhubrack endoplasmicreticulumremodelingtunesip3dependentca2releasesensitivity AT arwadaalis endoplasmicreticulumremodelingtunesip3dependentca2releasesensitivity AT peterjung endoplasmicreticulumremodelingtunesip3dependentca2releasesensitivity AT khaledmachaca endoplasmicreticulumremodelingtunesip3dependentca2releasesensitivity
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Endoplasmic reticulum remodeling tunes IP₃-dependent Ca²+ release sensitivity.

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