Medaka insulin-like growth factor-2 supports self-renewal of the embryonic stem cell line and blastomeres in vitro

Abstract Insulin-like growth factors (IGFs) regulate diverse processes including energy metabolism, cell proliferation and embryonic development. They activate the IGF signaling pathway via binding to cell surface receptors. Here we report an essential role of IGF2 in maintaining the pluripotency of...

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Autores principales:	Yongming Yuan, Yunhan Hong
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/06355cbd04db45fa8c2a902ea911a4b3
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spelling	oai:doaj.org-article:06355cbd04db45fa8c2a902ea911a4b32021-12-02T12:30:11ZMedaka insulin-like growth factor-2 supports self-renewal of the embryonic stem cell line and blastomeres in vitro10.1038/s41598-017-00094-y2045-2322https://doaj.org/article/06355cbd04db45fa8c2a902ea911a4b32017-03-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-00094-yhttps://doaj.org/toc/2045-2322Abstract Insulin-like growth factors (IGFs) regulate diverse processes including energy metabolism, cell proliferation and embryonic development. They activate the IGF signaling pathway via binding to cell surface receptors. Here we report an essential role of IGF2 in maintaining the pluripotency of embryonic stem (ES) cell from medaka (Oryzias latipes). The medaka igf2 gene was cloned for prokaryotically expression of IGF2 ligand and green fluorescent protein-tagged IGF2 namely IGF2:GFP. With flow cytometry analysis, we demonstrated that the IGF2:GFP can bind to the cultured ES cells from medaka and zebrafish respectively. We also verified that IGF2 is able to activate the phosphorylation of Erk1/2 and Akt, and sustain the viability and pluripotency of medaka ES cells in culture. Furthermore, we characterized the binding of IGF2:GFP to freshly isolated blastomeres by fluorescence microscopy and electron microscopy. Most importantly, we revealed the important role of IGF2 in supporting the derivation of blastomeres in short-term culture. Therefore, Medaka IGF2 is essential for the self-renewal of cultured ES cells and blastomeres from fish embryos. This finding underscores a conserved role of the IGF signaling pathway in stem cells from fish to mammals.Yongming YuanYunhan HongNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-11 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Yongming Yuan Yunhan Hong Medaka insulin-like growth factor-2 supports self-renewal of the embryonic stem cell line and blastomeres in vitro
description	Abstract Insulin-like growth factors (IGFs) regulate diverse processes including energy metabolism, cell proliferation and embryonic development. They activate the IGF signaling pathway via binding to cell surface receptors. Here we report an essential role of IGF2 in maintaining the pluripotency of embryonic stem (ES) cell from medaka (Oryzias latipes). The medaka igf2 gene was cloned for prokaryotically expression of IGF2 ligand and green fluorescent protein-tagged IGF2 namely IGF2:GFP. With flow cytometry analysis, we demonstrated that the IGF2:GFP can bind to the cultured ES cells from medaka and zebrafish respectively. We also verified that IGF2 is able to activate the phosphorylation of Erk1/2 and Akt, and sustain the viability and pluripotency of medaka ES cells in culture. Furthermore, we characterized the binding of IGF2:GFP to freshly isolated blastomeres by fluorescence microscopy and electron microscopy. Most importantly, we revealed the important role of IGF2 in supporting the derivation of blastomeres in short-term culture. Therefore, Medaka IGF2 is essential for the self-renewal of cultured ES cells and blastomeres from fish embryos. This finding underscores a conserved role of the IGF signaling pathway in stem cells from fish to mammals.
format	article
author	Yongming Yuan Yunhan Hong
author_facet	Yongming Yuan Yunhan Hong
author_sort	Yongming Yuan
title	Medaka insulin-like growth factor-2 supports self-renewal of the embryonic stem cell line and blastomeres in vitro
title_short	Medaka insulin-like growth factor-2 supports self-renewal of the embryonic stem cell line and blastomeres in vitro
title_full	Medaka insulin-like growth factor-2 supports self-renewal of the embryonic stem cell line and blastomeres in vitro
title_fullStr	Medaka insulin-like growth factor-2 supports self-renewal of the embryonic stem cell line and blastomeres in vitro
title_full_unstemmed	Medaka insulin-like growth factor-2 supports self-renewal of the embryonic stem cell line and blastomeres in vitro
title_sort	medaka insulin-like growth factor-2 supports self-renewal of the embryonic stem cell line and blastomeres in vitro
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/06355cbd04db45fa8c2a902ea911a4b3
work_keys_str_mv	AT yongmingyuan medakainsulinlikegrowthfactor2supportsselfrenewaloftheembryonicstemcelllineandblastomeresinvitro AT yunhanhong medakainsulinlikegrowthfactor2supportsselfrenewaloftheembryonicstemcelllineandblastomeresinvitro
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Medaka insulin-like growth factor-2 supports self-renewal of the embryonic stem cell line and blastomeres in vitro

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